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Species: Homo sapiens
Genetic Insert: BTLAecto&tm-PD-1int
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32437509
Proper citation: RRID:Addgene_180795 Copy
Species: Homo sapiens
Genetic Insert: Dystrophin
Vector Backbone Description: Backbone Marker:Phramacia; Backbone Size:4969; Vector Backbone:pGEX-2TK (Pharmacia); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10355629
Proper citation: RRID:Addgene_18070 Copy
Species: Homo sapiens
Genetic Insert: Dystrophin
Vector Backbone Description: Backbone Marker:Phramacia; Backbone Size:4969; Vector Backbone:pGEX-2TK (Pharmacia); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10355629
Proper citation: RRID:Addgene_18073 Copy
Species: Homo sapiens
Genetic Insert: Dystrophin
Vector Backbone Description: Backbone Marker:Phramacia; Backbone Size:4969; Vector Backbone:pGEX-2TK (Pharmacia); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10355629
Proper citation: RRID:Addgene_18075 Copy
Species: Homo sapiens
Genetic Insert: Human Epididymis Protein 4
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_18100 Copy
Species: Homo sapiens
Genetic Insert: Human Epididymis Protein 4
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_18101 Copy
Species: Homo sapiens
Genetic Insert: Dystrophin
Vector Backbone Description: Backbone Marker:Phramacia; Backbone Size:4969; Vector Backbone:pGEX-2TK (Pharmacia); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10355629
Proper citation: RRID:Addgene_18069 Copy
Species: Homo sapiens
Genetic Insert: Secretory Leucocyte Protease Inhibitor
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_18103 Copy
Species: Homo sapiens
Genetic Insert: BTLAint (AA190-289, Y243F, Y257F, Y282F)-TwinStrep
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32437509
Proper citation: RRID:Addgene_180806 Copy
Species: Homo sapiens
Genetic Insert: Dystrophin
Vector Backbone Description: Backbone Marker:Phramacia; Backbone Size:4969; Vector Backbone:pGEX-2TK (Pharmacia); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10355629
Proper citation: RRID:Addgene_18081 Copy
Species: Homo sapiens
Genetic Insert: Dystrophin
Vector Backbone Description: Backbone Marker:Phramacia; Backbone Size:4969; Vector Backbone:pGEX-2TK (Pharmacia); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10355629
Proper citation: RRID:Addgene_18082 Copy
Species: Homo sapiens
Genetic Insert: Dystrophin
Vector Backbone Description: Backbone Marker:Phramacia; Backbone Size:4969; Vector Backbone:pGEX-2TK (Pharmacia); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10355629
Proper citation: RRID:Addgene_18077 Copy
Species: Homo sapiens
Genetic Insert: Dystrophin
Vector Backbone Description: Backbone Marker:Phramacia; Backbone Size:4969; Vector Backbone:pGEX-2TK (Pharmacia); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10355629
Proper citation: RRID:Addgene_18079 Copy
Species: Homo sapiens
Genetic Insert: h-RasV12G
Vector Backbone Description: Backbone Marker:Constructed from multiple sources; Backbone Size:6664; Vector Backbone:N/A; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18378907
Proper citation: RRID:Addgene_18113 Copy
Species: Homo sapiens
Genetic Insert: Raptor
Vector Backbone Description: Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18439900
Comments: Addgene #1859 was PCR amplified with primers containing EcoRI and XhoI, then ligated into pBABE-hygro and fully sequenced
Proper citation: RRID:Addgene_18116 Copy
Species: Homo sapiens
Genetic Insert: Raptor
Vector Backbone Description: Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18439900
Comments: Addgene #1859 was mutated at two serine residues, then PCR amplified with primers containing EcoRI and XhoI, then ligated into pBABE-hygro and fully sequenced
Proper citation: RRID:Addgene_18117 Copy
Species: Homo sapiens
Genetic Insert: PD-1 (BTLA ITIM)
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34734802
Proper citation: RRID:Addgene_180817 Copy
Species: Homo sapiens
Genetic Insert: BTLA (PD-1 ITIM)
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34734802
Proper citation: RRID:Addgene_180818 Copy
Species: Homo sapiens
Genetic Insert: c-MYC
Vector Backbone Description: Backbone Marker:Dr. Robert Hawley of George Washington University; Backbone Size:5660; Vector Backbone:MSCV IRES GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Comments: The MSCV vector is described in; Hawley, R.G., Lieu, F.H.L., Fong, A.Z.C., and Hawley, T.S. Versatile retroviral vectors for potential use in gene therapy. Gene Ther. 1: 136-138, 1994.
Use of the construct is described in; Park, I., Zhao, R., West, J.A., Yabuuchi, A., Huo, H., Ince, T.A., Lerou, P.H., Lensch, M.W., and Daley, G.Q. Reprogramming of human somatic cells to pluripotency with defined factors. Nature, 451, 141-146, 2008.
Please note that the assembled sequence presented here is not 100% reflective of the actual sequence. See the Addgene diagnostic digest (found under the Resource Information section of the plasmid page) for additional information on the expected plasmid size. Addgene has found another HindIII site at the end of the MYC gene (see our quality control sequence).
Proper citation: RRID:Addgene_18119 Copy
Species: Homo sapiens
Genetic Insert: PD-1
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34734802
Proper citation: RRID:Addgene_180819 Copy
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