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Species: Synthetic
Genetic Insert: Promoter 1D4 (orthogonal T7-lac variant)
Vector Backbone Description: Backbone Marker:Koffas Lab, Addgene Plasmid #49795; Backbone Size:5147; Vector Backbone:pETM6; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27079979
Comments: Constructed using degenerate site directed mutagenesis of T7-lac promoter region. Also encodes mCherry reporter.
Proper citation: RRID:Addgene_73423 Copy
Species: Synthetic
Genetic Insert: Promoter 5F5 (orthogonal T7-lac variant)
Vector Backbone Description: Backbone Marker:Koffas Lab, Addgene Plasmid #49795; Backbone Size:5147; Vector Backbone:pETM6; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27079979
Comments: Constructed using degenerate site directed mutagenesis of T7-lac promoter region. Also encodes mCherry reporter.
Proper citation: RRID:Addgene_73422 Copy
Species: Synthetic
Genetic Insert: Repressor C4 (orthogonal T7-lac repressor)
Vector Backbone Description: Backbone Marker:Luciano Marraffini (Addgene plasmid # 46569); Backbone Size:9296; Vector Backbone:pdCas9; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27079979
Comments: Also encodes dCas9 and tracrRNA.
Proper citation: RRID:Addgene_73427 Copy
Species: Synthetic
Genetic Insert: Repressor 1E4 (orthogonal T7-lac repressor)
Vector Backbone Description: Backbone Marker:Luciano Marraffini (Addgene plasmid # 46569); Backbone Size:9296; Vector Backbone:pdCas9; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27079979
Comments: Also encodes dCas9 and tracrRNA.
Proper citation: RRID:Addgene_73436 Copy
Species: Synthetic
Genetic Insert: Repressor 3A2 (orthogonal T7-lac repressor)
Vector Backbone Description: Backbone Marker:Luciano Marraffini (Addgene plasmid # 46569); Backbone Size:9296; Vector Backbone:pdCas9; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27079979
Comments: Also encodes dCas9 and tracrRNA.
Proper citation: RRID:Addgene_73435 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:3356; Vector Backbone:pAAVS1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26971820
Proper citation: RRID:Addgene_73500 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Backbone Size:3356; Vector Backbone:pAAVS1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26971820
Proper citation: RRID:Addgene_73503 Copy
Species: Synthetic
Genetic Insert: attR1/attR2 cassette
Vector Backbone Description: Vector Backbone:pTol2005b; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73484 Copy
Species: Synthetic
Genetic Insert: lmo2 promoter:mCherry-attR1-attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73489 Copy
Species: Synthetic
Genetic Insert: lmo2 promoter:EGFP-attR1-attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73488 Copy
Species: Synthetic
Genetic Insert: pAzFRS.2.t1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26571098
Comments: Note: This plasmid exists as a tandem dimer approximately 12 kb in length. Please see the diagnostic digest in the Resource Information section.
Proper citation: RRID:Addgene_73546 Copy
Species: Synthetic
Genetic Insert: pAcFRS.1.t1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26571098
Proper citation: RRID:Addgene_73545 Copy
Species: Synthetic
Genetic Insert: pAcFRS.2.t1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26571098
Proper citation: RRID:Addgene_73544 Copy
Species: Synthetic
Genetic Insert: pAzFRS.1.t1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26571098
Proper citation: RRID:Addgene_73547 Copy
Species: Synthetic
Genetic Insert: fli1ep:6xMyc-attR1-attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73490 Copy
Species: Synthetic
Genetic Insert: fli1ep:mCherry-attR1-attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73493 Copy
Species: Synthetic
Genetic Insert: Clover_PUFc
Vector Backbone Description: Vector Backbone:pAC90-pmax-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73689 Copy
Species: Synthetic
Genetic Insert: Clover_PUFa
Vector Backbone Description: Vector Backbone:pAC90-pmax-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73688 Copy
Species: Synthetic
Genetic Insert: BFPKRAB_PUFa
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73692 Copy
Species: Synthetic
Genetic Insert: mRuby2_PUFa
Vector Backbone Description: Vector Backbone:pAC90-pmax-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73690 Copy
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