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Species: Synthetic
Genetic Insert: dCas9-hudTET1CD, SgRNA cloning site
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458 ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33488615
Comments: For SgRNA cloning: Caution! BbsI cannot be used for SgRNA cloning in this plasmid, there are BbsI sites in TET1CD. Instead the guide sequence can be cloned into Addgene#48138 and in a second step transferred into this plasmid (for example using PvuI & XbaI).
This plasmid was generated by Christopher Kressler.
Proper citation: RRID:Addgene_129026 Copy
Species: Synthetic
Genetic Insert: dCas9-huTET1CD, SgRNA cloning site
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458 ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33488615
Comments: For SgRNA cloning: Caution! BbsI cannot be used for SgRNA cloning in this plasmid, there are BbsI sites in TET1CD. Instead the guide sequence can be cloned into Addgene#48138 and in a second step transferred into this plasmid (for example using PvuI & XbaI).
This plasmid was generated by Christopher Kressler.
Proper citation: RRID:Addgene_129027 Copy
Species: Synthetic
Genetic Insert: dCas9-huTET1CD, SgRNA cloning site
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458 ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33488615
Comments: For SgRNA cloning: Caution! BbsI cannot be used for SgRNA cloning in this plasmid, there are BbsI sites in TET1CD. Instead the guide sequence can be cloned into Addgene#48138 and in a second step transferred into this plasmid (for example using PvuI & XbaI).
This Plasmid was generated by Christopher Kressler.
Proper citation: RRID:Addgene_129025 Copy
Species: Synthetic
Genetic Insert: co frp
Vector Backbone Description: Backbone Size:5344; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31792180
Proper citation: RRID:Addgene_129090 Copy
Species: Synthetic
Genetic Insert: TEF1p-SS-3xGlyAla-GFP-HDEL
Vector Backbone Description: Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30084355
Comments: Linearize the plasmid with StuI for integration into URA3 locus.
Proper citation: RRID:Addgene_129098 Copy
Species: Synthetic
Genetic Insert: co luxD
Vector Backbone Description: Backbone Size:5344; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31792180
Proper citation: RRID:Addgene_129088 Copy
Species: Synthetic
Genetic Insert: QUEEN-37C
Vector Backbone Description: Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Depositor Adm #1081 . Please visit https://www.biorxiv.org/content/10.1101/2021.10.08.463131v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_129323 Copy
Species: Synthetic
Genetic Insert: pHluorin (from H. Imamura)
Vector Backbone Description: Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Depositor Adm #1067 . Please visit https://www.biorxiv.org/content/10.1101/2021.10.08.463131v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_129320 Copy
Species: Synthetic
Genetic Insert: QUEEN-37C
Vector Backbone Description: Vector Backbone:pLBS.CAG; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Depositor Adm #1080 . Please visit https://www.biorxiv.org/content/10.1101/2021.10.08.463131v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_129322 Copy
Species: Synthetic
Genetic Insert: QUEEN-BPEK
Vector Backbone Description: Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Depositor Adm #1030 . Please visit https://www.biorxiv.org/content/10.1101/2021.10.08.463131v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_129313 Copy
Species: Synthetic
Genetic Insert: QUEEN-7mu
Vector Backbone Description: Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25283467
Comments: Depositor Adm #1031
Proper citation: RRID:Addgene_129314 Copy
Species: Synthetic
Genetic Insert: pHluorin (from H. Imamura)
Vector Backbone Description: Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Depositor Adm #1033 . Please visit https://www.biorxiv.org/content/10.1101/2021.10.08.463131v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_129315 Copy
Species: Synthetic
Genetic Insert: QUEEN-7mu
Vector Backbone Description: Vector Backbone:pRSET B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25283467
Comments: Depositor Adm #1028
Proper citation: RRID:Addgene_129311 Copy
Species: Synthetic
Genetic Insert: QUEEN-7mu R122A
Vector Backbone Description: Vector Backbone:pRSET B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Depositor Adm #1041 . Please visit https://www.biorxiv.org/content/10.1101/2021.10.08.463131v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_129317 Copy
Species: Synthetic
Genetic Insert: QUEEN-37C
Vector Backbone Description: Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Depositor Adm #1042 . Please visit https://www.biorxiv.org/content/10.1101/2021.10.08.463131v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_129318 Copy
Species: Synthetic
Genetic Insert: NLS-VP16-ZF 43-8-NES
Vector Backbone Description: Backbone Marker:Gregory Newby; Vector Backbone:pGAN147; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29056345
Comments: This is the zinc finger only control for the yTRAP sensor plasmid. Linearize with NotI-HF enzyme prior to integration into the yeast genome. pSK267 integrates into the HO locus, and can be selected for using nourseothricin, it constitutively produces high mNeonGreen reporter output.
Proper citation: RRID:Addgene_129272 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Gregory Newby; Vector Backbone:pGAN147; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29056345
Comments: reporter only (no yTRAP fusion). 8xop-crCYC1p-NeonGreen-ADH1term.
for measuring background levels of reporter protein
Proper citation: RRID:Addgene_129270 Copy
Species: Synthetic
Genetic Insert: QUEEN-NA
Vector Backbone Description: Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25283467
Comments: Depositor Adm #1026
Proper citation: RRID:Addgene_129309 Copy
Species: Synthetic
Genetic Insert: QUEEN-7mu
Vector Backbone Description: Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25283467
Comments: Depositor Adm #1024
Proper citation: RRID:Addgene_129307 Copy
Species: Synthetic
Genetic Insert: QUEEN-2m
Vector Backbone Description: Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25283467
Comments: Depositor Adm #1025
Proper citation: RRID:Addgene_129308 Copy
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