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Species: E. coli
Genetic Insert: BirA(R118G)-HA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5333; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22412018
Proper citation: RRID:Addgene_36047 Copy
Species: Homo sapiens
Genetic Insert: alpha-synuclein
Vector Backbone Description: Backbone Size:2438; Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18343814
Comments: Lashuel Lab Plasmid #77
Proper citation: RRID:Addgene_36046 Copy
Species: Rattus norvegicus
Genetic Insert: G protein subunit Gamma 5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16517125
Proper citation: RRID:Addgene_36044 Copy
Species: Rattus norvegicus
Genetic Insert: protein tyrosine phosphatase 1B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18332219
Comments: A Kozak methionine initiator sequence and a Flag tag were added to the N terminus of rat PTP1B, which was amplified by PCR with the following primers: 5′-CTCGGATCCGCCACCATGGACTACAAGGACGACGATGACAAGAAGCTTGGGATGGAAATGGAGAAGGAATTCGAG-3′ and 5′-CTCTAGACTCGAGCGGCCGCTCAGTGAAAACATACCCGGTAACAG-3′. The PCR product was digested with BamHI and NotI and subsequently ligated into the corresponding sites of pCDNA3.1(+) (Invitrogen)
Please note that Addgene's sequencing results differ from the full plasmid sequence information from the depositing laboratory at bp# 889-910, indicating that the NheI and AflII sites are no longer present in the plasmid.
Proper citation: RRID:Addgene_35988 Copy
Species: Homo sapiens
Genetic Insert: CHOP promoter (-647 to +136)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4140; Vector Backbone:pmCherry-1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22215663
Proper citation: RRID:Addgene_36035 Copy
Vector Backbone Description: Backbone Size:9100; Vector Backbone:pDRf1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17293878
Proper citation: RRID:Addgene_36026 Copy
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pDR195; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16407306
Proper citation: RRID:Addgene_36029 Copy
Vector Backbone Description: Backbone Marker:Stover et al., 1991 Nature 351:456; Vector Backbone:pMV261; Vector Types:Bacterial Expression, Synthetic Biology, Mycobacteria replicating vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22279533
Comments: The plasmid is high copy in E. coli, but low copy in mycobacteria.
Proper citation: RRID:Addgene_36251 Copy
Species: Homo sapiens
Genetic Insert: WASp
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:6857; Vector Backbone:pRRLSIN.cppt.PGK-GFP.WPRE Addgene 12252; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22431569
Proper citation: RRID:Addgene_36250 Copy
Genetic Insert: Two copies of the Brachyury T-box site
Vector Backbone Description: Backbone Size:6800; Vector Backbone:pTK-luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22354841
Comments: Brachyury palindromic element:
AATTTCACACCTAGGTGTGAAATT
Proper citation: RRID:Addgene_36246 Copy
Genetic Insert: Two copies of the Brachyury T-box site
Vector Backbone Description: Backbone Size:6800; Vector Backbone:pTK-luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22354841
Comments: Mutated Brachyury palindromic element:
AATTTGGGACCTAGGTCCCAAATT
Proper citation: RRID:Addgene_36245 Copy
Species: Homo sapiens
Genetic Insert: DOT1L (GeneScript Synthesized)
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3SX0. http://www.thesgc.org/structures/3SX0/
PDB: 5JUW. http://www.thesgc.org/structures/5JUW/
Proper citation: RRID:Addgene_36196 Copy
Species: Homo sapiens
Genetic Insert: ITCH
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3TUG. http://www.thesgc.org/structures/3TUG/
Proper citation: RRID:Addgene_36197 Copy
Species: Homo sapiens
Genetic Insert: HDAC6.DC
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20133936
Comments: Additional Reference:
Gao etal "Histone Deacetylase 6 Regulates Growth Factor-Induced Actin Remodeling and Endocytosis" MOLECULAR AND CELLULAR BIOLOGY, Dec. 2007, p. 8637–8647
Proper citation: RRID:Addgene_36189 Copy
Species: Homo sapiens
Genetic Insert: H2A
Vector Backbone Description: Vector Backbone:pmTurquoise2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22434194
Comments: Please note that there is a 3bp mismatch between Addgene's quality control sequence and the provided sequence from the depositing lab. This mismatch should not affect function or localization.
Proper citation: RRID:Addgene_36207 Copy
Species: Homo sapiens
Genetic Insert: Golgi targeting sequence
Vector Backbone Description: Vector Backbone:pmTurquoise2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22434194
Proper citation: RRID:Addgene_36205 Copy
Species: Homo sapiens
Genetic Insert: Sec23A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLVX-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21045114
Comments: The cDNA sequence encoding mRuby (Kredel et al., 2009) underwent codon usage
adaptation before gene synthesis (MWG Eurofins) and cloning into pBluescript II
SK(+). mRuby was excised with SalI-EcoRI for insertion into pLVX-Puro (Clontech)
to generate pLVX-Puro–mRuby. A cDNA encoding human Sec23A was amplified
by PCR, gel purified and subcloned via pGEM-T vector (excised with ApaI-XbaI)
and cloned into pLVX-Puro–mRuby.
Proper citation: RRID:Addgene_36158 Copy
Genetic Insert: HE-6
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36279 Copy
Species: Homo sapiens
Genetic Insert: p150Glued
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16772339
Proper citation: RRID:Addgene_36154 Copy
Species: Rattus norvegicus
Genetic Insert: CD200R
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296487
Proper citation: RRID:Addgene_36153 Copy
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