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Species: Homo sapiens
Genetic Insert: SUMO1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Comments: Please note this plasmid contains the mature form of SUMO1, which lacks the last 4 amino acids from the C-terminus.
Proper citation: RRID:Addgene_48966 Copy
Species: Homo sapiens
Genetic Insert: SUMO1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Proper citation: RRID:Addgene_48965 Copy
Genetic Insert: cas9
Vector Backbone Description: Backbone Marker:life technologies; Backbone Size:4959; Vector Backbone:pDEST; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24013562
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_48960 Copy
Species: Homo sapiens
Genetic Insert: dCas9_VP64 (human-codon-optimized)
Vector Backbone Description: Backbone Marker:Evrogen; Backbone Size:4000; Vector Backbone:phi-Yellow-Dest; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Comments: L591M mutation with dCas9
Proper citation: RRID:Addgene_49015 Copy
Species: Homo sapiens
Genetic Insert: Malic Enzyme 1
Vector Backbone Description: Backbone Marker:Addgene plasmid # 1764; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23334421
Proper citation: RRID:Addgene_49163 Copy
Species: Enterobacteria phage T4
Genetic Insert: Bacteriophage T4 gene 600
Vector Backbone Description: Backbone Size:2662; Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23492219
Proper citation: RRID:Addgene_49159 Copy
Species: Synthetic
Genetic Insert: ssAAV-smCBA-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49142 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple155-emGFP-WPRE
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531684
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Additional References:
1 Korecki, A. J. et al. Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain, Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. Genetics 211, 1155-1177 (2019).
2 de Leeuw, C. N. et al. rAAV-Compatible MiniPromoters for Restricted Expression in the Brain and Eye. Molecular Brain 9, 52 (2016).
3 Scalabrino, M. L. et al. Intravitreal delivery of a novel AAV vector targets ON bipolar cells and restores visual function in a mouse model of complete congenital stationary night blindness. Hum Mol Genet 24, 6229-6239, doi:10.1093/hmg/ddv341, ddv341 [pii] (2015).
4 de Leeuw, C. N. et al. Targeted CNS delivery using human MiniPromoters and demonstrated compatibility with adeno-associated viral vectors. Mol Ther Methods Clin Dev 1, 1-15 (2014).
Proper citation: RRID:Addgene_49140 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple67-emGFP-WPRE
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30765420
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49138 Copy
Species: Mus musculus
Genetic Insert: Smo
Vector Backbone Description: Backbone Size:6833; Vector Backbone:pEF5B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23930224
Proper citation: RRID:Addgene_49099 Copy
Species: Synthetic
Genetic Insert: ssAAV-hs1218-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Additional reference for hs1218 promoter: Visel et al., 2007. VISTA Enhancer Browser--a database of tissue-specific human enhancers. Nucleic Acids Res. 2007 Jan;35(Database issue):D88-92. Epub 2006 Nov 27. PMID: 17130149 (https://www.ncbi.nlm.nih.gov/pubmed/17130149)
Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49130 Copy
Species: Danio rerio
Genetic Insert: Etv5 S142 D
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCs2+; Vector Types:Mammalian Expression, xenopus/avian/zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20346941
Proper citation: RRID:Addgene_49096 Copy
Species: Synthetic
Genetic Insert: ssAAV-CAGGS-emGFP-WPRE
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49136 Copy
Species: Synthetic
Genetic Insert: ssAAV-CAGGS-emGFP
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49135 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple305-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49134 Copy
Species: Synthetic
Genetic Insert: ssAAV-PRS2/3:Con-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: For additional information, please see the following publications:
Hwang DY et al., 2005. Genetically engineered dopamine beta-hydroxylase gene promoters with better phox2-binding sites drive significantly enhanced transgene expression in a noradrenergic cell-specific manner. Mol Ther 11, 132-141, PMID 15585414 (https://www.ncbi.nlm.nih.gov/pubmed/15585414).
de Leeuw CN et al., rAAV-compatible MiniPromoters for restricted expression in the brain and eye. Mol Brain. 2016 May 10;9(1):52, PMID 27164903 (https://www.ncbi.nlm.nih.gov/pubmed/27164903).
Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49129 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple267-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49126 Copy
Species: Synthetic
Genetic Insert: ssAAV-CAGGS-iCre-WPRE
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49120 Copy
Species: Homo sapiens
Genetic Insert: human NKCC1 (synthetic)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5316; Vector Backbone:pcDNA3.1 neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24339991
Comments: Synthetic cDNA encoding full length human NKCC1, with 29 silent restriction sites in NKCC1 coding region
This plasmid has also been described in Monette et al., J Biol Chem. 2014 Jan 22 (https://www.ncbi.nlm.nih.gov/pubmed/24451383)
Proper citation: RRID:Addgene_49085 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple94-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49125 Copy
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