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Species: Plumbago zeylanica
Genetic Insert: PzPKS2
Vector Backbone Description: Backbone Size:3382; Vector Backbone:MoClo adapted pGAPZ-vector; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38457503
Proper citation: RRID:Addgene_219765 Copy
Species: Homo sapiens
Genetic Insert: PLD4
Vector Backbone Description: Backbone Size:1900; Vector Backbone:SuExp; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:38537643
Comments: Please visit https://doi.org/10.1101/2023.11.20.567917 for bioRxiv preprint.
Proper citation: RRID:Addgene_201244 Copy
Species: E. coli
Genetic Insert: MG1655-C321 mutS+, λ-, Δ(ybhB-bioAB)::zeoR, ΔprfA, ΔserB
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:36280721
Comments: MG1655-C321 -- All 321 UAG codons changed to UAA.
mutS+ -- Restores normal spontaneous mutation frequency.
(λ-) – Deleted Lambda Phage operon to allow growth at 37C.
Δ(ybhB-bioAB)::zeoR – Deletion of biotin biosynthetic operon using Zeocin cassette; strain is d-biotin auxotroph.
ΔprfA – Deletion of polypeptide release factor 1 (RF1) to prevent translation termination at UAG codons.
ΔserB – Deletion of phosphoserine phosphatase to provide intracellular source of phosphoserine; strain is Serine auxotroph.
The genome sequence of a similar strain (C321.deltaA) was deposited at NCBI (accession # CP006698.1)
Proper citation: RRID:Addgene_192872 Copy
Species: firefly
Genetic Insert: NFAT-Luciferase
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:4249; Vector Backbone:pSELECT-GFPzeo-mcs; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35173258
Proper citation: RRID:Addgene_180377 Copy
Genetic Insert: OsTIR1
Vector Backbone Description: Backbone Marker:Näätsaari et al., 2012; Vector Backbone:pPpT4; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:36043049
Proper citation: RRID:Addgene_189727 Copy
Species: Other
Genetic Insert: OsTIR1
Vector Backbone Description: Backbone Marker:Näätsaari et al., 2012; Vector Backbone:pPpT4; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:36043049
Proper citation: RRID:Addgene_189724 Copy
Vector Backbone Description: Backbone Marker:Ilya B. Tikh and James C. Samuelson; Backbone Size:4207; Vector Backbone:pDEL; Vector Types:Synthetic Biology; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35377874
Comments: - The destination strain should be recA+ and not be able to maintain R6K plasmid
- Zeocin is used to select colonies with the integrated vector.
- The rhaBAD and lac promoters can be induced with 0.2% rhamnose and 0.5mM IPTG
Proper citation: RRID:Addgene_155362 Copy
Vector Backbone Description: Backbone Marker:ExpreS2ion Biotechnologies; Backbone Size:3162; Vector Backbone:pExpreS2-1; Vector Types:Insect Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35600892
Comments: This plasmid is designed for use with the ExpreS2 Platform from ExpreS2ion Biotechnologies. It is intended to be used with the ExpreS2ion cells and media which can be obtained directly from the ExpreS2ion website.
Proper citation: RRID:Addgene_175443 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184321 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184301 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184306 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184305 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184302 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184303 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184316 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184320 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184324 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184299 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184300 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184304 Copy
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