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Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2
Vector Backbone Description: Backbone Size:5385; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21796121
Comments: ChR2 variant harboring two amino acid substitutions which act to stabilize the conducting state of the channel to deactivate with a time constant of nearly 30 minutes following a brief pulse of activating blue light. Like previously published step function opsins, this stabilized step function opsin (SSFO) may be deactivated using yellow light (590nm). The stabilized open state of the channel allows for both lower power activation, meaning in some circumstances the light delivery system need not penetrate the brain, as well as for behavior in the absence of a tethered laser or other light delivery system.
Proper citation: RRID:Addgene_35501 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5797; Vector Backbone:pRS32x; Vector Types:Yeast 2 micron vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: HIS3 has been modified to remove HindIII, KpnI, and PstI sites without altering the amino acid sequence of the His3 protein.
Proper citation: RRID:Addgene_35463 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5605; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22179551
Comments:
Proper citation: RRID:Addgene_35508 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5605; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22179551
Proper citation: RRID:Addgene_35507 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5605; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22179551
Proper citation: RRID:Addgene_35509 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4886; Vector Backbone:pRS41x; Vector Types:Yeast centromere vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: URA3 has been modified to remove ApaI and PstI sites without altering the amino acid sequence of the Ura3 protein.
Proper citation: RRID:Addgene_35456 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6009; Vector Backbone:pRS41x; Vector Types:Yeast centromere vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: LEU2 has been modified to remove ClaI, EcoRI and KpnI sites without altering the amino acid sequence of the Leu2 protein.
Proper citation: RRID:Addgene_35454 Copy
Species: Homo sapiens
Genetic Insert: MHC IIA
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17567956
Proper citation: RRID:Addgene_35687 Copy
Species: Homo sapiens
Genetic Insert: MHC IIA
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18971378
Proper citation: RRID:Addgene_35689 Copy
Species: Homo sapiens
Genetic Insert: Estrogen Receptor beta
Vector Backbone Description: Vector Backbone:pcDNA 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35562 Copy
Species: Homo sapiens
Genetic Insert: ERbeta
Vector Backbone Description: Vector Backbone:pSUPER puro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19106194
Proper citation: RRID:Addgene_35561 Copy
Species: Homo sapiens
Genetic Insert: MRLC1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22136066
Comments: This plasmid is identical to the one published in Beach et al., 2011, except the tet-inducible promoter has been replaced with a constitutive CMV promoter.
Proper citation: RRID:Addgene_35681 Copy
Species: Homo sapiens
Genetic Insert: MRLC1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22136066
Comments: This plasmid is identical to the one published in Beach et al., 2011, except the tet-inducible promoter has been replaced with a constitutive CMV promoter.
Proper citation: RRID:Addgene_35680 Copy
Genetic Insert: none
Vector Backbone Description: Vector Backbone:NA; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:10610690
Comments: To be used with the following plasmids from the Matthews lab: pTARA (www.addgene.org/31491), pLS1 (www.addgene.org/31490), and (www.addgene.org/31492) pLS1/-11
Proper citation: RRID:Addgene_35609 Copy
Species: Mus musculus
Genetic Insert: Synectin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16908842
Comments: This construct contains a K111R mutation when compared to GenBank ID NP_061241.1 This mutation is most likely a sequence conflict, one of several (see Uniprot entry Q9Z0G0). The plasmid is exactly as described in the associated publication.
Proper citation: RRID:Addgene_35791 Copy
Species: Synthetic
Genetic Insert: U6 let-7sponge
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:5124; Vector Backbone:pRNA-U6.1/Neo-siFluc; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388288
Proper citation: RRID:Addgene_35664 Copy
Species: E. coli
Genetic Insert: BirA
Vector Backbone Description: Backbone Marker:InVitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22412018
Proper citation: RRID:Addgene_35700 Copy
Species: Mus musculus
Genetic Insert: FoxO1-ADA-GFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20519497
Comments: E15G and G282R mutations are present in this insert but are not known to affect function. There are also K219R and L619P polymorphisms. Plasmid should still function as expected of the ADA.
Proper citation: RRID:Addgene_35640 Copy
Species: Homo sapiens
Genetic Insert: N-Ras(G12V)
Vector Backbone Description: Backbone Marker:Eric Campeau; Backbone Size:10000; Vector Backbone:pLenti-PGK-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22341439
Proper citation: RRID:Addgene_35632 Copy
Species: Homo sapiens
Genetic Insert: K-Ras4A(G12V)
Vector Backbone Description: Backbone Marker:Eric Campeau; Backbone Size:10000; Vector Backbone:pLenti-PGK-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22341439
Proper citation: RRID:Addgene_35634 Copy
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