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Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:20967238
Comments: For cloning taq polymerase-amplified PCR products, digest vector with Eam1105I, purify, ligate with PCR product, and use to transform DH5-alpha cells (or strain of choice). dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26536 Copy
Species: Oryza sativa
Genetic Insert: OsMIR390-B/c
Vector Backbone Description: Backbone Size:10145; Vector Backbone:pMDC32; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25809382
Proper citation: RRID:Addgene_61467 Copy
Species: Oryza sativa
Genetic Insert: OsMIR390-B/c
Vector Backbone Description: Backbone Size:2592; Vector Backbone:pENTR; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25809382
Proper citation: RRID:Addgene_61468 Copy
Species: Aequorea victoria
Genetic Insert: enhanced cyan fluorescent protein
Vector Backbone Description: Backbone Marker:Cambia, Canberra, Australia; Backbone Size:6000; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25390188
Proper citation: RRID:Addgene_61647 Copy
Species: Aequorea victoria
Genetic Insert: enhanced green fluorescent protein
Vector Backbone Description: Backbone Marker:Cambia, Canberra, Australia; Backbone Size:6000; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25390188
Proper citation: RRID:Addgene_61648 Copy
Species: Aequorea victoria
Genetic Insert: enhanced cyan fluorescent protein
Vector Backbone Description: Backbone Marker:Cambia, Canberra, Australia; Backbone Size:6000; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25390188
Proper citation: RRID:Addgene_61652 Copy
Species: Discosoma sp.
Genetic Insert: monomeric red fluorescent protein
Vector Backbone Description: Backbone Marker:Cambia, Canberra, Australia; Backbone Size:6000; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25390188
Proper citation: RRID:Addgene_61654 Copy
Species: Auquorea victoria
Genetic Insert: enhanced yellow fluorescent protein
Vector Backbone Description: Backbone Marker:Cambia, Canberra, Australia; Backbone Size:6000; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25390188
Proper citation: RRID:Addgene_61650 Copy
Species: Discosoma sp.
Genetic Insert: monomeric red fluorescent protein
Vector Backbone Description: Backbone Marker:Cambia, Canberra, Australia; Backbone Size:6000; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25390188
Proper citation: RRID:Addgene_61651 Copy
Vector Backbone Description: Backbone Size:11778; Vector Backbone:pEarleyGate-201; Vector Types:Plant Expression, BiFC assay; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:19843313
Proper citation: RRID:Addgene_61704 Copy
Vector Backbone Description: Backbone Size:8200; Vector Backbone:pDONR1K18ms; Vector Types:sucrose counter-selection allelic exchange vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:34032522
Proper citation: RRID:Addgene_72644 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:27084942
Comments: Please note that parental strain (MC4100) is streptomycin-resistant.
Proper citation: RRID:Addgene_69772 Copy
Species: Mus musculus
Genetic Insert: Ccl20 shRNA
Vector Backbone Description: Backbone Marker:Greg Hannon (PMID 16200065); Vector Backbone:pSM2C; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24415562
Proper citation: RRID:Addgene_65096 Copy
Vector Backbone Description: Vector Backbone:pK19mobsacB; Vector Types:; Bacterial Resistance:Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_68379 Copy
Vector Backbone Description: Backbone Marker:Yang lab; Backbone Size:11720; Vector Backbone:pKGO2, Addgene plasmid #63617; Vector Types:Fungal Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:15893252
Proper citation: RRID:Addgene_63618 Copy
Vector Backbone Description: Backbone Marker:Engler et al, 2014; Backbone Size:2000; Vector Backbone:pAGM1311; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153277 Copy
Vector Backbone Description: Backbone Marker:Engler et al, 2014; Backbone Size:2000; Vector Backbone:pAGM1311; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:33577114
Comments: It is recommended to screen DNA preps from a few different bacterial colonies by diagnostic digest to verify that a plasmid of the correct size has been isolated. Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153276 Copy
Vector Backbone Description: Backbone Marker:Engler et al, 2014; Backbone Size:2000; Vector Backbone:pAGM1311; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153279 Copy
Vector Backbone Description: Backbone Marker:Engler et al, 2014; Backbone Size:2000; Vector Backbone:pAGM1311; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153278 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141015 Copy
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