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Species: Gallus gallus
Genetic Insert: chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
Vector Backbone Description: Backbone Size:3055; Vector Backbone:pNG3; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29386245
Comments: Kudo, T., and S. Sutou. 2005. Usage of putative chicken U6 promoters for vector-based RNA interference. J Reprod Dev. 51:411-417.
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2014 Dec 10. doi: 10.1038/nature14136. 10.1038/nature14136 PubMed 25494202
Please visit https://www.biorxiv.org/content/early/2017/05/08/135525 for bioRxiv preprint.
Proper citation: RRID:Addgene_92394 Copy
Species: Gallus gallus
Genetic Insert: dorsalin-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9314535
Comments: Plasmid History:
To place dsl-1myc after the twhh promoter, the DNA insert of dsl-1myc was first subcloned into the EcoRI site of expression vector pCS2+, which has a cytomegalovirus promoter and a polyadenylation site. The resulting plasmids were named pCS2+-dsl-1myc. To link dsl-1myc to the twhh promoter, the dsl-1myc insert was released from pCS2+-dsl-1myc by BamHI and XhoI digestion and then subcloned into pCS-twhh-beta -gal-vec BamHI and XhoI sites by replacing the beta -gal sequence. The final construct, pCS-twhh-dsl-1myc, contains the 5.2-kb twhh promoter and dsl-1myc.
Notes for Use:
Target dorsalin-1 expression in zebrafish notochord.
Citation: Du et al., 1997, J. Cell Biology.
Proper citation: RRID:Addgene_17156 Copy
Species: Gallus gallus
Genetic Insert: Src
Vector Backbone Description: Backbone Marker:GE-Healthcare; Backbone Size:12803; Vector Backbone:TRIPZ-non-silencing shRNA control; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26216901
Proper citation: RRID:Addgene_83469 Copy
Species: Gallus gallus
Genetic Insert: Gata1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2867; Vector Backbone:pGEM3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2249770
Proper citation: RRID:Addgene_83829 Copy
Species: Gallus gallus
Genetic Insert: CXADR
Vector Backbone Description: Backbone Marker:Sigma; Vector Backbone:pFlag-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_87238 Copy
Species: Gallus gallus
Genetic Insert: CXADR
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:pGEX-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20181587
Proper citation: RRID:Addgene_87233 Copy
Species: Gallus gallus
Genetic Insert: CXADR
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20181587
Proper citation: RRID:Addgene_87231 Copy
Species: Gallus gallus
Genetic Insert: CSPG5
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:pAS2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_89407 Copy
Species: Gallus gallus
Genetic Insert: CSPG5
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pGBT9; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_89403 Copy
Species: Gallus gallus
Genetic Insert: NFASC
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pGBT9; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11152476
Comments: Note: This plasmid contains amino acids 1160 - 1272 of the NFASC insert (NP_001004493.1).
Proper citation: RRID:Addgene_89418 Copy
Species: Gallus gallus
Genetic Insert: CNTN2
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_89413 Copy
Species: Gallus gallus
Genetic Insert: RSK
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pKH3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12832467
Comments: See author's map for Addgene plasmid 8998 (pKH3-avian RSK1).
Proper citation: RRID:Addgene_8981 Copy
Species: Gallus gallus
Genetic Insert: RSK
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pKH3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11585927
Proper citation: RRID:Addgene_8998 Copy
Species: Gallus gallus
Genetic Insert: Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1)
Vector Backbone Description: Backbone Size:6820; Vector Backbone:pMT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28402863
Proper citation: RRID:Addgene_79881 Copy
Species: Gallus gallus
Genetic Insert: vinculin
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_80025 Copy
Species: Gallus gallus
Genetic Insert: Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1)
Vector Backbone Description: Backbone Size:6332; Vector Backbone:pMT; Vector Types:Bacterial Expression, zebrafish biotagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28402863
Proper citation: RRID:Addgene_80068 Copy
Species: Gallus gallus
Genetic Insert: Fibroblast growth factor receptor 1
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pCIG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807879
Proper citation: RRID:Addgene_80431 Copy
Species: Gallus gallus
Genetic Insert: VcnTS-E1015A
Vector Backbone Description: Backbone Size:5352; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38097542
Proper citation: RRID:Addgene_213415 Copy
Species: Gallus gallus
Genetic Insert: VcnTS-E1015A-E1021A
Vector Backbone Description: Backbone Size:5352; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38097542
Proper citation: RRID:Addgene_213417 Copy
Species: Gallus gallus
Genetic Insert: ASAP3-R3
Vector Backbone Description: Backbone Size:6103; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35256624
Comments: Please note that the Addgene NGS result contains ambiguous bases in the CAG promoter due to its GC-rich nature. Addgene's maps are automatically annotated by SnapGene. The mCyRFP3 fluorophore may be mis-annotated since not all variants are included on the common list of features. Please see the Annotated Depositor Map in the Supplemental Documents section for reference.
Proper citation: RRID:Addgene_193323 Copy
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