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Species: Homo sapiens
Genetic Insert: 14-3-3 sigma
Vector Backbone Description: Backbone Size:4679; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16352599
Proper citation: RRID:Addgene_12453 Copy
Species: Homo sapiens
Genetic Insert: EFP
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16352599
Comments: Digestion by KpnI and XhoI should give a 5.4 kb fragment and a 2.4 kb fragment.
Compared to GenBank ID NP_005073.2, the EFP insert in this plasmid appears to contain a P385L mutation, whereas when compared to GenBank ID AB384815.1 the EFP insert appears to contain G89V and A185T mutations. These apparent mutations may represent valid polymorphisms and reflect the sequence of the original human cDNA clone. The plasmid is exactly as described in the associated publication.
Proper citation: RRID:Addgene_12452 Copy
Species: Mus musculus
Genetic Insert: UBP43
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16710296
Proper citation: RRID:Addgene_12454 Copy
Species: Mus musculus
Genetic Insert: Soma-localized ChrimsonR
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:3000; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842660
Proper citation: RRID:Addgene_124603 Copy
Species: E. coli
Genetic Insert: Protein A and hyperactive Tn5 transposase (Tnp) fusion protein
Vector Backbone Description: Backbone Size:8079; Vector Backbone:pTXB1-Tn5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31036827
Comments: Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results to compare sequences.
This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but can reduce transformation or transfection efficiencies. If you have trouble isolating the monomeric version of this plasmid, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_124601 Copy
Species: Mus musculus
Genetic Insert: C1V1
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:4000; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842660
Proper citation: RRID:Addgene_124650 Copy
Species: Homo sapiens
Genetic Insert: c-Myc Renilla reporter
Vector Backbone Description: Backbone Marker:Venken Lab, #124525; Backbone Size:2326; Vector Backbone:AlphaC Vector; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32539183
Comments: Insert can be released with BsmBI for further cloning into Omega level vectors.
Proper citation: RRID:Addgene_124532 Copy
Species: Homo sapiens
Genetic Insert: RGECO-TnT
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:7500; Vector Backbone:pShuttle CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30732532
Proper citation: RRID:Addgene_124643 Copy
Genetic Insert: TurboID
Vector Backbone Description: Vector Backbone:pCD-betaG-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: A TurboID cDNA was amplified by PCR from V5-TurboID-NES_pcDNA3 (Plamid #107169).
Proper citation: RRID:Addgene_124646 Copy
Species: Homo sapiens
Genetic Insert: Ubc13
Vector Backbone Description: Backbone Size:0; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16122702
Comments: EcoRV site was destroyed during cloning and replaced with a new NotI site.
Proper citation: RRID:Addgene_12461 Copy
Species: Homo sapiens
Genetic Insert: Ubc13
Vector Backbone Description: Backbone Size:4679; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16122702
Proper citation: RRID:Addgene_12460 Copy
Species: Synthetic
Genetic Insert: CellTagA
Vector Backbone Description: Backbone Size:8426; Vector Backbone:pSMAL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31072405
Comments: The pSMAL backbone was a kind gift from John Dick: van Galen, P. et al. The unfolded protein response governs integrity of the haematopoietic stem-cell pool during stress. Nature 510, 268–72 (2014).
Please visit https://www.biorxiv.org/content/10.1101/335547v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_124591 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:p416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31885198
Comments: Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124583 Copy
Species: Synthetic
Genetic Insert: CggR promoter element
Vector Backbone Description: Vector Backbone:p416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31885198
Comments: Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124582 Copy
Species: Synthetic
Genetic Insert: cggR_R250A
Vector Backbone Description: Vector Backbone:pHO - plasmid; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31885198
Comments: Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124585 Copy
Species: Synthetic
Genetic Insert: cggR
Vector Backbone Description: Vector Backbone:pHO - plasmid; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31885198
Comments: Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124584 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590189
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. mTagBFP coding sequence was cloned from Addgene ID: 62383.
Proper citation: RRID:Addgene_105772 Copy
Species: Synthetic
Genetic Insert: stGtACR2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5360; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30297821
Proper citation: RRID:Addgene_105669 Copy
Species: Homo sapiens
Genetic Insert: human carbonic anhydrase II thermostable variant
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105667 Copy
Species: Homo sapiens
Genetic Insert: alpha-synuclein E46K
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25657004
Proper citation: RRID:Addgene_105730 Copy
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