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Species: Synthetic
Genetic Insert: sgRNArodA
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149569 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNAftsI
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S(RBSmut); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149590 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S(RBSmut); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Comments: Addgene QC identified an IS element present in the backbone sequence. The depositing lab does not believe this impacts the functional use of the plasmid but requesting scientists should validate expected behavior prior to experimentation.
Proper citation: RRID:Addgene_149588 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S(-10TC); Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149589 Copy
Species: Synthetic
Genetic Insert: sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149627 Copy
Species: Synthetic
Genetic Insert: sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149628 Copy
Species: Synthetic
Genetic Insert: sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2G_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149629 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNAmreB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149654 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNArodA
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149655 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNArodA
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149658 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI, sgRNArodA
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9S(-10AC2); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149660 Copy
Species: Synthetic
Genetic Insert: 14X miR-146a binding sites
Vector Backbone Description: Backbone Size:8518; Vector Backbone:pSCALPS_mCherry_GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32205878
Proper citation: RRID:Addgene_149718 Copy
Species: Synthetic
Genetic Insert: TQM IL13 CAR
Vector Backbone Description: Vector Backbone:pXL001; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32405577
Proper citation: RRID:Addgene_154054 Copy
Species: Synthetic
Genetic Insert: CRE_U5intron
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet/ TSL SynBio; Backbone Size:2221; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33072173
Proper citation: RRID:Addgene_154069 Copy
Species: Synthetic
Genetic Insert: engineered bacterial rRNAs operon under control of pL promotor
Vector Backbone Description: Backbone Size:3414; Vector Backbone:Synthetic; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32313034
Proper citation: RRID:Addgene_154125 Copy
Species: Synthetic
Genetic Insert: orthogonal gfp
Vector Backbone Description: Backbone Size:4354; Vector Backbone:Synthetic; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32313034
Proper citation: RRID:Addgene_154127 Copy
Species: Synthetic
Genetic Insert: orthogonal firefly luciferase
Vector Backbone Description: Backbone Size:4354; Vector Backbone:Synthetic; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32313034
Comments: Addgene QC NGS analysis identified multiple sequence discrepancies, relative to the sequence provided by the depositing laboratory. The depositing laboratory has indicated that these are not a concern for plasmid function.
Proper citation: RRID:Addgene_154128 Copy
Species: Synthetic
Genetic Insert: his6-TEV-protein A-KanR
Vector Backbone Description: Vector Backbone:pBS1479; Vector Types:PCR-based tagging in yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26670050
Proper citation: RRID:Addgene_154085 Copy
Species: Synthetic
Genetic Insert: [5HA]-P2A-tdTomato-[3HA]
Vector Backbone Description: Backbone Marker:Derived from pX458 (Zhang lab); Backbone Size:9500; Vector Backbone:pQCi; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33616439
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.25.008276v4 for bioRxiv preprint.
Proper citation: RRID:Addgene_154095 Copy
Species: Synthetic
Genetic Insert: P2A-tagBFP
Vector Backbone Description: Backbone Marker:Derived from pX458 (Zhang lab); Backbone Size:9500; Vector Backbone:pQCi; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33616439
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.25.008276v4 for bioRxiv preprint.
Proper citation: RRID:Addgene_154096 Copy
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