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http://www.addgene.org/44362

Species: Homo sapiens
Genetic Insert: hM4D(Gi)-mCherry
Vector Backbone Description: Backbone Size:4818; Vector Backbone:pAAV; Vector Types:AAV, Adeno Associated Viral Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21364278
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.

Proper citation: RRID:Addgene_44362 Copy   

•   Source: Addgene

http://www.addgene.org/44471

Species: Mus musculus
Genetic Insert: Notch intracellular domain
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6300; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21131365

Proper citation: RRID:Addgene_44471 Copy   

•   Source: Addgene

http://www.addgene.org/44463

Species: Synthetic
Genetic Insert: QQR ZFN
Vector Backbone Description: Backbone Size:13812; Vector Backbone:pHEX2; Vector Types:Plant Transformation; Bacterial Resistance:Spectinomycin
Comments: QQR ZFN reference: Even-Faitelson L, Samach A, Melamed-Bessudo C, Avivi-Ragolsky N, Levy AA (2011) Localized egg-cell expression of effector proteins for targeted modification of the Arabidopsis genome. The Plant Journal 68 (5):929-937. doi:10.1111/j.1365-313X.2011.04741.x Please refer to the references provided below for matters relating to ownership of pHEX2 for anything other than academic use. pHEX2 reference: Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1 (1):13. doi:10.1186/1746-4811-1-13 pART27 (used to derive pHEX2) reference: Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203-1207

Proper citation: RRID:Addgene_44463 Copy   

•   Source: Addgene

http://www.addgene.org/44464

Species: Synthetic
Genetic Insert: AtCRU3 T494 TALENs
Vector Backbone Description: Backbone Size:13812; Vector Backbone:pHEX2; Vector Types:Plant Transformation; Bacterial Resistance:Spectinomycin
Comments: pZHY013 reference: Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, Voytas DF (2012) TALENs enable efficient plant genome engineering. Plant Physiology. doi:10.1104/pp.112.205179 Please refer to the references provided below for matters relating to ownership of pHEX2 for anything other than academic use. pHEX2 reference: Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1 (1):13. doi:10.1186/1746-4811-1-13 pART27 (used to derive pHEX2) reference: Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203-1207

Proper citation: RRID:Addgene_44464 Copy   

•   Source: Addgene

http://www.addgene.org/44466

Genetic Insert: A luciferase gene disrupted by a QQR ZFN target site and a frame-shift mutation
Vector Backbone Description: Backbone Size:5549; Vector Backbone:pGreenII 0579-1 (35S:: LUC); Vector Types:Luciferase, Plant Transformation; Bacterial Resistance:Kanamycin

Proper citation: RRID:Addgene_44466 Copy   

•   Source: Addgene

http://www.addgene.org/44456

Genetic Insert: pTEt-Bxb1, pBAD-TP901
Vector Backbone Description: Vector Backbone:J64100; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23539178

Proper citation: RRID:Addgene_44456 Copy   

•   Source: Addgene

http://www.addgene.org/44448

Vector Backbone Description: Vector Backbone:pHC115; Vector Types:Bacterial Expression, RK2, pMB1; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23648175

Proper citation: RRID:Addgene_44448 Copy   

•   Source: Addgene

http://www.addgene.org/44441

Species: Synthetic
Genetic Insert: Horseradish peroxidase fused to the transmembrane domain of PDGFR
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23371551
Comments: This construct has the Ig k-chain leader sequence on the N-terminus, which directs the protein to the secretory pathway. HRP faces the extracellular side of the plasma membrane.

Proper citation: RRID:Addgene_44441 Copy   

•   Source: Addgene

http://www.addgene.org/44447

Vector Backbone Description: Vector Backbone:pHC115; Vector Types:Bacterial Expression, RK2, pMB1; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23648175

Proper citation: RRID:Addgene_44447 Copy   

•   Source: Addgene

http://www.addgene.org/44446

Species: Homo sapiens
Genetic Insert: TFEB
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22692423

Proper citation: RRID:Addgene_44446 Copy   

•   Source: Addgene

http://www.addgene.org/44556

Species: Homo sapiens
Genetic Insert: MAVS-Fis1 chimeric protein
Vector Backbone Description: Backbone Size:6600; Vector Backbone:pMSCV2.2 IRES GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20451243

Proper citation: RRID:Addgene_44556 Copy   

•   Source: Addgene

http://www.addgene.org/44652

Species: Homo sapiens
Genetic Insert: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22823520

Proper citation: RRID:Addgene_44652 Copy   

•   Source: Addgene

http://www.addgene.org/44654

Species: Homo sapiens
Genetic Insert: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22823520

Proper citation: RRID:Addgene_44654 Copy   

•   Source: Addgene

http://www.addgene.org/44640

Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pFA6a-link; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.

Proper citation: RRID:Addgene_44640 Copy   

•   Source: Addgene

http://www.addgene.org/44642

Genetic Insert: mKO1
Vector Backbone Description: Vector Backbone:pFA6a-link; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.

Proper citation: RRID:Addgene_44642 Copy   

•   Source: Addgene

http://www.addgene.org/44595

Species: Mus musculus
Genetic Insert: BRCA1
Vector Backbone Description: Backbone Marker:Robert Weinberg (Addgene plasmid #8453); Backbone Size:7032; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23389450
Comments: Observed knockdown = 85%

Proper citation: RRID:Addgene_44595 Copy   

•   Source: Addgene

http://www.addgene.org/44630

Species: Mus musculus
Genetic Insert: Gfi1
Vector Backbone Description: Backbone Marker:Warren Pear lab; Backbone Size:6500; Vector Backbone:NGFR (Addgene plasmid 27489); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23444353

Proper citation: RRID:Addgene_44630 Copy   

•   Source: Addgene

http://www.addgene.org/44627

Species: Mus musculus
Genetic Insert: CEBPalpha
Vector Backbone Description: Backbone Marker:Warren Pear lab; Backbone Size:6500; Vector Backbone:NGFR (Addgene plasmid 27489); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23444353

Proper citation: RRID:Addgene_44627 Copy   

•   Source: Addgene

http://www.addgene.org/44571

Species: Homo sapiens
Genetic Insert: KRAS 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4281; Vector Backbone:pGL4.75; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: A substitute for pGL3-KRAS LCS6m in Chin et al., 2008.

Proper citation: RRID:Addgene_44571 Copy   

•   Source: Addgene

http://www.addgene.org/44561

Vector Backbone Description: Vector Backbone:pVV16, pSE100, pMC1m; Vector Types:Bacterial Expression, E.coli-mycobacteria shuttle vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23315736

Proper citation: RRID:Addgene_44561 Copy   

•   Source: Addgene