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pcDNA3/ TO myc-cCE-bio (cCE-bio)
 
Resource Report
Resource Website 		RRID:Addgene_82473 Myc-NES-mCE (Wt, NLS deletion)-TEV-Bio Mus musculus Ampicillin PMID:25137142 Backbone Size:5476; Vector Backbone:pcDNA3-T/O; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:20:58 0
pcDNA3-myc NES- mCE(K294A)
 
Resource Report
Resource Website 		RRID:Addgene_82464 NES mRNA capping enzyme Mus musculus Ampicillin PMID:19223470 Backbone Size:7224; Vector Backbone:pcDNA3-myc-mCE(K294A); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin K294A 2026-08-15 01:20:58 0
pcDNA3-myc NES-mCE NLS
 
Resource Report
Resource Website 		RRID:Addgene_82466 NES mRNA capping enzyme Mus musculus Ampicillin PMID:19223470 Backbone Size:5468; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:20:56 0
pcDNA3-myc NES-mCE
 
Resource Report
Resource Website 		RRID:Addgene_82463 NES mRNA capping enzyme Mus musculus Ampicillin PMID:19223470 Backbone Size:7224; Vector Backbone:pcDNA3-myc-mCE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:20:56 0
pTRIPZ (M)-HT-Cbx4
 
Resource Report
Resource Website 		RRID:Addgene_82513 Chromobox Homolog 4 Mus musculus Ampicillin Backbone Size:12300; Vector Backbone:pTRIPZ (M); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:20:57 0
p204_LTJ_sgRNACD45.2_R2
 
Resource Report
Resource Website 		RRID:Addgene_82673 sgRNA targeting CD45.2 region 2 Mus musculus Ampicillin PMID:29445007 Plasmid co-expresses Cas9 from S. pyogenes with 2A-EGFP, as part of the pX458 backbone. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544 Backbone Marker:Feng Zhang (Addgene plasmid# 48138); Backbone Size:9300; Vector Backbone:pX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:21:00 0
p236_LTJ_sgRNAFoxp3sf/J
 
Resource Report
Resource Website 		RRID:Addgene_82676 sgRNA targeting Foxp3 sf/J Mus musculus Ampicillin PMID:29445007 Plasmid co-expresses Cas9 from S. pyogenes with 2A-EGFP, as part of the pX458 backbone. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544 Backbone Marker:Feng Zhang (Addgene plasmid# 48138); Backbone Size:9300; Vector Backbone:pX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:21:00 0
p163_LTJ_sgRNACD90.1
 
Resource Report
Resource Website 		RRID:Addgene_82677 sgRNA targeting mouse CD90.1 Mus musculus Ampicillin PMID:29445007 Plasmid co-expresses Cas9 from S. pyogenes with 2A-EGFP, as part of the pX458 backbone. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544 Backbone Marker:Feng Zhang (Addgene plasmid# 48138); Backbone Size:9300; Vector Backbone:pX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:20:58 0
p184_LTJ_sgRNACD90.2
 
Resource Report
Resource Website 		RRID:Addgene_82670 sgRNA targeting mouse CD90.2 Mus musculus Ampicillin PMID:29445007 Plasmid co-expresses Cas9 from S. pyogenes with 2A-EGFP, as part of the pX458 backbone. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544 Backbone Marker:Feng Zhang (Addgene plasmid# 48138); Backbone Size:9300; Vector Backbone:pX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:21:00 0
p202_LTJ_sgRNACD45.2_R1
 
Resource Report
Resource Website 		RRID:Addgene_82672 sgRNA targeting mouse CD45.2 region 1 Mus musculus Ampicillin PMID:29445007 Plasmid co-expresses Cas9 from S. pyogenes with 2A-EGFP, as part of the pX458 backbone. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544 Backbone Marker:Feng Zhang (Addgene plasmid# 48138); Backbone Size:9300; Vector Backbone:pX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:20:58 0
p249_LTJ_1kbFoxp3wtTemplate
 
Resource Report
Resource Website 		RRID:Addgene_82664 1kb genomic DNA sequence from murine wildtype Foxp3 Mus musculus Ampicillin PMID:29445007 This HDR template can be used in conjunction with sgRNA Foxp3K276X (Addgene ID 82675) or sgRNA Foxp3sf/J (Addgene ID 82676). In the template we introduced a PAM mutation. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544 Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:HDR template for gene editing; Bacterial Resistance:Ampicillin 2026-08-15 01:21:00 0
p250_LTJ_2kbFoxp3wtTemplate
 
Resource Report
Resource Website 		RRID:Addgene_82665 2kb genomic DNA sequence from murine wildtype Foxp3 Mus musculus Ampicillin PMID:29445007 This HDR template can be used in conjunction with sgRNA Foxp3K276X (Addgene ID 82675) or sgRNA Foxp3sf/J (Addgene ID 82676). In the template we introduced a PAM mutation. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544 Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:HDR template for gene editing; Bacterial Resistance:Ampicillin 2026-08-15 01:20:58 0
p213_LTJ_160bpCD90.1Template
 
Resource Report
Resource Website 		RRID:Addgene_82666 160bp genomic DNA sequence from murine CD90.1 Mus musculus Ampicillin PMID:29445007 This HDR template can be used in conjunction with sgRNA CD90.2 (Addgene ID 82670 or Addgene ID 82671). In the template we introduced a PAM mutation. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544 Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:HDR template for gene editing; Bacterial Resistance:Ampicillin 2026-08-15 01:20:58 0
pmNFL
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_83127 mouse NFL Mus musculus Kanamycin PMID:17285620 Plasmid first described in Yan et al. (2007), "The polypeptide composition of moving and stationary neurofilaments in cultured sympathetic neurons", Cell Motility and the Cytoskeleton 64:299–309. Mouse NFL cDNA sequence deposited in Genbank (Accession number DQ201635). Backbone Marker:Clontech; Backbone Size:4242; Vector Backbone:pEGFP-C1 with EGFP sequence removed; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:21:05 2
pmNFM
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_83126 mouse NFM Mus musculus Kanamycin PMID:17285620 Plasmid first described in Yan et al. (2007), "The polypeptide composition of moving and stationary neurofilaments in cultured sympathetic neurons", Cell Motility and the Cytoskeleton 64:299–309. Mouse NFM cDNA sequence deposited in Genbank (Accession number DQ201636). Backbone Marker:Clontech; Backbone Size:4047; Vector Backbone:pEGFP-C1 with EGFP sequence removed; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:21:02 1
TET-pLKO.1 PURO shId2 #1
 
Resource Report
Resource Website 		RRID:Addgene_83088 shId2 Mus musculus Ampicillin PMID:29161592 The targeting sequence was identified from the TRC ( TRCN0000229535). Knockdown validated by Western blot of transient, ectopic expression of murine Id2 in 293T cells. Backbone Marker:Addgene #21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:21:02 0
pSico-shNKCC1
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_83027 shNKCC1 Mus musculus Ampicillin PMID:23015452 This plasmid contains a c4351t substitution within the shRNA. The depositing lab states that they do not know whether this mutation affects plasmid function. pSico allows for conditional (Cre-Lox), stable expression of shNKCC1. Cre expression excises GFP and allows for shNKCC1 expression. Backbone Marker:Jacks Lab (MIT); Backbone Size:7566; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-15 01:21:01 1
CAPZB2-TwinStrepII
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_83194 CAPZB2 Mus musculus Ampicillin Backbone Marker:Vector Builder ; Backbone Size:3855; Vector Backbone:pRP[Exp]-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:21:03 1
CAPZA1-TwinStrepII
 
Resource Report
Resource Website 		RRID:Addgene_83193 CAPZA1 Mus musculus Ampicillin Backbone Marker:Vector Builder ; Backbone Size:3855; Vector Backbone:pRP[Exp]-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:21:03 0
TMC1-TwinStrepII
 
Resource Report
Resource Website 		RRID:Addgene_83267 TMC1 Mus musculus Ampicillin Backbone Marker:Vector Builder ; Backbone Size:3855; Vector Backbone:pRP[Exp]-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:21:03 0

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