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Vector Backbone Description: Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36267 Copy
Species: Mus musculus
Genetic Insert: Dynamin 3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17463283
Proper citation: RRID:Addgene_36265 Copy
Species: Mus musculus
Genetic Insert: Dynamin 2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17463283
Comments: HA-tagged mouse dynamin 2 flanked by KpnI and XbaI sites. BamHI between N-terminal HA tag and beginning of dynamin coding seq.
Proper citation: RRID:Addgene_36264 Copy
Species: Homo sapiens
Genetic Insert: MBD3
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36371 Copy
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-1 (NOT pEGFP-N1); Vector Types:Fluorescent protein cassette for sub-cloning; Bacterial Resistance:Kanamycin
Comments: Plasmid carrying monomeric (A206K) EGFP for C-terminal tagging
Proper citation: RRID:Addgene_36409 Copy
Species: Homo sapiens
Genetic Insert: YY1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36358 Copy
Species: Homo sapiens
Genetic Insert: Suv39H2
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36344 Copy
Species: Homo sapiens
Genetic Insert: Dot1L
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36339 Copy
Species: Homo sapiens
Genetic Insert: Ehmt1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36338 Copy
Vector Backbone Description: Backbone Size:5678; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20671728
Proper citation: RRID:Addgene_36295 Copy
Genetic Insert: M13-DDRFP-B1
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22444590
Proper citation: RRID:Addgene_36293 Copy
Genetic Insert: ddRFPA1-CaM
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22444590
Proper citation: RRID:Addgene_36292 Copy
Species: Homo sapiens
Genetic Insert: shRNA of beta-catenin-1
Vector Backbone Description: Backbone Marker:Biosettia; Backbone Size:10275; Vector Backbone:pLV-H1TetO-RFP-Puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22645348
Proper citation: RRID:Addgene_36297 Copy
Species: Synthetic
Genetic Insert: 4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16720273
Comments: Genetically encoded, ratiometric, calcium biosensor that contains enhanced CFP and circularly permuted Venus 173. D3cpv does not bind wild-type calmodulin. It has a Kd' of 0.6 uM.
See also Palmer & Tsien (2006) Nature Protocols.
The small discrepancy between the Addgene and depositor's sequence should not alter the function of the plasmid.
Proper citation: RRID:Addgene_36324 Copy
Vector Backbone Description: Backbone Marker:Vieira and Messing, 1991; Backbone Size:3868; Vector Backbone:pUK21; Vector Types:Bacterial Expression, Suicide vector for R. rubrum; Bacterial Resistance:Kanamycin
Comments: Constructed by D. P. Lies and G. P. Roberts
Proper citation: RRID:Addgene_36328 Copy
Genetic Insert: shRNA of scramble
Vector Backbone Description: Backbone Marker:Biosettia; Backbone Size:10275; Vector Backbone:pLV-H1TetO-RFP-Puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22645348
Comments: The plasmid backbone is TetO-H1-shRNA-EF1a-TetR-IRES-RFP-Puro. It is a all-in-one inducible shRNA knockdown lentiviral plasmid.
The paper associated with this plasmid is "Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling"
Proper citation: RRID:Addgene_36311 Copy
Species: Mus musculus
Genetic Insert: mTERT
Vector Backbone Description: Backbone Marker:Bob Weinberg (Addgene Plasmid #1764); Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21732358
Proper citation: RRID:Addgene_36413 Copy
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Plasmid carrying monomeric (A206K) EGFP for N-terminal tagging
Proper citation: RRID:Addgene_36412 Copy
Species: Homo sapiens
Genetic Insert: breakpoint cluster region
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17116687
Comments: Made by mutagenesis directly on plasmid Bcr-ECFP using a Stratagene Quick Change kit. In Dbl, mutation of residues N673 and D674 completely abolished its activity as a GEF towards Rac. These residues are highly conserved among DH domains and are also conserved as residues N689/ E690 in Bcr. We therefore mutated these residues in Bcr and introduced the mutants into the RAW 264.7 macrophage cell line. However, the construct did not caused morphological changes in these cells. Constructed by Young-Jin Cho.
Proper citation: RRID:Addgene_36418 Copy
Species: Homo sapiens
Genetic Insert: EHD1 EH domain
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_36459 Copy
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