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Species: Saccharomyces cerevisiae
Genetic Insert: CAN1-LUC
Vector Backbone Description: Vector Backbone:pSP65A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18496529
Comments: Note: the poly A tends to undergo recombination to a shorter tract. It is important to sequence a few isolates from a transformation or prep to ensure integrity. Please see Fig 1 of associated publication for general schematic
Proper citation: RRID:Addgene_78484 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI
Vector Backbone Description: Vector Backbone:pColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35007422
Proper citation: RRID:Addgene_179578 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: β′-COPI-WD40
Vector Backbone Description: Backbone Size:5731; Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35136165
Proper citation: RRID:Addgene_180753 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-APEX2
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, TEM imaging; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182211 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Backbone Marker:Miller et al (1998) Nucleic Acids Res 26(15):3577-3583 ; Backbone Size:6512; Vector Backbone:pBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_40612 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Backbone Marker:Miller et al (1998) Nucleic Acids Res 26(15):3577-3583 ; Backbone Size:6512; Vector Backbone:pBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_40611 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LifeAct
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmcherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22328533
Proper citation: RRID:Addgene_40908 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1 1-153
Vector Backbone Description: Backbone Size:6267; Vector Backbone:pRS416 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18480252
Proper citation: RRID:Addgene_18690 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1
Vector Backbone Description: Backbone Size:5574; Vector Backbone:pRS416 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18480252
Proper citation: RRID:Addgene_18684 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1
Vector Backbone Description: Backbone Size:7099; Vector Backbone:pRS426 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18480252
Proper citation: RRID:Addgene_18686 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sis1
Vector Backbone Description: Backbone Size:5665; Vector Backbone:pRS414 GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18480252
Proper citation: RRID:Addgene_18687 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sis1 deltaG/F
Vector Backbone Description: Backbone Size:5665; Vector Backbone:pRS414 GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18480252
Proper citation: RRID:Addgene_18688 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ty5-HIS3AI
Vector Backbone Description: Backbone Size:4887; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18802438
Proper citation: RRID:Addgene_18796 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1 L94A
Vector Backbone Description: Backbone Size:5578; Vector Backbone:pRS416 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18480252
Proper citation: RRID:Addgene_18691 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ste7
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pYBS317; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8062390
Comments: Has a 0.5kb BamHI-BglII fragmetn from pYEE129 at BamHI of pYBS317. The open reading frame reads through to an unknown stop in the vector.
Proper citation: RRID:Addgene_18885 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ste11
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pYBS317; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8062390
Comments: Lex-Ste11 has the 0.9kb PflMI-SalI fragment of pYBS345 (the PCR-derived Ste11 in pYBS317; see article) deleted by blunt-end ligation with Klenow. The open reading frame reads through to an unknown stop in the vector.
Proper citation: RRID:Addgene_18887 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FAR1
Vector Backbone Description: Backbone Size:6449; Vector Backbone:pJG4-5; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8062390
Comments: A vector like pJG4-5 with the BamHI site in reading frame CCA TGG by inserting EcoRI-SalI of the pUC19 polylinker
Proper citation: RRID:Addgene_18889 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FUS3
Vector Backbone Description: Backbone Size:10100; Vector Backbone:Lex202pl; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2406028
Proper citation: RRID:Addgene_18899 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FAR1
Vector Backbone Description: Backbone Size:6449; Vector Backbone:pJG4-5; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8314085
Comments: pYBS139 is a derivative of pYBS135 in which the EcoRI site was filled n to create the proper reading frame.
Proper citation: RRID:Addgene_18900 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YPT52
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18843 Copy
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