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Species: Synthetic
Genetic Insert: Neomycin (NEO)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 7 sequence (CNAG_05557 - CNAG_05558) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128376 Copy
Species: Synthetic
Genetic Insert: Neomycin (NEO)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 6 sequence (CNAG_04586 - CNAG_04585) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128375 Copy
Species: Synthetic
Genetic Insert: Neomycin (NEO)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 5 sequence (CNAG_05670 - CNAG_05671) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128374 Copy
Species: Synthetic
Genetic Insert: Hygromycin (HYG)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 5 sequence (CNAG_05670 - CNAG_05671) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128379 Copy
Species: Synthetic
Genetic Insert: Hygromycin (HYG)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 4 sequence (CNAG_06526 - CNAG_06527) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128378 Copy
Species: Synthetic
Genetic Insert: Hygromycin (HYG)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 3 sequence (CNAG_02589 - CNAG_02588) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128377 Copy
Species: Synthetic
Genetic Insert: gfpmut2
Vector Backbone Description: Vector Backbone:pLG166; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31792444
Proper citation: RRID:Addgene_128410 Copy
Species: Synthetic
Genetic Insert: Neomycin (NEO)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 3 sequence (CNAG_02589 - CNAG_02588) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128372 Copy
Species: Synthetic
Genetic Insert: Acetamidase (amdS)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 5 sequence (CNAG_05670 - CNAG_05671) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128364 Copy
Species: Synthetic
Genetic Insert: Noureothricin (NAT)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 4 sequence (CNAG_06526 - CNAG_06527) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128368 Copy
Species: Synthetic
Genetic Insert: EGFP entry clone
Vector Backbone Description: Backbone Marker:Genewiz; Backbone Size:2579; Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31959800
Proper citation: RRID:Addgene_128473 Copy
Species: Synthetic
Genetic Insert: UBC promoter
Vector Backbone Description: Backbone Marker:Genewiz; Backbone Size:2579; Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31959800
Proper citation: RRID:Addgene_128477 Copy
Species: Synthetic
Genetic Insert: PGK promoter
Vector Backbone Description: Backbone Marker:Genewiz; Backbone Size:2579; Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31959800
Proper citation: RRID:Addgene_128476 Copy
Species: Synthetic
Genetic Insert: Zeocin Resistance gene
Vector Backbone Description: Backbone Marker:Genewiz; Backbone Size:2579; Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31959800
Proper citation: RRID:Addgene_128471 Copy
Species: Synthetic
Genetic Insert: SV40 promoter
Vector Backbone Description: Backbone Marker:Genewiz; Backbone Size:2579; Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31959800
Proper citation: RRID:Addgene_128470 Copy
Species: Synthetic
Genetic Insert: CAMKIIA promoter, EGFP gene, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_128464 Copy
Species: Synthetic
Genetic Insert: CAMKIIA promoter and C-terminal V5 tag
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_128461 Copy
Species: Synthetic
Genetic Insert: Ef1a promoter
Vector Backbone Description: Backbone Marker:Genewiz; Backbone Size:2579; Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31959800
Proper citation: RRID:Addgene_128469 Copy
Species: Synthetic
Genetic Insert: Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_128455 Copy
Species: Synthetic
Genetic Insert: C-terminal V5 tag
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_128440 Copy
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