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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pPEE36 Resource Report Resource Website |
RRID:Addgene_128376 | Neomycin (NEO) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 7 sequence (CNAG_05557 - CNAG_05558) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:46 | 0 | |
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pPEE35 Resource Report Resource Website |
RRID:Addgene_128375 | Neomycin (NEO) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 6 sequence (CNAG_04586 - CNAG_04585) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:46 | 0 | |
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pPEE34 Resource Report Resource Website |
RRID:Addgene_128374 | Neomycin (NEO) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 5 sequence (CNAG_05670 - CNAG_05671) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:46 | 0 | |
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pPEE39 Resource Report Resource Website |
RRID:Addgene_128379 | Hygromycin (HYG) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 5 sequence (CNAG_05670 - CNAG_05671) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:46 | 0 | |
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pPEE38 Resource Report Resource Website |
RRID:Addgene_128378 | Hygromycin (HYG) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 4 sequence (CNAG_06526 - CNAG_06527) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:46 | 0 | |
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pPEE37 Resource Report Resource Website |
RRID:Addgene_128377 | Hygromycin (HYG) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 3 sequence (CNAG_02589 - CNAG_02588) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:46 | 0 | |
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pLG371 Resource Report Resource Website |
RRID:Addgene_128410 | gfpmut2 | Synthetic | Ampicillin | PMID:31792444 | Vector Backbone:pLG166; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:47 | 0 | ||
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pPEE32 Resource Report Resource Website |
RRID:Addgene_128372 | Neomycin (NEO) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 3 sequence (CNAG_02589 - CNAG_02588) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:46 | 0 | |
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pPEE21 Resource Report Resource Website |
RRID:Addgene_128364 | Acetamidase (amdS) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 5 sequence (CNAG_05670 - CNAG_05671) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:46 | 0 | |
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pPEE28 Resource Report Resource Website |
RRID:Addgene_128368 | Noureothricin (NAT) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 4 sequence (CNAG_06526 - CNAG_06527) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | 2026-08-15 01:04:46 | 0 | |
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S9_attL1-EGFP-C-term-attL2 Resource Report Resource Website |
RRID:Addgene_128473 | EGFP entry clone | Synthetic | Kanamycin | PMID:31959800 | Backbone Marker:Genewiz; Backbone Size:2579; Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:04:47 | 0 | ||
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S13_EcoRV_UBC_AscI Resource Report Resource Website |
RRID:Addgene_128477 | UBC promoter | Synthetic | Kanamycin | PMID:31959800 | Backbone Marker:Genewiz; Backbone Size:2579; Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:04:48 | 0 | ||
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S12_EcoRV_PGK_AscI Resource Report Resource Website |
RRID:Addgene_128476 | PGK promoter | Synthetic | Kanamycin | PMID:31959800 | Backbone Marker:Genewiz; Backbone Size:2579; Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:04:47 | 0 | ||
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S7_SwaI_Zeo_PacI Resource Report Resource Website |
RRID:Addgene_128471 | Zeocin Resistance gene | Synthetic | Kanamycin | PMID:31959800 | Backbone Marker:Genewiz; Backbone Size:2579; Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:04:47 | 0 | ||
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S6_BstBI_SV40_SwaI Resource Report Resource Website |
RRID:Addgene_128470 | SV40 promoter | Synthetic | Kanamycin | PMID:31959800 | Backbone Marker:Genewiz; Backbone Size:2579; Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:04:47 | 0 | ||
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pSMPUW_8.4_Ex_CAMKIIA-EGFP-V5-SV40-Zeo Resource Report Resource Website |
RRID:Addgene_128464 | CAMKIIA promoter, EGFP gene, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene | Synthetic | Kanamycin | Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Kanamycin | 2026-08-15 01:04:47 | 0 | |||
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pSMPUW_8.1_ M2_CAMKIIA-gateway-V5 Resource Report Resource Website |
RRID:Addgene_128461 | CAMKIIA promoter and C-terminal V5 tag | Synthetic | Chloramphenicol and Kanamycin | Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Kanamycin | 2026-08-15 01:04:47 | 0 | |||
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S5_EcoRV_Ef1a_AscI Resource Report Resource Website |
RRID:Addgene_128469 | Ef1a promoter | Synthetic | Kanamycin | PMID:31959800 | Backbone Marker:Genewiz; Backbone Size:2579; Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:04:47 | 0 | ||
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pSMPUW_6.19_Ex_Ef1a-EGFP-HA-SV40-Zeo Resource Report Resource Website |
RRID:Addgene_128455 | Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeocin Resistance gene | Synthetic | Kanamycin | Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Kanamycin | 2026-08-15 01:04:47 | 0 | |||
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pSMPUW_6.4_M1-gateway-V5 Resource Report Resource Website |
RRID:Addgene_128440 | C-terminal V5 tag | Synthetic | Chloramphenicol and Kanamycin | Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Kanamycin | 2026-08-15 01:04:47 | 0 |
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