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Vector Backbone Description: Vector Backbone:pLentiCRISPRv2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30936263
Proper citation: RRID:Addgene_127644 Copy
Species: Homo sapiens
Genetic Insert: Tom70
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31894645
Proper citation: RRID:Addgene_127633 Copy
Species: Mus musculus
Genetic Insert: Trak1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24671417
Proper citation: RRID:Addgene_127621 Copy
Species: Other
Genetic Insert: Sindbis Glycoproteins
Vector Backbone Description: Vector Backbone:pSin; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31280962
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_127694 Copy
Species: Other
Genetic Insert: Sindbis Capsid
Vector Backbone Description: Vector Backbone:pSin; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31280962
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_127693 Copy
Species: Other
Genetic Insert: Sindbis Structural Genome
Vector Backbone Description: Vector Backbone:pSRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31280962
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_127692 Copy
Species: Homo sapiens
Genetic Insert: shNS
Vector Backbone Description: Backbone Size:13320; Vector Backbone:pTRIPZ RFP+; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28598415
Proper citation: RRID:Addgene_127696 Copy
Species: Mus musculus
Genetic Insert: IkB alpha M
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8864120
Comments: IkB alpha M was generated by digestion of plasmids pCMX-IkBS32/36A and pCMX-IkBMutF with Eco NI and Bst EII and ligation of the small S32/36A fragment to the large vector/IkBMutF fragment.
Proper citation: RRID:Addgene_12329 Copy
Species: Mus musculus
Genetic Insert: IkB alpha
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8864120
Proper citation: RRID:Addgene_12331 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated proteins 1A/1B light chain 3B
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pDEST-CMV-N-Tandem-mCherry-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123235 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pLenti-C-MYC-DDK Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30373810
Proper citation: RRID:Addgene_123299 Copy
Species: Homo sapiens
Genetic Insert: CSNK1A1
Vector Backbone Description: Vector Backbone:pLCE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29954751
Proper citation: RRID:Addgene_123320 Copy
Species: Homo sapiens
Genetic Insert: ArchT-BFP2
Vector Backbone Description: Backbone Marker:Michael Lin lab; Backbone Size:6105; Vector Backbone:pcDNA3.1/puro-CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30638447
Proper citation: RRID:Addgene_123312 Copy
Species: Homo sapiens
Genetic Insert: GPCR activation based NE sensor GRAB_NE1m
Vector Backbone Description: Backbone Size:4495; Vector Backbone:pAAV vector; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30922875
Comments: Please visit https://www.biorxiv.org/content/early/2018/10/23/449546.full.pdf for BioRxiv preprint.
Proper citation: RRID:Addgene_123308 Copy
Species: Synthetic
Genetic Insert: EGFP-NLS
Vector Backbone Description: Backbone Size:9004; Vector Backbone:pRG01; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30962622
Proper citation: RRID:Addgene_123363 Copy
Species: Drosophila melanogaster
Genetic Insert: U6:3-gRNA
Vector Backbone Description: Backbone Marker:Fillip Port; Vector Backbone:pCFD3-dU6-3gRNA (Addgene 49410); Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: When describing the use of this plasmid in publications, please state that it is derived from pCFD3 and cite:
Port et al. Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):E2967-76. doi: 10.1073/pnas.1405500111
In pCFD3.1, the BbsI sites have been mutated in mini-white, which allows use of the remaining BbsI sites for gRNA cloning (using the protocol for pCFD3 (https://www.crisprflydesign.org/plasmids/)).
Proper citation: RRID:Addgene_123366 Copy
Species: Mus musculus
Genetic Insert: GSDMD
Vector Backbone Description: Backbone Marker:Berkeley Structural Genomics Center; Backbone Size:6494; Vector Backbone:pDB-His-MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30361383
Proper citation: RRID:Addgene_123365 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:8500; Vector Backbone:pBOB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14742905
Proper citation: RRID:Addgene_12337 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CKAR2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29968564
Proper citation: RRID:Addgene_123348 Copy
Species: Homo sapiens
Genetic Insert: NEP-X
Vector Backbone Description: Backbone Size:8500; Vector Backbone:pBOB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14742905
Comments: The sequence and map shown are for the wild-type Nep plasmid. The NepX plasmid contains an inactivating E585V mutation in the Nep gene.
Proper citation: RRID:Addgene_12340 Copy
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