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Genetic Insert: 135(T2AG3)
Vector Backbone Description: Backbone Size:2400; Vector Backbone:pSP73; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8090736
Proper citation: RRID:Addgene_12402 Copy
Genetic Insert: Sty11
Vector Backbone Description: Backbone Size:2400; Vector Backbone:pSP73; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1537344
Comments: Insert used as probe for telomere blots
Proper citation: RRID:Addgene_12401 Copy
Genetic Insert: 270(T2AG3)
Vector Backbone Description: Backbone Size:2400; Vector Backbone:pSP73; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8090736
Proper citation: RRID:Addgene_12403 Copy
Species: Synthetic
Genetic Insert: Orange CaMBI 300
Vector Backbone Description: Backbone Size:6330; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30936501
Comments: Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor sequence for this section of the plasmid.
Proper citation: RRID:Addgene_124095 Copy
Species: Synthetic
Genetic Insert: Orange CaMBI 110
Vector Backbone Description: Backbone Size:6330; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30936501
Comments: The C-terminal copy of the CyOFP coding sequence is codon diversified to facilitate molecular biology procedures on Orange CaMBI and reduce the possibility of recombination between the two copies of the CyOFP coding sequence.
Proper citation: RRID:Addgene_124094 Copy
Species: Escherichia coli
Genetic Insert: Release factor 2
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11479568
Comments: It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.
Proper citation: RRID:Addgene_124130 Copy
Species: Escherichia coli
Genetic Insert: Ribosome recycling factor
Vector Backbone Description: Vector Backbone:pQE60; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11479568
Comments: It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.
Proper citation: RRID:Addgene_124132 Copy
Species: Escherichia coli
Genetic Insert: Methionyl-tRNA formyltransferase
Vector Backbone Description: Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11479568
Comments: It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.
Proper citation: RRID:Addgene_124133 Copy
Species: Mus musculus
Genetic Insert: mTSP1 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:PXP1 luciferase; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_12409 Copy
Species: Enterobacteria phage T7
Genetic Insert: T7 RNA polymerase
Vector Backbone Description: Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11479568
Comments: This plasmid is provided in NEB Stable cells from Addgene; however, the depositor originally cloned this plasmid in JM109 cells. It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.
Proper citation: RRID:Addgene_124138 Copy
Species: Mus musculus
Genetic Insert: TSP1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_12405 Copy
Species: Mus musculus
Genetic Insert: TSP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_12411 Copy
Species: Homo sapiens
Genetic Insert: TET1 - catalytic domain mutated
Vector Backbone Description: Backbone Size:6181; Vector Backbone:pEF1 alpha; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30698829
Comments: The two mutations listed before (H1672Y, D1674A) were introduced using the
QuickChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies).
Proper citation: RRID:Addgene_124083 Copy
Species: Escherichia coli
Genetic Insert: Tyrosyl-tRNA ligase
Vector Backbone Description: Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11479568
Comments: It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.
Proper citation: RRID:Addgene_124121 Copy
Species: Escherichia coli
Genetic Insert: Tryptophanyl-tRNA ligase
Vector Backbone Description: Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11479568
Comments: It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.
Proper citation: RRID:Addgene_124120 Copy
Species: Escherichia coli
Genetic Insert: Valyl-tRNA ligase
Vector Backbone Description: Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11479568
Comments: It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.
Proper citation: RRID:Addgene_124122 Copy
Species: Escherichia coli
Genetic Insert: Initiation factor 3
Vector Backbone Description: Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11479568
Comments: It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.
Proper citation: RRID:Addgene_124125 Copy
Species: Escherichia coli
Genetic Insert: Elongation factor Tu
Vector Backbone Description: Vector Backbone:pQE60; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11479568
Comments: It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.
Proper citation: RRID:Addgene_124127 Copy
Species: Escherichia coli
Genetic Insert: Elongation factor G
Vector Backbone Description: Vector Backbone:pQE60; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11479568
Comments: It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.
Proper citation: RRID:Addgene_124126 Copy
Species: Homo sapiens
Genetic Insert: TET1 catalytic domain
Vector Backbone Description: Backbone Size:6181; Vector Backbone:pEF1 alpha; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30698829
Proper citation: RRID:Addgene_124082 Copy
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