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Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180329 Copy
Species: Homo sapiens
Genetic Insert: HLA-B*35:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00237 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180450 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*03:01
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEMEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00037 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180451 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*02:01 (CD8 mut)
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Mutation prevents binding of CD8 (Purbhoo et al. (2001) J. Biol. Chem. doi.org/10.1074/jbc.M102498200). See https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00005 for allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180459 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180334 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180339 Copy
Species: Homo sapiens
Genetic Insert: RPS6KA2
Vector Backbone Description: Backbone Size:8015; Vector Backbone:W118-1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33709453
Proper citation: RRID:Addgene_180384 Copy
Species: Homo sapiens
Genetic Insert: RPS6KA2_K91A/K444A
Vector Backbone Description: Backbone Size:8015; Vector Backbone:W118-1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33709453
Proper citation: RRID:Addgene_180385 Copy
Species: Homo sapiens
Genetic Insert: ZBTB7B
Vector Backbone Description: Backbone Size:8015; Vector Backbone:W118-1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33709453
Proper citation: RRID:Addgene_180380 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN7
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34854883
Comments: The mutant is the fusion of the SEPT9_i1-specific N-terminal sequence to SEPT9_i5.
Please note: Plasmid contains a 65bp insertion in the CloDF13 ori. This insertion is not known to affect plasmid function.
Proper citation: RRID:Addgene_180314 Copy
Species: Homo sapiens
Genetic Insert: human Otubain-1
Vector Backbone Description: Backbone Marker:TAKARA BIO; Backbone Size:5934; Vector Backbone:pRetroX-IRES-DsRedExpress Vector; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35159073
Comments: The backbone vector's cat.#632521 from TAKARA BIO
Proper citation: RRID:Addgene_180279 Copy
Species: Homo sapiens
Genetic Insert: Progranulin
Vector Backbone Description: Backbone Marker:Franck Perez (Addgene plasmid # 65295); Vector Backbone:Str-KDEL_SBP-mCherry-GPI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34919127
Proper citation: RRID:Addgene_180439 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180319 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180340 Copy
Species: Homo sapiens
Genetic Insert: HLA-B*40:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00291 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180463 Copy
Species: Homo sapiens
Genetic Insert: SENP2
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20417598
Comments: Note that compared to GenBank reference sequence NP_067640.2, the SENP2 insert in this plasmid contains a T552A polymorphism.
Proper citation: RRID:Addgene_18047 Copy
Species: Homo sapiens
Genetic Insert: TRBP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29939295
Proper citation: RRID:Addgene_180600 Copy
Species: Homo sapiens
Genetic Insert: TRBP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29939295
Proper citation: RRID:Addgene_180602 Copy
Species: Homo sapiens
Genetic Insert: ddGFP sensor of Kras
Vector Backbone Description: Vector Backbone:pCMV-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30643148
Proper citation: RRID:Addgene_180571 Copy
Species: Homo sapiens
Genetic Insert: ddRFP sensor of Kras
Vector Backbone Description: Vector Backbone:pCMV-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30643148
Proper citation: RRID:Addgene_180572 Copy
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