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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pICE-HA-NLS-I-PpoI
 
Resource Report
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1+ mentions
RRID:Addgene_46963 NLS-I-PpoI Physarum polycephalum Ampicillin PMID:23897892 Backbone Size:5044; Vector Backbone:pICE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:37 3
pEGFP-C1-FLAG
 
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1+ mentions
RRID:Addgene_46956 FLAG tag Synthetic Kanamycin PMID:23897892 Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:15:37 9
pEGFP-C1-FLAG-Ku70
 
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10+ mentions
RRID:Addgene_46957 Ku70 Homo sapiens Kanamycin PMID:23897892 Backbone Size:4766; Vector Backbone:pEGFP-C1-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:15:36 13
p52sheLL
 
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1+ mentions
RRID:Addgene_46950 HPV52L1+L2 Homo sapiens Ampicillin PMID:18360909 Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:36 2
pINDUCER21 (ORF-EG)
 
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10+ mentions
RRID:Addgene_46948 Chloramphenicol and Ampicillin PMID:21307310 Backbone Marker:Dr. Richard Mulligan; Backbone Size:4874; Vector Backbone:pHAGE-CAGS-W; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:15:36 33
pCIneoEGFP
 
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1+ mentions
RRID:Addgene_46949 GFP Aequorea victoria Ampicillin PMID:18228512 Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCIneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:37 5
pIRES-Ap2a2-FKBP
 
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1+ mentions
RRID:Addgene_46944 Ap2a2-FKBP Homo sapiens Ampicillin PMID:20159602 Backbone Marker:Clontech; Vector Backbone:pIRESneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:36 1
pEYFP-Mitotrap
 
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1+ mentions
RRID:Addgene_46942 Mitotrap Homo sapiens Kanamycin PMID:20159602 Backbone Marker:Clontech; Vector Backbone:pEYFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:15:36 4
pcDNA-f:PGC1-3D
 
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1+ mentions
RRID:Addgene_47 PGC-1a Mus musculus Ampicillin PMID:14744933 Please see Addgene plasmid 1026 (pcDNA-f:PGC1) for wildtype sequence and map. Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Amino acids 262, 265, and 298 have been changed to aspartic acid 2026-08-15 01:15:37 6
5HRE/GFP
 
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10+ mentions
RRID:Addgene_46926 5X HRE of VEGF Homo sapiens Ampicillin PMID:11774035 The 35-bp human VEGF HRE sequence is: CCACAGTGCATACGTGGGCTCCAACAGGTCCTCTT The d2EGFP fragment was amplified from a Clontech (Palo Alto, CA) vector and encodes a destabilized red- shifted variant of wild-type GFP with an excitation maximum of 488 nm, an emission maximum of 507 nm, and a half-life of approximately 2 hours. Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pEF/myc/cyto; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin five copies of a 35-bp fragment from the hypoxia-responsive element (HRE) of the human VEGF gene and a human cytomegalovirus minimal promoter 2026-08-15 01:15:37 24
YFP-Parkin C431N
 
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1+ mentions
RRID:Addgene_46924 Park2 Homo sapiens Kanamycin PMID:23319602 Backbone Marker:Clontech; Backbone Size:4698; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin changed cysteine 431 to asparagine 2026-08-15 01:15:36 2
LZRSMS-GFP-hFJX1-Flag
 
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1+ mentions
RRID:Addgene_46929 FJX1 Homo sapiens Ampicillin Backbone Size:12800; Vector Backbone:LZRS-MS-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:15:36 1
pMA3288
 
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1+ mentions
RRID:Addgene_46885 Uracil-N-glycosylase WT Homo sapiens Ampicillin PMID:23721969 Backbone Marker:Homemade; Backbone Size:6686; Vector Backbone:pMA2780; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:15:36 1
pMSP3535
 
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1+ mentions
RRID:Addgene_46886 Erythromycin PMID:10964628 This plasmid utilizes the NIsin Controlled Expression (NICE) system. NICE® is a registered trademark of NIZO food research BV, P.O.Box 20, 6710 BA Ede, The Netherlands. pMSP3535 contains the Nisin-inducible PnisA promoter, and the pAMB1 replicon for expression in gram-positive bacteria. For protein expression in E. faecalis, induce with 10-25 ng/mL nisin (Sigma, N-5764). See associated article for instructions on how to make nisin solution. Note: Nucleotide sequence encoding the signal sequence and one-third of the mature NisA (nisin) peptide remains associated with the PnisA promoter. The depositing laboratory recommends adding of a Stop codon to the 5' end of inserted sequences to avoid unintentional gene fusions. Addgene's quality control sequencing finds discrepancies with the depositor's provided sequencew which includes a mutation in RepE- F229L. This however is thought to not affect plasmid function. Backbone Size:8353; Vector Backbone:pMSP3535; Vector Types:Bacterial Expression, Nisin-inducible expression; Gram-positive Bacterial Shuttle Vector; Bacterial Resistance:Erythromycin 2026-08-15 01:15:36 6
pMSP3535VA
 
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1+ mentions
RRID:Addgene_46887 Kanamycin PMID:10964628 This plasmid utilizes the NIsin Controlled Expression (NICE) system. NICE® is a registered trademark of NIZO food research BV, P.O.Box 20, 6710 BA Ede, The Netherlands. pMSP3535VA contains the Nisin-inducible PnisA promoter, and the pVA380-1 replicon for expression in gram-positive bacteria. For protein expression in E. faecalis, induce with 10-25 ng/mL nisin (Sigma, N-5764). See associated article for instructions on how to make nisin solution. Note: Nucleotide sequence encoding the signal sequence and one-third of the mature NisA (nisin) peptide remains associated with the PnisA promoter. The depositing laboratory recommends adding of a Stop codon to the 5' end of inserted sequences to avoid unintentional gene fusions. Note: The theoretical full sequence uploaded here is NOT fully accurate - the NheI site at position 4414 has been lost, along with approximately 500bp of sequence in this region (compare the depositor's uploaded maps). The actual size of this plasmid is 8278 as listed here. The depositing lab has not resequenced the region in question, but the discrepancy does not affect plasmid function. Note: This plasmid has been shown to accumulate deletions when replicated in E. coli (or gram-negative bacteria). Screening transformants by restriction digest is recommended. Addgene's quality control sequencing finds discrepancies with the depositor's provided sequence, but they are not thought to affect plasmid function. Backbone Size:8278; Vector Backbone:pMSP3535VA; Vector Types:Bacterial Expression, Nisin-inducible expression; Gram-positive Bacterial Shuttle Vector; Bacterial Resistance:Kanamycin 2026-08-15 01:15:36 1
pTDH3-dCas9
 
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1+ mentions
RRID:Addgene_46920 dCas9 Ampicillin PMID:23849981 The Cas9 contains a N612K mutation that does not effect the function of the enzyme. Backbone Size:6900; Vector Backbone:pJED103; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:15:36 7
pU6-sgGFP-NT1
 
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1+ mentions
RRID:Addgene_46914 sgGFP-NT1 Ampicillin PMID:23849981 gRNA target sequence GAATAGCTCAGAGGCCGAGG Backbone Marker:Addgene; Backbone Size:8200; Vector Backbone:pSICO derivative; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:15:36 8
pU6-sgGAL4-1
 
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1+ mentions
RRID:Addgene_46915 sgGAL4-1 Ampicillin PMID:23849981 gRNA target sequence GTTGGAGCACTGTCCTCCGAACGT Backbone Marker:Addgene; Backbone Size:8200; Vector Backbone:pSICO derivative; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:15:36 2
pMA3211
 
Resource Report
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1+ mentions
RRID:Addgene_46879 Ampicillin PMID:22637478 Backbone Marker:Homemade; Backbone Size:6642; Vector Backbone:pMA3211; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:15:36 1
pMSCV-LTR-dCas9-p65AD-BFP
 
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1+ mentions
RRID:Addgene_46913 dCas9-p65AD-BFP fusion Homo sapiens Ampicillin PMID:23849981 Backbone Marker:Clontech; Backbone Size:6300; Vector Backbone:MSCV-puro; Vector Types:Mammalian Expression, Retroviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:15:37 2

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