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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pFlagCMV2-14-3-3sigma
 
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RRID:Addgene_12453 14-3-3 sigma Homo sapiens Ampicillin PMID:16352599 Backbone Size:4679; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:07 1
pcDNA3.0-HA-EFP
 
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RRID:Addgene_12452 EFP Homo sapiens Ampicillin PMID:16352599 Digestion by KpnI and XhoI should give a 5.4 kb fragment and a 2.4 kb fragment. Compared to GenBank ID NP_005073.2, the EFP insert in this plasmid appears to contain a P385L mutation, whereas when compared to GenBank ID AB384815.1 the EFP insert appears to contain G89V and A185T mutations. These apparent mutations may represent valid polymorphisms and reflect the sequence of the original human cDNA clone. The plasmid is exactly as described in the associated publication. Backbone Size:5400; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:07 5
pcDNA3.1-HA-mUbp43
 
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RRID:Addgene_12454 UBP43 Mus musculus Ampicillin PMID:16710296 Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:07 3
pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40
 
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RRID:Addgene_124603 Soma-localized ChrimsonR Mus musculus Ampicillin PMID:30842660 Backbone Marker:Genscript; Backbone Size:3000; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:04:07 4
3XFlag-pA-Tn5-Fl
 
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10+ mentions
RRID:Addgene_124601 Protein A and hyperactive Tn5 transposase (Tnp) fusion protein E. coli Ampicillin PMID:31036827 Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results to compare sequences. This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but can reduce transformation or transfection efficiencies. If you have trouble isolating the monomeric version of this plasmid, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid. Backbone Size:8079; Vector Backbone:pTXB1-Tn5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:08 32
pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1
 
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RRID:Addgene_124650 C1V1 Mus musculus Ampicillin PMID:30842660 Backbone Marker:Genscript; Backbone Size:4000; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:04:08 2
7xE-Box::Renilla
 
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1+ mentions
RRID:Addgene_124532 c-Myc Renilla reporter Homo sapiens Kanamycin PMID:32539183 Insert can be released with BsmBI for further cloning into Omega level vectors. Backbone Marker:Venken Lab, #124525; Backbone Size:2326; Vector Backbone:AlphaC Vector; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:04:07 2
pShuttle CMV RGECO-TnT
 
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RRID:Addgene_124643 RGECO-TnT Homo sapiens Kanamycin PMID:30732532 Backbone Marker:Agilent; Backbone Size:7500; Vector Backbone:pShuttle CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:04:08 1
Flag-TurboID
 
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1+ mentions
RRID:Addgene_124646 TurboID Ampicillin A TurboID cDNA was amplified by PCR from V5-TurboID-NES_pcDNA3 (Plamid #107169). Vector Backbone:pCD-betaG-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:08 7
pcDNA3.0-HA-UbcH13
 
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1+ mentions
RRID:Addgene_12461 Ubc13 Homo sapiens Ampicillin PMID:16122702 EcoRV site was destroyed during cloning and replaced with a new NotI site. Backbone Size:0; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:08 2
pFlagCMV2-UbcH13
 
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RRID:Addgene_12460 Ubc13 Homo sapiens Ampicillin PMID:16122702 Backbone Size:4679; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:07 2
CellTagA
 
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RRID:Addgene_124591 CellTagA Synthetic Ampicillin PMID:31072405 The pSMAL backbone was a kind gift from John Dick: van Galen, P. et al. The unfolded protein response governs integrity of the haematopoietic stem-cell pool during stress. Nature 510, 268–72 (2014). Please visit https://www.biorxiv.org/content/10.1101/335547v2 for bioRxiv preprint. Backbone Size:8426; Vector Backbone:pSMAL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:04:08 3
pTEF6-7
 
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1+ mentions
RRID:Addgene_124583 mCherry Ampicillin PMID:31885198 Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint Vector Backbone:p416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:07 1
pCggRO-reporter
 
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RRID:Addgene_124582 CggR promoter element Synthetic Ampicillin PMID:31885198 Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint Vector Backbone:p416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:07 2
pHO_pTEFmut7_CggR_R250A_ble
 
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RRID:Addgene_124585 cggR_R250A Synthetic Ampicillin PMID:31885198 Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint Vector Backbone:pHO - plasmid; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin changed arginine 250 to alanine 2026-08-15 01:04:07 3
pHO_pTEFmut7_CggR_ble
 
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RRID:Addgene_124584 cggR Synthetic Ampicillin PMID:31885198 Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint Vector Backbone:pHO - plasmid; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:04:08 1
pKK-mTagBFP-TEV
 
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RRID:Addgene_105772 Ampicillin PMID:29590189 This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. mTagBFP coding sequence was cloned from Addgene ID: 62383. Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin 2026-08-15 01:00:55 1
pAAV-CKIIa-stGtACR2-FusionRed
 
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10+ mentions
RRID:Addgene_105669 stGtACR2 Synthetic Ampicillin PMID:30297821 Backbone Marker:Invitrogen; Backbone Size:5360; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:00:54 19
pBad-CA TAG93
 
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RRID:Addgene_105667 human carbonic anhydrase II thermostable variant Homo sapiens Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin TAG stop codon at site 93 2026-08-15 01:00:54 1
pcDNA6 asyn E46K
 
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RRID:Addgene_105730 alpha-synuclein E46K Homo sapiens Ampicillin PMID:25657004 Backbone Marker:Invitrogen; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin E46K 2026-08-15 01:00:54 1

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