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Species: Danio rerio
Genetic Insert: E1b:eGFP
Vector Backbone Description: Backbone Size:3873; Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: A. Emelyanov and S. Parinov. Mifepristone-inducible LexPR system to drive and control gene expression in transgenic zebrafish. Developmental Biology, 320(1):113– 121, 2008. ISSN 00121606. doi: 10.1016/j.ydbio.2008.04.042.
A. Emelyanov, Y. Gao, N. I. Naqvi, and S. Parinov. Trans-kingdom transposition of the maize Dissociation element. Genetics, 174(3):1095–1104, 2006. ISSN 00166731. doi: 10.1534/genetics.106.061184.
Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_102417 Copy
Species: Danio rerio
Genetic Insert: myelin basic protein
Vector Backbone Description: Backbone Size:4308; Vector Backbone:pZL1; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12112375
Proper citation: RRID:Addgene_103007 Copy
Species: Danio rerio
Genetic Insert: twhh promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCS-NBeta gal; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9314535
Comments: Insert: twhh promoter (5.2 Kb), nuclear Beta gal reporter (3 Kb)
Plasmid History:
Source: zebrafish.
Notes for Use:
Gives notochord-specific expression of Beta-gal.
Citation: Du et al., 1997, J. Cell Biology.
Proper citation: RRID:Addgene_17152 Copy
Species: Danio rerio
Genetic Insert: twhh promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:NA; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Insert: twhh promoter (5.2 Kb), rabbit Beta-globin intron 2 (IVS2, 640 bp), GFP (65C, 1Kb)
Plasmid History:
Source: zebrafish.
Notes for Use:
Gives both notochord and floor-plate specific expression of GFP, owing to having the intron present.
Proper citation: RRID:Addgene_17154 Copy
Species: Danio rerio
Genetic Insert: nacre promoter driving nacre cDNA
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10652270
Comments: Note for Use:
Description: Zebrafish nacre promoter driving nacre cDNA.
Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.
Proper citation: RRID:Addgene_17174 Copy
Species: Danio rerio
Genetic Insert: nacre promoter
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10652270
Comments: Description: zebrafish nacre promoter with all three LEF sites mutated, driving luciferase reporter. This is a control construct.
Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.
Proper citation: RRID:Addgene_17177 Copy
Species: Danio rerio
Genetic Insert: nacre promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10652270
Comments: Note for Use:
Description: zebrafish nacre promoter driving GFP. A 836-bp fragment including the first 10 amino acids of nacre was fused to GFP.
Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.
Proper citation: RRID:Addgene_17178 Copy
Species: Danio rerio
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28 b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Description: This is full length zebrafish beta catenin cDNA with a TEV protease site at the amino terminus following the HIS tag. Cloned into Sal1 and Not1 sites.
Reference: Made by Ken-Ichi Takemaru.
Proper citation: RRID:Addgene_17199 Copy
Species: Danio rerio
Genetic Insert: Sp5l promoter
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Comments: Description: a fragment of putative zebrafish Sp5l promoter - 1203bp upstream of the 1st Exon.
(as defined by the 27i2 clone found in the microarray screen) - plus 51bp of 5'UTR from Exon1 clone.
upstream of firefly luciferase in the pGL3 Basic promoter. 6 of the putative Tcf/Lef sites mutated.
(all introduce additional restriction sites); cloned by PCR-based in vitro synthesis from 28 overlapping.
oligos. cloned NdeI / HindIII to exchange wild-type fragment in pGL3 Sp5l P1203.
Species of Sequence Origin: zebrafish.
Proper citation: RRID:Addgene_17209 Copy
Species: Danio rerio
Genetic Insert: prolactinSS-PP1-mCitrine
Vector Backbone Description: Backbone Size:8740; Vector Backbone:pLenti 2nd gen; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34785668
Proper citation: RRID:Addgene_172472 Copy
Species: Danio rerio
Genetic Insert: -3.5ubb:plin2-tdtomato
Vector Backbone Description: Backbone Size:7011; Vector Backbone:pDestTol2pA2-Blasticidin; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34114952
Comments: Please note that the Addgene verified sequence identified S80R, A81S, E144D, I148F, and R415H mutations in PLIN2-tdTomato. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_172535 Copy
Species: Danio rerio
Genetic Insert: cct3-GFP
Vector Backbone Description: Backbone Marker:Joachim Berger; Backbone Size:6353; Vector Backbone:pDESTtol2pACrymCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320728
Proper citation: RRID:Addgene_172528 Copy
Species: Danio rerio
Genetic Insert: kcnj2a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173127 Copy
Species: Danio rerio
Genetic Insert: kcnj1b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-Topo; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173126 Copy
Species: Danio rerio
Genetic Insert: kcnj15
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-Topo; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173145 Copy
Species: Danio rerio
Genetic Insert: kcnj12a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173141 Copy
Species: Danio rerio
Genetic Insert: kcnj11l
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173140 Copy
Species: Danio rerio
Genetic Insert: kcnj10b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-Topo; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173138 Copy
Species: Danio rerio
Genetic Insert: kcnj10a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR-D-Topo; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173137 Copy
Species: Danio rerio
Genetic Insert: kcnj5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34558132
Comments: This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
Proper citation: RRID:Addgene_173132 Copy
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