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Species: Ciona intestinalis
Genetic Insert: fluorescent protein voltage sensor
Vector Backbone Description: Backbone Size:5694; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22958819
Comments: http://www.fluorogenetic-voltage-sensors.org/
This construct is composed of the S1-4 domain of the CiVSP voltage sensor. Super ecliptic pHluorin is inserted at position A242 in the CiVSP sequence.
Proper citation: RRID:Addgene_36857 Copy
Species: Homo sapiens
Genetic Insert: SIRT6-TALEN_Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCCGGGAGGCCGCACTTG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36844 Copy
Vector Backbone Description: Backbone Size:7037; Vector Backbone:pCMMP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12972608
Comments: Sugden lab plasmid p3051
Proper citation: RRID:Addgene_36953 Copy
Genetic Insert: LMP1
Vector Backbone Description: Backbone Marker:Addgene plasmid #36953; Backbone Size:7037; Vector Backbone:pCMMP-MCS-IRES-eGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22318153
Comments: Sugden lab plasmid p3455
Please note that the full plasmid sequence provided by the depositing laboratory and Addgene's sequencing results differ from GenBank entry YP_401722.1 by three polymorphisms: F106Y, I122L and M129I. These differences result from the particular EBV isolate. For more information please see the following article: https://www.ncbi.nlm.nih.gov/pubmed/7931160
Proper citation: RRID:Addgene_36955 Copy
Species: Homo sapiens
Genetic Insert: EglN2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6886; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22343896
Comments: this plasmid was constructed in the lab of a HHMI-investigator
Proper citation: RRID:Addgene_36950 Copy
Species: Homo sapiens
Genetic Insert: EglN3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6887; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22343896
Comments: this plasmid was constructed in the lab of a HHMI-investigator
Proper citation: RRID:Addgene_36951 Copy
Vector Backbone Description: Backbone Size:7037; Vector Backbone:pCMMP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18042799
Comments: Sugden lab plasmid p3048
Proper citation: RRID:Addgene_36952 Copy
Species: Homo sapiens
Genetic Insert: DNMT3B2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15861382
Comments: The multiple cloning region of the pcDNA3 vector has been modified. In the modified vector, referred to as pcDNA3/Myc, the region of the multiple cloning site located between the HindIII and NotI sites was replaced by a DNA segment containing a Kozak consensus sequence, an ATG start site and the Myc epitope sequence followed by EcoRI restriction site (see supplemental document). The construction of pcDNA3/Myc has been described in Chen, Z.X., et al. J Cell Biochem, 95: 902-917.
The full-length cDNA for human DNMT3B2 was cloned into EcoRI and BamHI sites of pcDNA3/Myc. Constructed by Zhaoxia Chen.
Proper citation: RRID:Addgene_36942 Copy
Species: Aequorea victoria
Genetic Insert: Venus (EYFP)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6887; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22343896
Comments: this plasmid was constructed in the laboratory of an HHMI investigator
Proper citation: RRID:Addgene_36948 Copy
Species: Homo sapiens
Genetic Insert: CDKN2A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7800; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22249253
Proper citation: RRID:Addgene_37104 Copy
Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7800; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22249253
Comments: Also known as PSM.7LP (Knudsen and Wang 1997 MCB 17:5771).
Proper citation: RRID:Addgene_37106 Copy
Species: Homo sapiens
Genetic Insert: PHLPP1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21454620
Comments: Please note that the insert in this plasmid was referred to as PHLPP1beta in the original publication (Brognard et al., Mol. Cell 2007) and the plasmid was first described in Warfel et al. 2011.
Proper citation: RRID:Addgene_37100 Copy
Species: Homo sapiens
Genetic Insert: Human SMN1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16301532
Proper citation: RRID:Addgene_37057 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid #37060; Vector Backbone:pHSS6; Vector Types:; Bacterial Resistance:Kanamycin
Comments: Used to create strains to complement mutations using the NICS system (Mehr and Seifert, 1998; Mehr, 2000). Insert a functional copy of the gene ectopically at an unlinked chromosomal locus between the lctP and aspC genes under control of lac regulatory sequences, and linked to an Erythromycin-resistance cassette.
Proper citation: RRID:Addgene_37058 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid #1764; Backbone Size:4517; Vector Backbone:pBABE-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22371484
Proper citation: RRID:Addgene_36999 Copy
Species: Homo sapiens
Genetic Insert: Dsg2
Vector Backbone Description: Backbone Marker:K. Green lab; Vector Backbone:pmCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22184201
Proper citation: RRID:Addgene_36991 Copy
Species: Bovine Papillomavirus
Genetic Insert: BPV1 E6
Vector Backbone Description: Vector Backbone:N-Tap-Flag-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22547818
Proper citation: RRID:Addgene_37047 Copy
Species: Homo sapiens
Genetic Insert: Smad4
Vector Backbone Description: Backbone Marker:Weinberg Lab; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21245094
Comments: Insert was blunt-ended and ligated into SnaBI-digested pBabe-puro vector
Proper citation: RRID:Addgene_37041 Copy
Species: Mus musculus
Genetic Insert: slug
Vector Backbone Description: Backbone Marker:Dr. Tal Kafri; Backbone Size:0; Vector Backbone:pTK380 (tetracycline inducible); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: Slug cDNA was PCR amplified from an IMAGE clone (Open Biosystems) and cloned into BamHI-XbaI sites of pTK380.
Proper citation: RRID:Addgene_36986 Copy
Species: Homo sapiens
Genetic Insert: Dsg2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987342
Comments: The construct encoding human Dsg2 cDNA in pBluescript was a gift from Drs. W. Franke and S. Schafer, German Cancer Research Center, Heidelberg, Germany.
Proper citation: RRID:Addgene_36989 Copy
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