Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Synthetic
Genetic Insert: II-AAAC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43220 Copy
Species: Synthetic
Genetic Insert: II-CGTT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43330 Copy
Species: Synthetic
Genetic Insert: II-CTCA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43335 Copy
Species: Synthetic
Genetic Insert: III-CACC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43544 Copy
Genetic Insert: None
Vector Backbone Description: Backbone Marker:Joung lab MLM3636; Vector Backbone:Human-gRNA-ExpressionVector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Human cell gRNA Expression Vector for use with JDS246 (Addgene plasmid# 43861--mammalian codon-optimized streptococcus pyogenes Cas9). See the supplemental document "Joung Lab gRNA Cloning Protocol" for more details.
Proper citation: RRID:Addgene_43860 Copy
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43859 Copy
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22245580
Comments: NOTE: there is a second, non-annotated EcoRI site present in this plasmid. The function of the plasmid will not be impaired; however,
you cannot use the EcoRI site in the MCS for cloning.
Proper citation: RRID:Addgene_43796 Copy
Species: Homo sapiens
Genetic Insert: LIN28B
Vector Backbone Description: Backbone Size:5372; Vector Backbone:pFRT/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23481595
Comments: Note that the Flag and HA tags are not in frame in the depositor's assembled sequence, but Addgene's quality control sequence shows they are in frame.
Proper citation: RRID:Addgene_43798 Copy
Vector Backbone Description: Backbone Marker:John Mekalanos lab, Harvard Medical School (PMID: 2836362); Backbone Size:5720; Vector Backbone:pGP704; Vector Types:Bacterial Expression, Bacterial allelic exchange vector with sacB1; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9511756
Comments: The plasmids deposited here comprise a set of SacB1-dependent allelic exchange vectors improved from a previously described suicide vector, pGP704 (Miller and Mekalanos, 1988), by including a system to select for plasmid loss by recombination.
Plasmid pRE107 was constructed by cloning the appropriate EcoRI fragment from pUC58-sacB1 (McIver et al., 1995) into pGP704 (see associated schematic image). The sacB1 allele is a modified variation of sacB, where unique restriction sites were removed by site directed mutagenesis (McIver et al., 1995). Additionally, the BamHI site in the R6K ori of the resulting plasmid was removed by partial BamHI digestion, blunting the resulting overhangs with PolIk (resulting in the formation of a ClaI site) and screening for its loss by restriction analysis.
The resulting plasmid together with others in this deposited series contain the conditional R6K ori, the origin of transfer (oriT) which allows conjugative transfer from permissive hosts, the sacB1 gene to provide negative selection and a MCS, along with a range of different AbR markers.
Note: Addgene's NGS results found that a large portion of the plasmid backbone (containing the oriT feature) is in the opposite orientation compared to the depositor's reference sequence.
Proper citation: RRID:Addgene_43829 Copy
Species: Homo sapiens
Genetic Insert: human WNT11
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43824 Copy
Species: Homo sapiens
Genetic Insert: WNT8A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43818 Copy
Species: Homo sapiens
Genetic Insert: WNT8B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43819 Copy
Species: Homo sapiens
Genetic Insert: WNT4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43812 Copy
Species: Homo sapiens
Genetic Insert: WNT5A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43813 Copy
Species: Homo sapiens
Genetic Insert: human WNT7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43816 Copy
Species: Homo sapiens
Genetic Insert: WNT2B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43808 Copy
Species: Homo sapiens
Genetic Insert: Human Optimized S. pyogenes Cas9
Vector Backbone Description: Backbone Size:6670; Vector Backbone:p415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23460208
Proper citation: RRID:Addgene_43804 Copy
Species: Mus musculus
Genetic Insert: Hes1 Promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5597; Vector Backbone:pGL2-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7906273
Proper citation: RRID:Addgene_43806 Copy
Genetic Insert: SID4X Domain
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22828628
Comments: This enhanced SID domain (SID4X domain) is to be used with TALE effectors to create TALE repressors, as described in Cong et al. Nature Communications. This new domain replaces the previous SID domain reported in the original article.
Proper citation: RRID:Addgene_43882 Copy
Species: Synthetic
Genetic Insert: GCaMP6s
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8071; Vector Backbone:pLVX-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31346185
Proper citation: RRID:Addgene_164589 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within RRID that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
