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Species: Arabidopsis thaliana
Genetic Insert: EGFP reverse complement coding sequence flanked by attB/attP Integrase 4 attachment sites.
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase 4 attachment sites sequences from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991).
Proper citation: RRID:Addgene_127522 Copy
Species: Arabidopsis thaliana
Genetic Insert: EGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase 2 attachment sites sequences from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991).
Proper citation: RRID:Addgene_127521 Copy
Species: Arabidopsis thaliana
Genetic Insert: CaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The CaMV 35S promoter in a reverse complement sequence orientation came from iGEM registry (BBa_K1547006 ) and the attB/P integrases 2, 4 and 5 attatchment sites flanking this sequence came from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014). This cassete was previously synthesized in pBluescript II SK(-) and after cloned using HindIII and BamHI replacing the forward CaMV 35S promoter sequence from a 35S-GFP plasmid construction of Chiu, W. et al. Curr. Biol. 6, 325-330 (1996).
Proper citation: RRID:Addgene_127520 Copy
Species: Arabidopsis thaliana
Genetic Insert: EGFP reverse complement coding sequence flanked by attB/attP Integrase 7 attachment sites.
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase 7 attachment sites sequences from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991).
Proper citation: RRID:Addgene_127524 Copy
Species: Arabidopsis thaliana
Genetic Insert: EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
Vector Backbone Description: Vector Backbone:pBluescript SK(-); Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase Bxb1 attachment sites sequences from Bonnet, J. et al. Science 340, 599-603 (2013) (Genbank KC529327.1); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991).
Proper citation: RRID:Addgene_127528 Copy
Species: Other
Genetic Insert: Integrase 5 coding sequence codon optimized for H. sapiens expression.
Vector Backbone Description: Vector Backbone:pUB-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The integrase 5 coding sequence was synthesized and cloned into pUB-GFP plasmid (Addgene #11155) replacing the EGFP coding sequence. The original integrase coding sequence (before H. sapiens codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127514 Copy
Species: Other
Genetic Insert: Integrase 4 coding sequence codon optimized for H. sapiens expression.
Vector Backbone Description: Vector Backbone:pUB-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The integrase 4 coding sequence was synthesized and cloned into pUB-GFP plasmid (Addgene #11155) replacing the EGFP coding sequence. The original integrase coding sequence (before H. sapiens codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127513 Copy
Species: Other
Genetic Insert: Integrase phiC31 coding sequence codon optimized for H. sapiens expression.
Vector Backbone Description: Vector Backbone:pBluescript II SK(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The UbC promoter and _-globin poly(A) signal sequences are derived from pUB-GFP plasmid (Addgene #11155).
Proper citation: RRID:Addgene_127518 Copy
Species: Other
Genetic Insert: Integrase 13 coding sequence codon optimized for H. sapiens expression.
Vector Backbone Description: Vector Backbone:pUB-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The integrase 13 coding sequence was synthesized and cloned into pUB-GFP plasmid (Addgene #11155) replacing the EGFP coding sequence. The original integrase coding sequence (before H. sapiens codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127517 Copy
Species: Synthetic
Genetic Insert: PtetO7.1_Flagtag_8Glycine linker
Vector Backbone Description: Backbone Size:2894; Vector Backbone:pFA6; Vector Types:Synthetic Biology, PCR based tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34342575
Comments: For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.
Proper citation: RRID:Addgene_127581 Copy
Species: Synthetic
Genetic Insert: PtetO7.1_Flagtag_8Glycine linker
Vector Backbone Description: Backbone Size:3350; Vector Backbone:pFA6; Vector Types:Synthetic Biology, PCR based tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34342575
Comments: For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.
Proper citation: RRID:Addgene_127580 Copy
Species: Synthetic
Genetic Insert: PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
Vector Backbone Description: Backbone Size:3748; Vector Backbone:pRG203MX; Vector Types:Yeast Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34342575
Comments: For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.
Proper citation: RRID:Addgene_127577 Copy
Species: Synthetic
Genetic Insert: PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
Vector Backbone Description: Backbone Size:5108; Vector Backbone:pRG206; Vector Types:Yeast Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34342575
Comments: For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.
Proper citation: RRID:Addgene_127575 Copy
Species: Homo sapiens
Genetic Insert: IRF3
Vector Backbone Description: Vector Backbone:pTRIP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811988
Proper citation: RRID:Addgene_127663 Copy
Species: Homo sapiens
Genetic Insert: cGAS
Vector Backbone Description: Vector Backbone:pTRIP-CMV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811988
Proper citation: RRID:Addgene_127657 Copy
Species: Homo sapiens
Genetic Insert: cGAS
Vector Backbone Description: Vector Backbone:pTRIP-CMV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811988
Proper citation: RRID:Addgene_127656 Copy
Species: Vaccinia Virus
Genetic Insert: VV.Orf76
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12764 Copy
Species: Homo sapiens
Genetic Insert: BLM
Vector Backbone Description: Vector Backbone:pTRIP-CMV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30936263
Proper citation: RRID:Addgene_127641 Copy
Species: Homo sapiens
Genetic Insert: STING
Vector Backbone Description: Vector Backbone:pLentiCRISPRv2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28484079
Proper citation: RRID:Addgene_127640 Copy
Species: Homo sapiens
Genetic Insert: cGAS shRNA
Vector Backbone Description: Vector Backbone:pLKO.1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30936263
Proper citation: RRID:Addgene_127646 Copy
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