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Species: Other
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166018 Copy
Species: Other
Genetic Insert: HCoV-229E S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166016 Copy
Species: Other
Genetic Insert: SARS-CoV-2 S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166010 Copy
Species: Other
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166020 Copy
Species: Other
Genetic Insert: 40XUAS , Luciferase
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166455 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_166235 Copy
Species: Other
Genetic Insert: Ty tag fused to mNeon
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_166237 Copy
Species: Other
Genetic Insert: HaloTag
Vector Backbone Description: Vector Backbone:pIRES_neo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852913
Proper citation: RRID:Addgene_166907 Copy
Species: Other
Genetic Insert: Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
Vector Backbone Description: Vector Backbone:pET-23b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26092728
Comments: Bacillus thuringiensis PI-PLC (UniProt P08954) cleaves GPI-anchored proteins off the surface of cells and can be used instead of the 97% identical, commercially available B. cereus enzyme (UniProt P14262).
Also see PubMed 30148347 (bacterial PI-PLC review).
Proper citation: RRID:Addgene_173808 Copy
Species: Other
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pE-FLP; Vector Types:Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34190535
Comments: Depositor confirms the S102Y and S279Y in Cre does not affect plasmid function.
The pE_Cre_lambdaCos plasmid can be packaged in lambda phages using the CY2120 cosmid packaging strain [Cronan 2013]. Lambda infection provides a rapid and efficient way to deliver the pE_Cre_lambdaCos plasmid.
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_173785 Copy
Species: Other
Genetic Insert: Staphylococcus aureus plc
Vector Backbone Description: Vector Backbone:pET-21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22390775
Comments: See UniProt ID P45723 for the mature, untagged sequence.
Also see PubMed 30148347 (bacterial PI-PLC review).
Proper citation: RRID:Addgene_173810 Copy
Species: Other
Genetic Insert: A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9
Vector Backbone Description: Backbone Marker:https://www.addgene.org/52961/; Vector Backbone:LentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173841 Copy
Species: Other
Genetic Insert: A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9
Vector Backbone Description: Backbone Marker:https://www.addgene.org/52961/; Vector Backbone:LentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173842 Copy
Species: Other
Genetic Insert: A gRNA targeting the dog Egfr gene and the cDNA of CRISPR-Cas9
Vector Backbone Description: Backbone Marker:https://www.addgene.org/52961/; Vector Backbone:LentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173844 Copy
Species: Other
Genetic Insert: A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
Vector Backbone Description: Backbone Marker:https://www.addgene.org/52961/; Vector Backbone:LentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173840 Copy
Species: Other
Genetic Insert: MiCy with mitochondria-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174177 Copy
Species: Other
Genetic Insert: mKate2 with mitochondria-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174178 Copy
Species: Other
Genetic Insert: eqFP650 with mitochondria-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174179 Copy
Species: Other
Genetic Insert: Venus with mitchondria-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174187 Copy
Species: Other
Genetic Insert: mCherry with mitchondria-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174188 Copy
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