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Species: Arabidopsis thaliana
Genetic Insert: N-Terminal tag Sv40 Nuclear Localization Signal (MASSPPKKKRKVSWKM)
Vector Backbone Description: Backbone Size:2246; Vector Backbone:pICSL01002; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Comments: Level 0 N-Terminal tag module used for GoldenGate assemblies using the MoClo overhang syntax
Proper citation: RRID:Addgene_245548 Copy
Species: Synthetic
Genetic Insert: N-Terminal tag C3_Cysta (metal affinity)
Vector Backbone Description: Backbone Size:2246; Vector Backbone:pICSL01002; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Comments: Level 0 N-Terminal tag module used for GoldenGate assemblies using the MoClo overhang syntax
Proper citation: RRID:Addgene_245537 Copy
Species: Synthetic
Genetic Insert: C-Terminal tag T7 tag + MiniID
Vector Backbone Description: Backbone Size:2244; Vector Backbone:pICSL01003; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Comments: Level 0 C-Terminal tag module used for GoldenGate assemblies using the MoClo overhang syntax
Proper citation: RRID:Addgene_245595 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#12; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241160 Copy
Species: Synthetic
Genetic Insert: BFP
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#15; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241163 Copy
Species: Synthetic
Genetic Insert: BFP
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#14; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241162 Copy
Species: Synthetic
Genetic Insert: creER; acry-mCherry
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#17; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241165 Copy
Species: Synthetic
Genetic Insert: creER
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#19; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241167 Copy
Species: Synthetic
Genetic Insert: creER
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#21; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241169 Copy
Species: Synthetic
Genetic Insert: creER
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#20; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241168 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#4; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Comments: Observed a V214A substitution in EGFP, which should not affect the fluorescence too much.
Proper citation: RRID:Addgene_241152 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#6; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241154 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#8; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241156 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#10; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241158 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pU6a:sgRNA#9; Vector Types:Mini-Golden; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41630603
Proper citation: RRID:Addgene_241157 Copy
Species: Synthetic
Genetic Insert: N-terminal tag with MBP polypeptide
Vector Backbone Description: Backbone Marker:MoClo Toolkit, Addgene Kit #1000000044; Vector Backbone:pAGM9121; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40622926
Proper citation: RRID:Addgene_249631 Copy
Species: Synthetic
Genetic Insert: N-terminal tag with 8x His-residues in tandem with Gb1 polypeptide
Vector Backbone Description: Backbone Marker:MoClo Toolkit, Addgene Kit #1000000044; Vector Backbone:pAGM9121; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40622926
Proper citation: RRID:Addgene_249632 Copy
Species: Synthetic
Genetic Insert: N-terminal tag with 8x His-residues
Vector Backbone Description: Backbone Marker:MoClo Toolkit, Addgene Kit #1000000044; Vector Backbone:pAGM9121; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40622926
Proper citation: RRID:Addgene_249635 Copy
Species: Synthetic
Genetic Insert: N-terminal tag with 6x His-residues
Vector Backbone Description: Backbone Marker:MoClo Toolkit, Addgene Kit #1000000044; Vector Backbone:pAGM9121; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40622926
Proper citation: RRID:Addgene_249634 Copy
Vector Backbone Description: Vector Backbone:pSynORI; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41629321
Comments: J23119-Repressor (LS)-parDE-parCBA-RNA Primer-Target (LS)-Pcym-CymR-Barcode2-SpcR
Proper citation: RRID:Addgene_253203 Copy
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