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Species: Homo sapiens
Genetic Insert: HS4-TRE-cit-HS4-mch (DH)
Vector Backbone Description: Vector Backbone:pAAVS1 donor ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35678392
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.04.467237v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_179436 Copy
Species: Homo sapiens
Genetic Insert: coreHS4-TRE-cit-coreHS4-mch (DC)
Vector Backbone Description: Vector Backbone:pAAVS1 donor ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35678392
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.04.467237v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_179437 Copy
Species: Homo sapiens
Genetic Insert: ELMSAN1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5367; Vector Backbone:pET28C (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34912035
Comments: The insert encodes the epitope (ELMSAN1 residue 550-600) of the antibody (Catalog # A303-157A, BETHYL). Expressed protein has been confirmed by Western blot.
Proper citation: RRID:Addgene_179391 Copy
Species: Homo sapiens
Genetic Insert: DDX21
Vector Backbone Description: Vector Backbone:pHAGE-EF1a-IRES-ZsGreen; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34326237
Proper citation: RRID:Addgene_179229 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting human PER2
Vector Backbone Description: Backbone Marker:Zhang lab, addgene #52961; Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34145278
Comments: Designed to be used with Addgene #179448-179452
Proper citation: RRID:Addgene_179456 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting human PER2
Vector Backbone Description: Backbone Marker:Zhang lab, addgene #52961; Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34145278
Comments: Designed to be used with Addgene #179448-179452
Proper citation: RRID:Addgene_179454 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting human PER2
Vector Backbone Description: Backbone Marker:Zhang lab, addgene #52961; Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34145278
Comments: Designed to be used with Addgene #179448-179452
Proper citation: RRID:Addgene_179455 Copy
Species: Homo sapiens
Genetic Insert: G0S2
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:5676; Vector Backbone:pMAL-c5E; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34260600
Comments: Protein sequence is the mature maltose binding protein (identical to aa 1-367 of accession no. 6K7E_A) + NSSSNNNNNNNNNNLG + enterokinase cleavage site (DDDDK*; * = cleavage site) + VPH + human G0S2 (G0/G1 switch protein 2; accession number NP_056529.1) + L.
Confers ampicillin-resistance.
This plasmid has been referred to as MBP g0s2, MBP-g0s2, g0s2-noHis_pMAL-c5E, or pHis6-MBP-g0s2. There is no His-tag in the expressed protein sequence.
Backbone vector is pMAL-c5E.
Construct design by James Zook.
Publication: Moran et al 2021 PLoS One 16, e0249164, https://doi.org/10.1371/journal.pone.0249164
Proper citation: RRID:Addgene_179294 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting human CRY1
Vector Backbone Description: Backbone Marker:Zhang lab, addgene #52961; Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34145278
Comments: Designed to be used with Addgene #179441-179447
Proper citation: RRID:Addgene_179453 Copy
Species: Homo sapiens
Genetic Insert: NDFIP2
Vector Backbone Description: Backbone Marker:GEHealthcare; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_179330 Copy
Species: Homo sapiens
Genetic Insert: FBL
Vector Backbone Description: Vector Backbone:PGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34326237
Proper citation: RRID:Addgene_179245 Copy
Species: Homo sapiens
Genetic Insert: B55δ
Vector Backbone Description: Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21159657
Proper citation: RRID:Addgene_179355 Copy
Species: Homo sapiens
Genetic Insert: DDX21
Vector Backbone Description: Vector Backbone:pHAGE-EF1a-IRES-ZsGreen; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34326237
Proper citation: RRID:Addgene_179233 Copy
Species: Homo sapiens
Genetic Insert: B56α
Vector Backbone Description: Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21159657
Proper citation: RRID:Addgene_179352 Copy
Species: Homo sapiens
Genetic Insert: TRE-cit-5kb-mch
Vector Backbone Description: Backbone Marker:Yamaguchi et al. 2011 PLoS One, doi: 10.1371/journal.pone.0017267; Vector Backbone:pSLR-test; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35678392
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.04.467237v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_179426 Copy
Species: Homo sapiens
Genetic Insert: EGFR
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34965432
Proper citation: RRID:Addgene_179262 Copy
Species: Homo sapiens
Genetic Insert: EGFR
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34965432
Proper citation: RRID:Addgene_179263 Copy
Species: Homo sapiens
Genetic Insert: B56δ
Vector Backbone Description: Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21159657
Proper citation: RRID:Addgene_179371 Copy
Species: Homo sapiens
Genetic Insert: FRB
Vector Backbone Description: Vector Backbone:CMVTO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35177637
Proper citation: RRID:Addgene_179676 Copy
Species: Homo sapiens
Genetic Insert: NLS-NG-T2A-hSHH
Vector Backbone Description: Vector Backbone:KS(-); Vector Types:piggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34799555
Proper citation: RRID:Addgene_179586 Copy
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