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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
RVD-TC8
 
Resource Report
Resource Website
RRID:Addgene_173399 NG-HD dimer RVD at Position8 Synthetic Tetracycline PMID:34480028 RVD is inserted into TA site of TetR T vector Vector Backbone:TetR T vector; Vector Types:Unspecified; Bacterial Resistance:Tetracycline no 2026-08-15 01:25:56 0
RVD-TC6
 
Resource Report
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RRID:Addgene_173397 NG-HD dimer RVD at Position6 Synthetic Tetracycline PMID:34480028 RVD is inserted into TA site of TetR T vector Vector Backbone:TetR T vector; Vector Types:Unspecified; Bacterial Resistance:Tetracycline no 2026-08-15 01:25:56 0
RVD-TC5
 
Resource Report
Resource Website
RRID:Addgene_173396 NG-HD dimer RVD at Position5 Synthetic Tetracycline PMID:34480028 RVD is inserted into TA site of TetR T vector Vector Backbone:TetR T vector; Vector Types:Unspecified; Bacterial Resistance:Tetracycline no 2026-08-15 01:25:56 0
RVD-TC3
 
Resource Report
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RRID:Addgene_173394 NG-HD dimer RVD at Position3 Synthetic Tetracycline PMID:34480028 RVD is inserted into TA site of TetR T vector Vector Backbone:TetR T vector; Vector Types:Unspecified; Bacterial Resistance:Tetracycline no 2026-08-15 01:25:56 0
RVD-TC2
 
Resource Report
Resource Website
RRID:Addgene_173393 NG-HD dimer RVD at Position2 Synthetic Tetracycline PMID:34480028 RVD is inserted into TA site of TetR T vector Vector Backbone:TetR T vector; Vector Types:Unspecified; Bacterial Resistance:Tetracycline no 2026-08-15 01:25:56 0
pMP220
 
Resource Report
Resource Website
RRID:Addgene_185921 Tetracycline PMID:24276795 the polylinker of pIC20H is broadly available and isn't covered by an MTA Backbone Marker:Spaink; Backbone Size:10400; Vector Backbone:pMP103; Vector Types:Bacterial Expression, IncP; Bacterial Resistance:Tetracycline 2026-08-15 01:27:17 0
pDT2
 
Resource Report
Resource Website
RRID:Addgene_199119 lambda PR' - repeat cassette - E. coli rpoB - lambda TR' Synthetic Tetracycline PMID:37406096 Vector Backbone:pFW11 Tet; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to introduce a second BsaI site 2026-08-15 01:27:19 0
pNDGG004
 
Resource Report
Resource Website
RRID:Addgene_231317 Tetracycline PMID:39919271 Vector Backbone:NA; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:32:04 0
US0 pyrF-/hisB-/tnaA-
 
Resource Report
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RRID:Addgene_228512 pyrF-/hisB-/tnaA- n/a Tetracycline PMID:39610115 Derived from US0 pyrF-/hisB- (Scot Wolfe, Addgene #12614). This strain has a deletion in both the hisB and pyrF genes (the bacterial homologs of HIS3 and URA3) and it contains a F' episome bearing the lacl^q repressor. This strain is also has a deletion in tnaA gene and does not produce indole, verified by Kovac's Reagent. Please visit https://doi.org/10.1101/2024.08.07.607023 for bioRxiv preprint. Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:Tetracycline 2026-08-15 01:31:50 0
pALS3-Ma-sfGFP-WT
 
Resource Report
Resource Website
RRID:Addgene_212119 SUMO-sfGFP-WT Tetracycline This plasmid encodes expression of a) sfGFP-WT, uninterrupted by TAG codons, and b) Methanomethylophilus alvus tRNA(6). It is to be paired with a plasmid that expresses M. alvus pyrrolysyl-tRNA synthetase (MaRS). This control plasmid is used during fluorescence-based selection and characterization of one or more MaRS mutants. It is distinguished from a previous Ma version by containing a tetracycline efflux marker. Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:30:28 0
pALS3-Ma-SUMO-sfGFP-WT
 
Resource Report
Resource Website
RRID:Addgene_212122 SUMO-sfGFP-WT Tetracycline This plasmid encodes expression of a) sumo-sfGFP, uninterrupted by TAG codons, and b) Methanomethylophilus alvus pyrrolysine tRNA(6). It is to be paired with a plasmid that expresses M. alvus pyrrolysyl-tRNA synthetase (MaRS). This pALS3 control plasmid is used during fluorescence-based selection and characterization of Ma RS mutants. The plasmid here is distinguished from a previous Ma version by the presence of a tetracycline efflux marker. Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:30:28 0
pALS3-Ma-SUMO-TAG35-sfGFP
 
Resource Report
Resource Website
RRID:Addgene_212120 SUMO-TAG35-sfGFP Tetracycline This plasmid encodes expression of a) SUMO-sfGFP with an amber (TAG) codon at E35 within the SUMO coding region and b) Methanomethylophilus alvus tRNA(6). It is to be paired with a plasmid that expresses an M. alvus pyrrolysyl-tRNA synthetase (MaRS). This plasmid is used during fluorescence-based selection and characterization of one or more MaRS mutants. It is distinguished from the previous Ma version by containing a tetracycline efflux marker. The SUMO linker is included in order to distance the TAG site from sfGFP. Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline E35TAG (within SUMO encoding sequence) 2026-08-15 01:30:28 0
pHBJT010
 
Resource Report
Resource Website
RRID:Addgene_225161 Tetracycline PMID:39244484 Low copy plasmid (pSC101 ori and repA), pUC18 MCS with FLAG epitope integrated at SmaI site, confers tetracycline resistance Backbone Size:4297; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:30:32 0
pHBJT032
 
Resource Report
Resource Website
RRID:Addgene_225178 Tetracycline PMID:39244484 pHBJT019 containing IPTG-inducible mCherry Backbone Size:4533; Vector Backbone:pHBJT019; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:30:33 0
pHBJT020
 
Resource Report
Resource Website
RRID:Addgene_225171 Tetracycline PMID:39244484 Low copy plasmid (pSC101 ori and repA), pUC18 MCS, confers tetracycline resistance Backbone Size:4532; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:30:32 0
pHBJT019
 
Resource Report
Resource Website
RRID:Addgene_225170 Tetracycline PMID:39244484 Low copy plasmid (pSC101 ori and repA), pUC19 MCS, confers tetracycline resistance Backbone Size:4533; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:30:32 0
pNDMS237
 
Resource Report
Resource Website
RRID:Addgene_248095 sfYFP Tetracycline Backbone Marker:Barney Geddes; Vector Backbone:pNDGG004 (Addgene #231317); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:34:43 0
pNDMS239
 
Resource Report
Resource Website
RRID:Addgene_248096 mScarlet-I Tetracycline Backbone Marker:Barney Geddes; Vector Backbone:pNDGG004 (Addgene #231317); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:34:44 0
pRK415-mNeonGreen (constitutive expression)
 
Resource Report
Resource Website
RRID:Addgene_248204 mNeonGreen Synthetic Tetracycline Backbone Marker:Nova Lifetech Inc.; Backbone Size:10682; Vector Backbone:pRK415; Vector Types:Bacterial Expression, Synthetic Biology, very low copy; Bacterial Resistance:Tetracycline 2026-08-15 01:35:18 0
pGS1T-PylRSC6a-MmtRNAPylCUA
 
Resource Report
Resource Website
RRID:Addgene_251555 PylRS-C6a Synthetic Tetracycline PMID:41974833 Please visit https://doi.org/10.1101/2025.02.09.636911 for bioRxiv preprint. Backbone Marker:in-house design and optimization; Vector Backbone:pGS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:35:19 0

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