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peSCBE3-NG-HF1
 
Resource Report
Resource Website 		RRID:Addgene_218164 escbe3-NG-HF1 S. coelicolor (bacteria) Apramycin PMID:38971862 Another insert : a programmable sgRNA cloning cassette Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin Mutation in the portion of deaminase hAPOBEC3A : Y130F, mutations in the portion of SpCas9 : D10A / N497A / R661A / Q695A / Q926A / L1111R / D1135V / G1218R / E1219F / A1322R / R1335V / T1337R. 2026-08-15 01:29:57 0
peSCBE3-NG-Hypa
 
Resource Report
Resource Website 		RRID:Addgene_218165 escbe3-NG-Hypa S. coelicolor (bacteria) Apramycin PMID:38971862 Another insert : a programmable sgRNA cloning cassette Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin Mutation in the portion of deaminase hAPOBEC3A : Y130F, mutations in the portion of SpCas9 : D10A / N692A / M694A / Q695A / H698A / L1111R / D1135V / G1218R / E1219F / A1322R / R1335V / T1337R. 2026-08-15 01:29:56 0
pSCBE3-NG-HF1-Hypa
 
Resource Report
Resource Website 		RRID:Addgene_218162 scbe3-NG-HF1-Hypa S. coelicolor (bacteria) Apramycin PMID:38971862 Another insert : a programmable sgRNA cloning cassette Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin Mutations in the portion of SpCas9 : D10A / N497A / R661A / N692A / M694A / Q695A / H698A / Q926A / L1111R / D1135V / G1218R / E1219F / A1322R / R1335V / T1337R. 2026-08-15 01:29:56 0
pSCBE3-Hypa
 
Resource Report
Resource Website 		RRID:Addgene_218157 scbe3-Hypa S. coelicolor (bacteria) Apramycin PMID:38971862 Another insert : a programmable sgRNA cloning cassette Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin Mutations in the portion of SpCas9 : D10A / N692A / M694A / Q695A / H698A. 2026-08-15 01:29:56 0
pSCBE3-HF1-Hypa
 
Resource Report
Resource Website 		RRID:Addgene_218158 scbe3-HF1-Hypa S. coelicolor (bacteria) Apramycin PMID:38971862 Another insert : a programmable sgRNA cloning cassette Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin Mutations in the portion of SpCas9 : D10A / N497A / R661A / N692A / M694A / Q695A / H698A / Q926A. 2026-08-15 01:29:57 0
pSCBE3-NG
 
Resource Report
Resource Website 		RRID:Addgene_218159 scbe3-NG S. coelicolor (bacteria) Apramycin PMID:38971862 Another insert : a programmable sgRNA cloning cassette Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin Mutations in the portion of SpCas9 : D10A / L1111R / D1135V / G1218R / E1219F / A1322R / R1335V / T1337R. 2026-08-15 01:29:56 0
peSCBE3-NG-HF1-Hypa
 
Resource Report
Resource Website 		RRID:Addgene_218166 escbe3-NG-HF1-Hypa S. coelicolor (bacteria) Apramycin PMID:38971862 Another insert : a programmable sgRNA cloning cassette Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, sgRNA transcription; Bacterial Resistance:Apramycin Mutation in the portion of deaminase hAPOBEC3A : Y130F, mutations in the portion of SpCas9 : D10A / N497A / R661A / N692A / M694A / Q695A / H698A / Q926A / L1111R / D1135V / G1218R / E1219F / A1322R / R1335V / T1337R. 2026-08-15 01:29:56 0
pMApra-Ptet1
 
Resource Report
Resource Website 		RRID:Addgene_218360 Apramycin PMID:39162403 Gebhardt lab ID # pMJG598. Vector Backbone:pMMB207; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-08-15 01:29:56 0
pWH1266-Apra-sgRNA
 
Resource Report
Resource Website 		RRID:Addgene_218372 sgRNA expression cassette Synthetic Apramycin PMID:39162403 Gebhardt lab ID # pMJG440. 5' Cloning Site: SpeI (Not Destroyed), 3' Cloning Site: ApaI (Not Destroyed). Backbone Size:9200; Vector Backbone:pWH1266; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin 2026-08-15 01:29:58 0
E. coli MG1655-Landing Pad-Clone 3
 
Resource Report
Resource Website 		RRID:Addgene_200007 Landing Pad Strain Synthetic Apramycin PMID:35366417 Vector Backbone:Custom; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-08-15 01:28:38 0
pJMP3664
 
Resource Report
Resource Website 		RRID:Addgene_222350 Apramycin PMID:39302127 Shuttle vector that replicates in A. baumannii and E. coli; replicates in common cloning strains such as DH10B. Vector Backbone:p15A and pWH1266; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-08-15 01:30:12 0
pJMP3666
 
Resource Report
Resource Website 		RRID:Addgene_222352 sfGFP Derived from Aequorea victoria. Apramycin PMID:39302127 Shuttle vector that replicates in A. baumannii and E. coli; replicates in common cloning strains such as DH10B. Vector Backbone:p15A and pWH1266; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-08-15 01:30:16 0
pJMP12049
 
Resource Report
Resource Website 		RRID:Addgene_222355 sfGFP Derived from Aequorea victoria. Apramycin PMID:39302127 Tn7 expression vector. Vector Backbone:R6Kγ; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-08-15 01:30:16 0
pCRISPR-mcBEST
 
Resource Report
Resource Website 		RRID:Addgene_209416 Codon optimized APOBEC1-nCas9-UGI fusion protein Synthetic Apramycin PMID:37402223 Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin 2026-08-15 01:28:27 0
pCRISPR-Cas3
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_209427 codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa Synthetic Apramycin PMID:40138716 Please visit https://doi.org/10.1101/2023.05.09.539971 for bioRxiv preprint. The used type I-C CRISPR system was first described by: Csörgő, B., León, L.M., Chau-Ly, I.J. et al. A compact Cascade–Cas3 system for targeted genome engineering. Nat Methods 17, 1183–1190 (2020). https://doi.org/10.1038/s41592-020-00980-w Plasmid contains an IS prokaryotic transposable element that does not affect plasmid function. The sequences used in pCRISPR-Cas3 were obtained via gene synthesis following codon optimization for S. coelicolor. Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin 2026-08-15 01:29:10 1
pCRISPR-cBEST-v2-SF14P
 
Resource Report
Resource Website 		RRID:Addgene_209448 Codon optimized APOBEC1-nCas9-UGI fusion protein Synthetic Apramycin PMID:38340355 Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin 2026-08-15 01:29:10 0
pL99
 
Resource Report
Resource Website 		RRID:Addgene_78577 Apramycin GenBank reference sequence JN636796 for NitR Vector Backbone:pIJ8668; Vector Types:Bacterial Expression, Streptomyces expression; Bacterial Resistance:Apramycin 2026-08-15 01:20:25 0
Pooled sgRNA Libraries Set 1 for Pseudomonas aeruginosa PA14
 
Resource Report
Resource Website 		RRID:Addgene_234855 Apramycin PMID:40595632 Backbone Marker:Addgene Plasmid #226432; Vector Backbone:pCRISPRi-ccdB; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-08-15 01:33:00 0
Reckleen_1_Cas9
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_233456 Level 2 RECKLEEN plasmid Other Apramycin PMID:41168338 Please visit https://doi.org/10.21203/rs.3.rs-5859838/v1 for preprint. Vector Backbone:Custom built using Marburg Collection; Vector Types:CRISPR; Bacterial Resistance:Apramycin 2026-08-15 01:34:04 1
pSEVAb897
 
Resource Report
Resource Website 		RRID:Addgene_217505 superfolder green fluorescent protein (sfGFP) Aequorea victoria Apramycin PMID:40471122 Vector Backbone:pSEVAb89; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F, M153T, V163A, I171V, and A206V 2026-08-15 01:33:26 0

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