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Genetic Insert: rAPOBEC1-pcoCas9D10A-UGI
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30928637
Proper citation: RRID:Addgene_124310 Copy
Genetic Insert: pcoCas9D10A-PmCDA1-UGI
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30928637
Proper citation: RRID:Addgene_124312 Copy
Species: Homo sapiens
Genetic Insert: Halo tag
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30358421
Proper citation: RRID:Addgene_124316 Copy
Species: Homo sapiens
Genetic Insert: AML1-ETO fusion
Vector Backbone Description: Backbone Marker:See comments; Vector Backbone:MigR1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15569932
Comments: MigR1 is a MSCV-IRES-EGFP retroviral construct, similar to pMig (Addgene vector 9044). See "Pear, Baltimore, Blood. 1998 Nov 15;92(10):3780-92".
Proper citation: RRID:Addgene_12431 Copy
Species: Mus musculus
Genetic Insert: shP53
Vector Backbone Description: Backbone Size:6491; Vector Backbone:pT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30760578
Proper citation: RRID:Addgene_124261 Copy
Species: Homo sapiens
Genetic Insert: TMEM173
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124264 Copy
Species: Homo sapiens
Genetic Insert: LMNA
Vector Backbone Description: Vector Backbone:mRFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_124268 Copy
Species: Homo sapiens
Genetic Insert: ZsGreen
Vector Backbone Description: Vector Backbone:pLCE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31300418
Proper citation: RRID:Addgene_124301 Copy
Species: Homo sapiens
Genetic Insert: CRBN
Vector Backbone Description: Vector Backbone:pLCE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31300418
Proper citation: RRID:Addgene_124303 Copy
Species: Homo sapiens
Genetic Insert: Isocitrate dehydrogenase 1 (R132H)
Vector Backbone Description: Backbone Size:4891; Vector Backbone:pKT; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30760578
Proper citation: RRID:Addgene_124257 Copy
Species: Mus musculus
Genetic Insert: shATRX
Vector Backbone Description: Backbone Size:7261; Vector Backbone:pT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30760578
Proper citation: RRID:Addgene_124259 Copy
Species: Mus musculus
Genetic Insert: ISG15
Vector Backbone Description: Backbone Size:4790; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15485925
Proper citation: RRID:Addgene_12445 Copy
Species: Mus musculus
Genetic Insert: C-terminal processed form of ISG15
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16275353
Proper citation: RRID:Addgene_12448 Copy
Species: Homo sapiens
Genetic Insert: UbcH8
Vector Backbone Description: Backbone Size:4679; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15485925
Proper citation: RRID:Addgene_12442 Copy
Species: Synthetic
Genetic Insert: FlipCherry-T2A-GFP
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30821975
Proper citation: RRID:Addgene_124436 Copy
Vector Backbone Description: Vector Backbone:pCOLA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31185158
Comments: GenBank file with original annotations can be downloaded from the "Resource Information" section. Please visit https://www.biorxiv.org/content/10.1101/611574v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_124422 Copy
Species: Synthetic
Genetic Insert: FlipGFP(Casp3 cleavage seq) T2A mCherry
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30821975
Proper citation: RRID:Addgene_124428 Copy
Genetic Insert: Tornado-F30-Broccoli-aptNFkB#5
Vector Backbone Description: Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30962542
Proper citation: RRID:Addgene_124362 Copy
Species: Synthetic
Genetic Insert: SpyDock
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3641; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30988307
Proper citation: RRID:Addgene_124618 Copy
Genetic Insert: TCF/LEF binding sites
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4871; Vector Backbone:pTA-Luc; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: This is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold (activation by phosphorylation mutants of beta-catenin). The appropriate control plasmid is clone M51, Super8XFOPflash, which has mutant TCF/LEF binding sites.
This construct was made by Ajamete Kaykas in the Moon lab. The backbone is the pTA-Luc vector of Clontech, which provides a minimal TA viral promoter driving expression of the firefly luciferase gene (see company publications for details). 7 TCF/LEF binding sites were cloned into the Mlu1 site of this vector (7 copies of: AGATCAAAGGgggta, with TCF/LEF binding site in CAP letters, and a spacer in lower case, separating each copy of the TCF/LEF site).
Note: This plasmid was published as M50 Super 8x TOPFlash, but the plasmid actually contains 7 TCF/LEF sites.
Proper citation: RRID:Addgene_12456 Copy
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