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Species: Saccharomyces cerevisiae
Genetic Insert: TUB1-E255A under GAL1 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pTS210; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11739794
Comments: PCRed TUB1-E255A products were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS210. The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.
Proper citation: RRID:Addgene_8674 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1 with LYS2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pUC119; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10793159
Comments: TUB1 fragment (SphI-SacI) from pRB306 inserted into SphI-SacI-cut polylinker of pUC119. Then, PvuII fragment from pRS317 containing LYS2 gene inserted into HpaI site; LYS2 is oriented in the same direction as TUB1. For more information on pUC119, search for pUC119 in Addgene's vector db.
Proper citation: RRID:Addgene_8671 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GFP::TUB1 under GAL1 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pTS408; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11739794
Comments: TUB1 wildtype DNA were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS408. pTS408 was constructed by PCR amplifying GFP with BamHI and BglII sites and by inserting this into the BamHI site of pTS210, creating a plasmid with the polylinker downstream of GFP to create NH2-terminal GFP fusions.The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.
Proper citation: RRID:Addgene_8677 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TEF1 promoter-yEGFP-ERG9 ER signal peptide
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27939849
Proper citation: RRID:Addgene_83561 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TEF1 promoter -yEGFP-CLN2PEST
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26112740
Proper citation: RRID:Addgene_83560 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27939849
Proper citation: RRID:Addgene_83562 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH2 promoter-yEGFP
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26112740
Comments: Please note that the promoters were cloned from strain CEN.PK113-7D and the assembled full sequence are based on S288C promoters. Discrepancies in these promoters are the SNPs in CEN.PK113-7D.
Proper citation: RRID:Addgene_83554 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CUP1 promoter-yEGFP
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26112740
Comments: Please note that the promoters were cloned from strain CEN.PK113-7D and the assembled full sequence are based on S288C promoters. Discrepancies in these promoters are the SNPs in CEN.PK113-7D.
Proper citation: RRID:Addgene_83555 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL7 promoter-yEGFP
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28239415
Comments: Please note that the promoters were cloned from strain CEN.PK113-7D and the assembled full sequence are based on S288C promoters. Discrepancies in these promoters are the SNPs in CEN.PK113-7D.
Proper citation: RRID:Addgene_83559 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA targeting ADE2 gene
Vector Backbone Description: Backbone Marker:George Church lab, Dicarlo et al Nucleic Acids Res; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34150504
Proper citation: RRID:Addgene_83947 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MATa1(78aa-126aa)
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6700; Vector Backbone:modified pMal-c2x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12538894
Proper citation: RRID:Addgene_8436 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAP1
Vector Backbone Description: Vector Backbone:pAG415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25800674
Proper citation: RRID:Addgene_84533 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAP1
Vector Backbone Description: Vector Backbone:pAG415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25800674
Comments: Mutation numbering may differ from listed mutations, but depositor confirms that the correct mutations are present within the plasmid.
Proper citation: RRID:Addgene_84535 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAP1
Vector Backbone Description: Vector Backbone:pAG415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25800674
Proper citation: RRID:Addgene_84536 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAP1
Vector Backbone Description: Vector Backbone:pAG415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25800674
Comments: Mutation numbering may differ from listed mutations, but depositor confirms that the correct mutations are present within the plasmid.
Proper citation: RRID:Addgene_84531 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAP1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway donor plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25800674
Proper citation: RRID:Addgene_84528 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAP1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway donor plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25800674
Proper citation: RRID:Addgene_84564 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ERKsub(TA)DD-Venus-NES
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26548610
Proper citation: RRID:Addgene_84631 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26548610
Proper citation: RRID:Addgene_84637 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cerulean-WW-NES
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26548610
Proper citation: RRID:Addgene_84629 Copy
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