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Species: Saccharomyces cerevisiae
Genetic Insert: pCTT1 24xPP7sl
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Comments: Addgene's lab has found that this plasmid encodes 22 PP7 stem loops rather than the reported 24. Depositor noted that PP7 stem loop repeat region is prone to losing repeats over time and recommends verifying repeats by whole plasmid sequencing to avoid further loss of repeats.
Proper citation: RRID:Addgene_139488 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pALD3 24xPP7sl
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Comments: Addgene's lab has found that this plasmid encodes 22 PP7 stem loops rather than the reported 24. Depositor noted that PP7 stem loop repeat region is prone to losing repeats over time and recommends verifying repeats by whole plasmid sequencing to avoid further loss of repeats.
Proper citation: RRID:Addgene_139492 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pSTL1 24xMS2sl
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Comments: Depositor noted that MS2 stem loop repeat region is prone to losing repeats over time and recommends verifying repeats by whole plasmid sequencing.
Proper citation: RRID:Addgene_139493 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pADH1 PP7∆FG-mCherry tCYC1
Vector Backbone Description: Backbone Size:3861; Vector Backbone:pSIVURA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Proper citation: RRID:Addgene_139496 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pADH1 MS2-GFPenvy tCYC1
Vector Backbone Description: Backbone Size:3861; Vector Backbone:pSIVURA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Proper citation: RRID:Addgene_139497 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pGPD Cas9 / sgRNA (STL162)
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS423II; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Proper citation: RRID:Addgene_139498 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pSTL1 24xPP7sl STL1 100 - 600
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32576833
Proper citation: RRID:Addgene_139499 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ABKAR(T/A)
Vector Backbone Description: Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25788287
Proper citation: RRID:Addgene_140052 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ABKAR
Vector Backbone Description: Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25788287
Proper citation: RRID:Addgene_140051 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MUP1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32584255
Comments: Parental strain JK93da.
Proper citation: RRID:Addgene_140152 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NDT1 with 5' and 3' UTRs
Vector Backbone Description: Vector Backbone:YCplac33C; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33087354
Proper citation: RRID:Addgene_140593 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 2pabPAC
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33074647
Proper citation: RRID:Addgene_158968 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yHS1-M7A
Vector Backbone Description: Backbone Marker:M. Funk; Backbone Size:6900; Vector Backbone:p415GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27247412
Comments: The first-generation heme sensor, HS1, consists of a heme-binding domain, the His/Met coordinating 4-alpha-helical bundle hemoprotein cytochrome b562 (Cyt b562) (13), fused to a pair of fluorescent proteins, EGFP and Katushka 2 (mKATE2)
Proper citation: RRID:Addgene_159151 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yHS1-M7A,H102A
Vector Backbone Description: Backbone Marker:M. Funk; Backbone Size:6900; Vector Backbone:p415GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27247412
Comments: The first-generation heme sensor, HS1, consists of a heme-binding domain, the His/Met coordinating 4-alpha-helical bundle hemoprotein cytochrome b562 (Cyt b562) (13), fused to a pair of fluorescent proteins, EGFP and Katushka 2 (mKATE2)
Proper citation: RRID:Addgene_159152 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: nuc-yHS1-M7A,H102A
Vector Backbone Description: Backbone Marker:M. Funk; Backbone Size:6900; Vector Backbone:p415GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27247412
Comments: The first-generation heme sensor, HS1, consists of a heme-binding domain, the His/Met coordinating 4-alpha-helical bundle hemoprotein cytochrome b562 (Cyt b562) (13), fused to a pair of fluorescent proteins, EGFP and Katushka 2 (mKATE2)
Proper citation: RRID:Addgene_159168 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: nuc-yHS1
Vector Backbone Description: Backbone Marker:M. Funk; Backbone Size:6900; Vector Backbone:p415GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27247412
Comments: The first-generation heme sensor, HS1, consists of a heme-binding domain, the His/Met coordinating 4-alpha-helical bundle hemoprotein cytochrome b562 (Cyt b562) (13), fused to a pair of fluorescent proteins, EGFP and Katushka 2 (mKATE2)
Proper citation: RRID:Addgene_159166 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET3 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:A. Geoghegan (Rockefeller University); Backbone Size:5578; Vector Backbone:p405MET3b; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_15962 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET25 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:3814; Vector Backbone:pRS405; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).
Proper citation: RRID:Addgene_15961 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ufd4
Vector Backbone Description: Backbone Size:6707; Vector Backbone:pRS415-Gal1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159590 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TEF1 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:N. Buchler; Backbone Size:5074; Vector Backbone:p405GALS; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_15968 Copy
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