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Species: Homo sapiens
Genetic Insert: TRBV4-1
Vector Backbone Description: Vector Backbone:pUC57-kanamycin; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30150207
Comments: Cloning Method: Golden Gate.
Proper citation: RRID:Addgene_123411 Copy
Species: Homo sapiens
Genetic Insert: TRBV25-1
Vector Backbone Description: Vector Backbone:pUC57-kanamycin; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30150207
Comments: Cloning Method: Golden Gate.
Proper citation: RRID:Addgene_123456 Copy
Vector Backbone Description: Backbone Marker:Verma Lab; Backbone Size:7807; Vector Backbone:pCLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8764092
Comments: A 3.1-kb fragment of LXSN, spanning from the SacI site in the 5'LTR near the U3-R junction to the AccI site just beyond the 3'LTR, was cloned into the 4.5-kb CMV expression construct, CMX-low, at a corresponding SacI site within the CMV immediate early enhancer-promoter.
NOTE: The depositor map indicated an EcoRI site present in this plasmid, but that site is ablated by a small insert.
Note that the full sequence and subsequent map are assembled and have not been verified by full sequencing. Please take this into account when submitting this plasmid to diagnostic digests.
Proper citation: RRID:Addgene_12343 Copy
Species: Mus musculus
Genetic Insert: VCP R313K
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Comments: *Please note: Addgene's quality control sequencing results identified an I206V amino acid residue substitution in the VCP sequence when compared to GenBank reference NP_033529.3.
Proper citation: RRID:Addgene_12345 Copy
Species: Mus musculus
Genetic Insert: dishevelled segment polarity protein 2
Vector Backbone Description: Backbone Size:5079; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31073040
Proper citation: RRID:Addgene_123587 Copy
Species: Mus musculus
Genetic Insert: frizzled class receptor 6
Vector Backbone Description: Backbone Size:4692; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31073040
Proper citation: RRID:Addgene_123589 Copy
Species: Synthetic
Genetic Insert: NLS-nCas9-NLS-P2A-EGFP
Vector Backbone Description: Backbone Marker:pCMV-BE1 (Addgene #73019); Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30995674
Proper citation: RRID:Addgene_123616 Copy
Species: Synthetic
Genetic Insert: nCas9-UGI-NLS-P2A-EGFP
Vector Backbone Description: Backbone Marker:pSQT817 (Addgene #53373); Backbone Size:4843; Vector Backbone:CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30995674
Proper citation: RRID:Addgene_123611 Copy
Species: Mus musculus
Genetic Insert: Connexin 36-GCaMP3 fusion
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3800; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32179580
Proper citation: RRID:Addgene_123604 Copy
Species: Mus musculus
Genetic Insert: valosin containing protein
Vector Backbone Description: Backbone Size:5034; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31073040
Proper citation: RRID:Addgene_123600 Copy
Species: Other
Genetic Insert: FR13_APikL2
Vector Backbone Description: Backbone Size:2626; Vector Backbone:pUC57-Kan; Vector Types:Golden Gate; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32460610
Proper citation: RRID:Addgene_123648 Copy
Species: Other
Genetic Insert: PCV2
Vector Backbone Description: Vector Backbone:pTD68; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30271937
Proper citation: RRID:Addgene_123643 Copy
Species: Synthetic
Genetic Insert: BsCas12a
Vector Backbone Description: Vector Backbone:pCAG-2AeGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30717767
Proper citation: RRID:Addgene_123633 Copy
Species: Synthetic
Genetic Insert: LpCas12a
Vector Backbone Description: Vector Backbone:pCAG-2AeGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30717767
Proper citation: RRID:Addgene_123635 Copy
Species: Herpes virus
Genetic Insert: thymidine kinase
Vector Backbone Description: Backbone Size:3938; Vector Backbone:pFA6a-kanMX6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15157888
Proper citation: RRID:Addgene_12382 Copy
Species: Synthetic
Genetic Insert: VP16::PYLcs-T2A-GAL4DBD::ABIcs
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 3 parts are generally reserved for coding sequences in the MTK. Part sequence is from 10.1126/scisignal.2001449 Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123742 Copy
Species: Other
Genetic Insert: sgRNA1 -hCLYBL
Vector Backbone Description: Vector Backbone:MTK234_002; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 234 parts are coupled promoter-coding sequence-terminator such as guide RNA cassettes, GFP dropouts in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123910 Copy
Species: Synthetic
Genetic Insert: CMV-Hygro-bgPA
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 6 parts can be components of the destination backbone such as resistance cassette, nuclear marker, integrase recognition sites in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123863 Copy
Species: Other
Genetic Insert: rglb pA
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 4b parts are polyadenylation signals used when a type 4a has been included as a coding sequence. Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123842 Copy
Species: Synthetic
Genetic Insert: hCLYBL CAS9 Destination - HygroR
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31686495
Comments: Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123977 Copy
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