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Species: Mus musculus
Genetic Insert: tdTomato-T2A-mSyp::EGFP
Vector Backbone Description: Backbone Marker:Connie Cepko (Addgene plasmid # 13769); Backbone Size:6107; Vector Backbone:pCALNL; Vector Types:Mammalian Expression, pCAGEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36386881
Comments: Suggested primers to sequence the insert:
5' ccttcttgacgagttcttctg 3'
5' acgtctccgcatgtcagaag 3'
5' aaggcctgtccgatgtgaag 3'
5' ttcaggaagccaaacaccac 3'
5' GCTTGCCGTAGGTGGCATC 3'
5' CAGGAAACAGCTATGAC 3'
Proper citation: RRID:Addgene_191213 Copy
Species: Mus musculus
Genetic Insert: 3'UTR mMov10
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3560; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35856394
Comments: Please visit https://doi.org/10.1101/2021.11.09.467897 for bioRxiv preprint.
Proper citation: RRID:Addgene_178910 Copy
Species: Mus musculus
Genetic Insert: Cdk2ap1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression, Nonviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34644528
Proper citation: RRID:Addgene_178033 Copy
Species: Mus musculus
Genetic Insert: mTurquoise2
Vector Backbone Description: Backbone Marker:Knouse Lab; Vector Backbone:pLentiCRISPRv2-Stuffer-HepmTurquoise2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36643909
Proper citation: RRID:Addgene_192828 Copy
Species: Mus musculus
Genetic Insert: mD6Ertd527e
Vector Backbone Description: Backbone Size:2886; Vector Backbone:pSV40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36482406
Proper citation: RRID:Addgene_192223 Copy
Species: Mus musculus
Genetic Insert: Cdk2ap1
Vector Backbone Description: Backbone Marker:David Bartel; Backbone Size:7657; Vector Backbone:pMSCV PIG (Puro IRES GFP empty vector); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34644528
Proper citation: RRID:Addgene_178031 Copy
Species: Mus musculus
Genetic Insert: Mouse Liver CRISPR Knockout Library
Vector Backbone Description: Vector Backbone:pLentiCRISPRv2-Stuffer-HepmCherry; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36643909
Proper citation: RRID:Addgene_192824 Copy
Species: Mus musculus
Genetic Insert: Vamp2
Vector Backbone Description: Backbone Marker:Zuchero lab; Backbone Size:4561; Vector Backbone:pBZ-167 AAV CMV promoter base vector; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36151203
Comments: Uribina...& Gupton; PMID: 29351997
Gow...& Lazzarini; PMID:1383235
Pan... & Musacchio; PMID: 28059702.
Please visit https://www.biorxiv.org/content/10.1101/2022.07.08.498895v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190153 Copy
Species: Mus musculus
Genetic Insert: Vamp2
Vector Backbone Description: Backbone Marker:Zuchero lab; Vector Backbone:pBZ-167 AAV CMV promoter base vector; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36151203
Comments: Vamp2-pHluorin was cloned from a construct kindly provided by Prof. Stephanie Gupton, from this paper: PMID: 29351997.
Please visit https://www.biorxiv.org/content/10.1101/2022.07.08.498895v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190151 Copy
Species: Mus musculus
Genetic Insert: SspBmicro-mCherry-p60
Vector Backbone Description: Backbone Marker:Lukas Kapitein lab; Backbone Size:6000; Vector Backbone:pB80; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36174574
Proper citation: RRID:Addgene_190168 Copy
Species: Mus musculus
Genetic Insert: PTK2
Vector Backbone Description: Backbone Size:4856; Vector Backbone:pFastBac Htb; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35049497
Proper citation: RRID:Addgene_186141 Copy
Species: Mus musculus
Genetic Insert: PTK2
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35049497
Proper citation: RRID:Addgene_186149 Copy
Species: Mus musculus
Genetic Insert: PTK2
Vector Backbone Description: Backbone Size:4856; Vector Backbone:pFastBac Htb; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35049497
Proper citation: RRID:Addgene_186144 Copy
Species: Mus musculus
Genetic Insert: gRNA targeting Arid1b
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Lentiviral, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33608386
Comments: Please note there is a R24S mutation in Cre. Depositor confirms this does not affect plasmid function.
Proper citation: RRID:Addgene_173574 Copy
Species: Mus musculus
Genetic Insert: Msh2_gRNA
Vector Backbone Description: Backbone Size:12992; Vector Backbone:LentiCRISPR V2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31303577
Proper citation: RRID:Addgene_186156 Copy
Species: Mus musculus
Genetic Insert: CARD domain of mouse caspase-1 fused to catalytic domain of mouse caspase-11
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pMSCV-IRES-EGFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34734155
Proper citation: RRID:Addgene_183391 Copy
Species: Mus musculus
Genetic Insert: cGAS
Vector Backbone Description: Backbone Marker:Adam Karpf; Backbone Size:8185; Vector Backbone:pCW57-MCS1-P2A-MCS2 (Hygro); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35538147
Proper citation: RRID:Addgene_185459 Copy
Species: Mus musculus
Genetic Insert: GTP-binding nuclear protein Ran
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34424312
Comments: For protein expression, transform into BL21(DE3) E. coli because the pET vector system requires T7 RNA polymerase for expression. Induce with 1 mM ITPG for 2h at 28 °C.
Proper citation: RRID:Addgene_186271 Copy
Species: Mus musculus
Genetic Insert: GTP-binding nuclear protein Ran
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34424312
Comments: For protein expression, transform into BL21(DE3) E. coli because the pET vector system requires T7 RNA polymerase for expression. Induce with 1 mM ITPG for 2h at 28 °C.
Proper citation: RRID:Addgene_186270 Copy
Species: Mus musculus
Genetic Insert: Caspase-11 (AA100-373)
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34734155
Proper citation: RRID:Addgene_183382 Copy
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