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Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725303.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149188 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF3a protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724391.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149221 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp2
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725298.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149222 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 E (envelope)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724392.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149224 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF8 protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724396.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149225 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp10
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725306.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149226 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Flp recombinase
Vector Backbone Description: Backbone Size:5383; Vector Backbone:pAAV-nef-CIAO; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31843925
Comments: Full name: pAAV-nef-AO-lox66-STOP-lox71-Flp-hGH-pA
Proper citation: RRID:Addgene_149294 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Flp recombinase
Vector Backbone Description: Backbone Size:5383; Vector Backbone:pAAV-nef-CIAO2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31843925
Comments: The CIAO2 construct replaces the lox66/71 pair used in CIAO with the lox43/44 pair to further decrease leak expression - not used in the original publication. Full name: pAAV-nef-AO-lox43-STOP-lox44-Flp-hGH-pA
Proper citation: RRID:Addgene_149296 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153103 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153102 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Acid Phosphatase SP
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33980981
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.07.22.216432v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153494 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Killer Protein SP
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33980981
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.07.22.216432v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153498 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HO endonuclease
Vector Backbone Description: Backbone Marker:Addgene plasmid # 64521; Backbone Size:3833; Vector Backbone:pRG203MX; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33053188
Comments: Linearize plasmid with AscI for integration into the yeast genome. See Gnuegge et al., 2016 (https://pubmed.ncbi.nlm.nih.gov/26647923/) for details.
Proper citation: RRID:Addgene_154819 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HO endonuclease
Vector Backbone Description: Backbone Marker:Addgene plasmid # 64535; Backbone Size:5193; Vector Backbone:pRG205MX; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33053188
Comments: Linearize plasmid with AscI for integration into the yeast genome. See Gnuegge et al., 2016 (https://pubmed.ncbi.nlm.nih.gov/26647923/) for details.
Proper citation: RRID:Addgene_154815 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Orp1
Vector Backbone Description: Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_155043 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: QFGal4-switch
Vector Backbone Description: Backbone Size:7424; Vector Backbone:pT2-Ip2GI; Vector Types:Cre/Lox, Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32697972
Comments: Vector backbone derived from ubi:zebrabow (Plasmid #118219). mScarletNLS was codon-optimized for zebrafish by Tod Thiele (UTSC). ubi:QFGal4-switch can be used for Cre-dependent QFGal4 expression in lineage tracing experiments
Proper citation: RRID:Addgene_155125 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35 NM
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pAED4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17495929
Comments: Protein expression inducible by IPTG. Sc/Ca = Saccharomyces cerevisiae / Candida albicans chimera (residues ScNM 1-40, CaNM 49-141, ScNM 124-254), NM = N-terminal and Middle domains of Sup35.
Lindquist plasmid # 8635.
Proper citation: RRID:Addgene_15602 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35 NM
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pAED4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17495929
Comments: Protein expression inducible by IPTG. Sc/Ca = Saccharomyces cerevisiae / Candida albicans chimera (residues ScNM 1-40, CaNM 49-141, ScNM 124-254), NM = N-terminal and Middle domains of Sup35.
Lindquist plasmid # 8634.
Proper citation: RRID:Addgene_15601 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SNF5
Vector Backbone Description: Backbone Size:0; Vector Backbone:p316 CUP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832049
Comments: Figure 5 in article.
Lindquist lab number: 7151
Proper citation: RRID:Addgene_15597 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PUF2
Vector Backbone Description: Backbone Size:0; Vector Backbone:p316 CUP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832049
Comments: Figure 5 in article.
Lindquist lab number: 8030
Proper citation: RRID:Addgene_15595 Copy
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