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Species: Other
Genetic Insert: Right homology arm for targeting integration to chromosome IV:240042..241741
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Meant for the construction of integrative vectors (single- or multi-gene). Contains the appropriate YTK-compatible Golden Gate overhangs for a type 1 part. The necessary internal BsmBI sites to use this as a connector for multi-gene assembly have been added by PCR. Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125066 Copy
Species: Other
Genetic Insert: KmARS/CEN5
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 7 part. Equivalent to the ARS1/CEN sequence from strain ATCC 12424 Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125060 Copy
Species: Other
Genetic Insert: LAC4 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Promoter inducible by lactose and galactose. Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125052 Copy
Species: Other
Genetic Insert: KmARS7/CenD
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 7 part. Minimal centromeric sequence/ARS derived from Kluyveromyces marxianus DMKU3-1042. Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125059 Copy
Species: Other
Genetic Insert: ALD4 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125042 Copy
Species: Other
Genetic Insert: REV1 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125041 Copy
Species: Other
Genetic Insert: HSP104 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Promoter inducible by heat (37-42 degrees). Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125046 Copy
Species: Other
Genetic Insert: SSA2 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Promoter inducible by heat (37-42 degrees). Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125047 Copy
Species: Other
Genetic Insert: HHF1 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125044 Copy
Species: Other
Genetic Insert: FBA1 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125049 Copy
Species: Other
Genetic Insert: LexA
Vector Backbone Description: Backbone Marker:Koen Venken; Vector Backbone:pBS-KS-attB-1-2-PT-SA-SD-2; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25712101
Proper citation: RRID:Addgene_125222 Copy
Species: Other
Genetic Insert: GeneSwitch
Vector Backbone Description: Backbone Marker:Koen Venken; Vector Backbone:pBS-KS-attB-1-2-PT-SA-SD-2; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25712101
Proper citation: RRID:Addgene_125216 Copy
Species: Other
Genetic Insert: D. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2962; Vector Backbone:Unknown; Vector Types:Insect Expression, Gateway fly expression vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31092928
Proper citation: RRID:Addgene_125153 Copy
Species: Other
Genetic Insert: D. pseudoobscura peak #47 enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3078; Vector Backbone:Unknown; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31092928
Proper citation: RRID:Addgene_125151 Copy
Species: Other
Genetic Insert: CDA1-BE4max-NG
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332326
Comments: Please see https://benchling.com/s/seq-G8jxMvNS0GdjaatqGCZ1 for additional information.
Proper citation: RRID:Addgene_125612 Copy
Species: Other
Genetic Insert: evoCDA1-BE4max-NG
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332326
Comments: Please see https://benchling.com/s/seq-6U1OlYv3SFGTPZrYyUhM for additional information.
Proper citation: RRID:Addgene_125613 Copy
Species: Other
Genetic Insert: FERNY-BE4max-NG
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332326
Comments: Please see https://benchling.com/s/seq-Ics96qtTq9lyzSiUBnpS for additional information.
Proper citation: RRID:Addgene_125614 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31548381
Comments: Please visit https://www.biorxiv.org/content/10.1101/582403v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_125689 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spCas9, sgRNA casette
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Other
Defining Citation: PMID:25806970
Proper citation: RRID:Addgene_125686 Copy
Species: Other
Genetic Insert: shRFP
Vector Backbone Description: Backbone Size:8901; Vector Backbone:pLKO.1-puro; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30971823
Comments: Please visit https://www.biorxiv.org/content/10.1101/502070v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_125778 Copy
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