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Species: Rattus norvegicus
Genetic Insert: Erythropoietin Receptor
Vector Backbone Description: Vector Backbone:pCWXPGR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114286 Copy
Species: Rattus norvegicus
Genetic Insert: Erythropoietin
Vector Backbone Description: Vector Backbone:pCWXPGR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114290 Copy
Species: Rattus norvegicus
Genetic Insert: IRS-3
Vector Backbone Description: Backbone Marker:pBABE-hygro is available at Addgene; Backbone Size:5558; Vector Backbone:pBABE hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11113178
Comments: Rat IRS-3 was generated by PCR from the rat genomic DNA sequence. The intron was removed by PCR overlap extension using the following two pairs of primers: 5'-CGTGGATCCGCGATGAAGCCTGCAGGTACG-3' (sense) plus 5'-CTTGGGGGCTGAAACCCATGTTTGCTGGGCA-3' (antisense) and 5'-TGCCCAGCAAACATGGGTTTCAGCCCCCAAG-3' (sense) plus 5'-GCTGTCGACGTTCTAGAACTTGATGCTG-3' (antisense). BamHI and SalI sites were introduced at the 5' and 3' ends, respectively, of the coding sequence of IRS-3 by PCR. The subsequently generated coding sequence of rat IRS-3 was ligated into BamHI and SalI sites in pBABE-hygro.
Proper citation: RRID:Addgene_11450 Copy
Species: Rattus norvegicus
Genetic Insert: Cx26
Vector Backbone Description: Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26265468
Proper citation: RRID:Addgene_69016 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26265468
Comments: Addition of a fluorescent protein tag to the carboxyl-terminus of Cx43 has been shown to eliminate or attenuate interactions with ZO-1 and Occludin in cell culture. Other binding interactions and channel gating properties may be affected.
Proper citation: RRID:Addgene_69023 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Vector Backbone:EGFP-C1; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26265468
Comments: Note all connexins tested that have a fluorescent protein fused to the connexin amino-terminus do not form functional channels. This plasmid forms gap junction plaque structures but does not produce intercellular coupling.
Proper citation: RRID:Addgene_69020 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26265468
Comments: Note all connexins tested that have a fluorescent protein fused to the connexin amino-terminus do not form functional channels. This plasmid forms gap junction plaque structures but does not produce intercellular coupling.
Proper citation: RRID:Addgene_69025 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Backbone Marker:Fuhlbrigge, et al 1998, PMID 3261013; Vector Backbone:pSFFV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7586229
Comments: This plasmid was provided by Joseph Stains (University of Maryland School of Medicine), who received it from Thomas Steinberg (Washington University).
Proper citation: RRID:Addgene_69442 Copy
Species: Rattus norvegicus
Genetic Insert: paGFP-Omp25
Vector Backbone Description: Backbone Marker:Jason Moffat; Backbone Size:7327; Vector Backbone:pLJM2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25837514
Proper citation: RRID:Addgene_69598 Copy
Species: Rattus norvegicus
Genetic Insert: C2AB-linker-SNAP-25_C
Vector Backbone Description: Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26280336
Proper citation: RRID:Addgene_70057 Copy
Species: Rattus norvegicus
Genetic Insert: pHoenix
Vector Backbone Description: Backbone Size:8400; Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26551543
Proper citation: RRID:Addgene_70111 Copy
Species: Rattus norvegicus
Genetic Insert: GAT
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12223478
Proper citation: RRID:Addgene_70107 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95 beta isoform
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16815335
Comments: Map: FUGW (w/o PacI)-HinDIII-SalI-XbaI-(p95b)-KpnI-SacII-PspOMI-SmaI-BamHI-(GFP)-BglII-BsrBI-EcoRV-HinDIII-ClaI
Psd95 shRNA targeting sequence GGACATCCAGGCACACAAG was changed to GGATATTCAAGCGCATAAG to make it resistant to the shRNA sequence.
Proper citation: RRID:Addgene_74015 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95 shRNA
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16815335
Comments: Map: FH95pUp95GW-SalI-XbaI-(p97a)-BamHI-(linker)-(EGFP)-BglII-BsrBI-EcoRV-HinDIII-ClaI
Proper citation: RRID:Addgene_74019 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18215622
Comments: Map: FUGW-HinDIII-SalI-XbaI -(P95Q15AL460P)
Proper citation: RRID:Addgene_74033 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18215622
Comments: Map: FHUGW-(pUb)-HinDIII-SalI-XbaI-(P95 L460P)-XhoI-BsrGI (in EGFP)-NheI-XhoI-EcoRV-HinDIII-ClaI
Proper citation: RRID:Addgene_74032 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18215622
Comments: Map: FHUGW-(pUb)-HinDIII-SalI-XbaI-(P95 Q15AD45_64)-XhoI-BsrGI (in EGFP)-NheI-XhoI-EcoRV-HinDIII-ClaI
Proper citation: RRID:Addgene_74031 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18215622
Comments: Map: FUGW-HinDIII-SalI-XbaI-(p95Q15AdSK)-KpnI-PspOMI-SmaI-BamHI-(SGGlinker)-NcoI-(eGFP)-NheI-XhoI-EcoRV-HinDIII-ClaI
Proper citation: RRID:Addgene_74030 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18215622
Comments: Map: FHUGW-(pUb)-HinDIII-SalI-XbaI-(P95)-KpnI-ApaI-SmaI-BamHI-(GFPpren-BsrGI-)-BsrBI-EcoRV-HinDIII-ClaI
Proper citation: RRID:Addgene_74034 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19081375
Comments: Map: FHUGW-(pUb)-HinDIII-SalI-XbaI-(p95S73A)
Proper citation: RRID:Addgene_74039 Copy
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