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Species: Synthetic
Genetic Insert: PUFb_p65HSF1
Vector Backbone Description: Vector Backbone:pAC90-pmax-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates and protocols
Proper citation: RRID:Addgene_73697 Copy
Species: Synthetic
Genetic Insert: LINXa3
Vector Backbone Description: Backbone Size:5940; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27089030
Proper citation: RRID:Addgene_73614 Copy
Species: Synthetic
Genetic Insert: ratiometric pHluorin2
Vector Backbone Description: Vector Backbone:PCR8; Vector Types:middle entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24973752
Comments: Ratiometric pHluorin2 was originally published in the following paper:
Mahon, M. (2011) pHluorin2: an enhanced, ratiometric, pH-sensitive green florescent protein. Advances in Bioscience and Biotechnology, 2, 132-137. doi: 10.4236/abb.2011.23021.
Proper citation: RRID:Addgene_73794 Copy
Species: Synthetic
Genetic Insert: dCas9-Mxi1
Vector Backbone Description: Backbone Marker:https://www.addgene.org/vector-database/3985/; Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26956608
Comments: Detailed benchling plasmid map available at: https://benchling.com/s/0WUKQB/edit
All annotations are available there. This is best reference to use for this plasmid. The genbank file included was exported from benchling for this plasmid.
Please note to clone in gRNAs you need to use Gibson Assembly. Gibson will chew back the NotI overhangs. Provide homology on either side of the cut site to clone. Generally we order 60mers with 20bp of homology to the promoter and to the terminator. For high efficiency amplify with primers that extend these homologies to 30-40bp. You can directly clone in single stranded oligo with 20bp overlaps using Gibson if you only need low efficiency. To do this use ~100x oligo to plasmid. For double stranded PCR product, use ratios between to 2:1 to 10:1 insert to plasmid. See paper methods for more details.
Proper citation: RRID:Addgene_73796 Copy
Species: Synthetic
Genetic Insert: Peredox
Vector Backbone Description: Vector Backbone:AAV-Flex; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26857245
Comments: Please note that this plasmid expresses only Peredox, with no mCherry or mCitrine to use for ratio measurements. It is best used for fluorescence lifetime imaging (FLIM).
Proper citation: RRID:Addgene_73807 Copy
Species: Synthetic
Genetic Insert: spa-GCaMP6s
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:3955; Vector Backbone:pGP-CMV (pEGFP-N1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26167640
Proper citation: RRID:Addgene_67559 Copy
Species: Synthetic
Genetic Insert: spa-GCaMP6m
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:3955; Vector Backbone:pGP-CMV (pEGFP-N1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26167640
Proper citation: RRID:Addgene_67557 Copy
Species: Synthetic
Genetic Insert: spa-GCaMP6f
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:3955; Vector Backbone:pGP-CMV (pEGFP-N1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26167640
Proper citation: RRID:Addgene_67558 Copy
Species: Synthetic
Genetic Insert: spa-GCaMP6s
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:3955; Vector Backbone:pGP-CMV (pEGFP-N1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26167640
Proper citation: RRID:Addgene_67556 Copy
Species: Synthetic
Genetic Insert: sspa-GCaMP6m
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:3955; Vector Backbone:pGP-CMV (pEGFP-N1); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26167640
Proper citation: RRID:Addgene_67560 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:2897; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26167640
Proper citation: RRID:Addgene_67564 Copy
Species: Synthetic
Genetic Insert: pa-GCaMP6f
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:2897; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26167640
Proper citation: RRID:Addgene_67570 Copy
Species: Synthetic
Genetic Insert: APEX2-GBP
Vector Backbone Description: Vector Backbone:pCSDEST2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26585296
Proper citation: RRID:Addgene_67651 Copy
Species: Synthetic
Genetic Insert: Hexahistidine/Maltose binding protein/MS2 coat protein fusion
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5422; Vector Backbone:pET30b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17548432
Comments: The MS2 coat protein in this plasmid contains the V29/dIFG mutations described in Lim and Peabody 1994 (Nucleic Acids Res. 1994 Sep 11;22(18):3748-52) which are reported to prevent coat protein multimerization and increase affinity for RNA.
Proper citation: RRID:Addgene_67717 Copy
Species: Synthetic
Genetic Insert: Transcription cassette for 3'-tagged RNAs
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17548432
Proper citation: RRID:Addgene_67715 Copy
Species: Synthetic
Genetic Insert: Transcription cassette for 3'-tagged RNAs
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17548432
Proper citation: RRID:Addgene_67716 Copy
Species: Synthetic
Genetic Insert: mKate2-P2A-APEX2-NS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR 221; Vector Types:Gateway multisite middle entry clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26585296
Proper citation: RRID:Addgene_67670 Copy
Species: Synthetic
Genetic Insert: APEX2-P2A-mKate2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR P2R-p3; Vector Types:Gateway multisite 3' entry clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26585296
Proper citation: RRID:Addgene_67671 Copy
Species: Synthetic
Genetic Insert: ML1N*2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR P2R-p3; Vector Types:Gateway multisite 3' entry clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26585296
Proper citation: RRID:Addgene_67678 Copy
Species: Synthetic
Genetic Insert: 2xFYVEhrs
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR P2R-p3; Vector Types:Gateway multisite 3' entry clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26585296
Proper citation: RRID:Addgene_67676 Copy
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