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Species: Saccharomyces cerevisiae
Genetic Insert: ATG13
Vector Backbone Description: Vector Backbone:pRS315; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077553
Proper citation: RRID:Addgene_59544 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: loxP-HphMX-loxP-3FLAG
Vector Backbone Description: Backbone Marker:Euroscarf; Vector Backbone:pUG6; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27943405
Proper citation: RRID:Addgene_85780 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: loxP-HphMX-loxP-PYK1p-3FLAG
Vector Backbone Description: Backbone Marker:Euroscarf; Vector Backbone:pUG6; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27943405
Proper citation: RRID:Addgene_85781 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: loxP-HphMX-loxP-GAL1p-3FLAG
Vector Backbone Description: Backbone Marker:Euroscarf; Vector Backbone:pUG6; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27943405
Proper citation: RRID:Addgene_85782 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: loxP-NatMX-loxP-CYC1p-3FLAG
Vector Backbone Description: Backbone Marker:Euroscarf; Vector Backbone:pUG6; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27943405
Comments: *Note: Addgene's quality control sequencing finds a P77L amino acid residue substitution in the Nourseothricin resistance gene, but this residue change is not thought to affect antibiotic selection.
Proper citation: RRID:Addgene_85783 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: loxP-KanMX-loxP-GAL1p-3FLAG
Vector Backbone Description: Backbone Marker:Euroscarf; Vector Backbone:pUG6; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27943405
Proper citation: RRID:Addgene_85778 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: loxP-KanMX-loxP-3FLAG
Vector Backbone Description: Backbone Marker:Euroscarf; Vector Backbone:pUG6; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27943405
Proper citation: RRID:Addgene_85776 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: loxP-KanMX-loxP-PYK1p-3FLAG
Vector Backbone Description: Backbone Marker:Euroscarf; Vector Backbone:pUG6; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27943405
Proper citation: RRID:Addgene_85771 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: loxP-KanMX-loxP-GAL1p-3FLAG
Vector Backbone Description: Backbone Marker:Euroscarf; Vector Backbone:pUG6; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27943405
Proper citation: RRID:Addgene_85772 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUA7
Vector Backbone Description: Backbone Size:5741; Vector Backbone:YEplac181; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15082542
Proper citation: RRID:Addgene_8627 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RTS1
Vector Backbone Description: Backbone Size:5741; Vector Backbone:YEplac181; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15082542
Proper citation: RRID:Addgene_8626 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: DST1
Vector Backbone Description: Backbone Size:5741; Vector Backbone:YEplac181; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15082542
Proper citation: RRID:Addgene_8624 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TYS1
Vector Backbone Description: Backbone Size:5741; Vector Backbone:YEplac181; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15082542
Proper citation: RRID:Addgene_8629 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SOH1
Vector Backbone Description: Backbone Size:6021; Vector Backbone:pRS415; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15082542
Proper citation: RRID:Addgene_8622 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Nop1pr-GFP11-mCherry-PUS1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27831485
Proper citation: RRID:Addgene_86413 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HHT2
Vector Backbone Description: Backbone Marker:Akash Gunjan, Addgene Plasmid 86470; Vector Backbone:pYES2-HTH; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19578373
Proper citation: RRID:Addgene_86482 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mTurquoise2
Vector Backbone Description: Backbone Size:4200; Vector Backbone:pFA6-link-CaURA3MX; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26371506
Comments: use F5/R3 primers
Proper citation: RRID:Addgene_86424 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1
Vector Backbone Description: Backbone Marker:Kuo et al, Mol Cell Biol. 1983 October; 3(10): 1730; Backbone Size:0; Vector Backbone:YCp50; Vector Types:Yeast Expression, Centromeric; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3540600
Comments: The TUB1 centromere plasmid was constructed by ligating a TUB1 fragment from the SphI site (1.1 kilobases before the start codon) to a BglII site (0.5 kb beyond the stop codon) in place of the small SphI-to-BamHI fragment of YCp50. For more information on YCp50, including map, search for YCp50 in Addgene's vector db.
Proper citation: RRID:Addgene_8667 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1
Vector Backbone Description: Backbone Marker:Bolivar et al., Gene. 1977;2(2):95-113.; Backbone Size:0; Vector Backbone:pBR322; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3540600
Comments: For more information on pBR322, including a map, search for pBR322 in Addgene's vector db.
Proper citation: RRID:Addgene_8666 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1 upstream of LEU2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pUC119; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10793159
Comments: TUB1 fragment (SphI-SacI) from pRB306 inserted into SphI-SacI-cut polylinker of pUC119.
Then, BamHI-SalI fragment from pJJ282 (LEU2 gene) Klenow filled and inserted into the HpaI site, downstream of TUB1; LEU2 is oriented in the same direction as TUB1. For information on pUC119, search for pUC119 in Addgene's vector db.
Proper citation: RRID:Addgene_8669 Copy
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