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Species: Synthetic
Genetic Insert: SpsCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68319 Copy
Species: Synthetic
Genetic Insert: Ste7DS NLS(4A) mCherry
Vector Backbone Description: Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26231587
Proper citation: RRID:Addgene_68275 Copy
Species: Synthetic
Genetic Insert: Ste7DS NLS mCherry
Vector Backbone Description: Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26231587
Proper citation: RRID:Addgene_68274 Copy
Species: Synthetic
Genetic Insert: CdCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68314 Copy
Species: Synthetic
Genetic Insert: Ste7DS NLS mCitrine
Vector Backbone Description: Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26231587
Proper citation: RRID:Addgene_68278 Copy
Species: Synthetic
Genetic Insert: AzoCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68329 Copy
Species: Synthetic
Genetic Insert: SepRS9/EF-Sep21, 1xtRNA-Sep
Vector Backbone Description: Vector Backbone:pKD; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26350500
Comments: See supplementary information accompanying article for additional cloning information. We recommend introducing this plasmid into a recoded strain of E. coli (no genomic TAG codons) for high-efficiency phoshoprotein expression.
Proper citation: RRID:Addgene_68284 Copy
Species: Synthetic
Genetic Insert: FtCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68326 Copy
Species: Synthetic
Genetic Insert: BcCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68325 Copy
Species: Synthetic
Genetic Insert: LfCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68323 Copy
Species: Synthetic
Genetic Insert: SepRS/EF-Sep, 2xtRNA-Sep-A37
Vector Backbone Description: Vector Backbone:pKD; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26350500
Comments: See supplementary information accompanying article for additional cloning information. We recommend introducing this plasmid into a recoded strain of E. coli (no genomic TAG codons) for high-efficiency phoshoprotein expression.
Proper citation: RRID:Addgene_68289 Copy
Species: Synthetic
Genetic Insert: SpaCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68322 Copy
Species: Synthetic
Genetic Insert: SepRS9/EF-Sep21, 2xtRNA-Sep
Vector Backbone Description: Vector Backbone:pKD; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26350500
Comments: See supplementary information accompanying article for additional cloning information. We recommend introducing this plasmid into a recoded strain of E. coli (no genomic TAG codons) for high-efficiency phoshoprotein expression.
Proper citation: RRID:Addgene_68288 Copy
Species: Synthetic
Genetic Insert: GFP Q157TAG
Vector Backbone Description: Vector Backbone:pCRT7/NT-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26350500
Comments: Encodes reporter protein to assess efficiency of phosphoserine incorporation at TAG codons.
Proper citation: RRID:Addgene_68297 Copy
Species: Synthetic
Genetic Insert: GdCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68330 Copy
Species: Synthetic
Genetic Insert: GFP S2TAG
Vector Backbone Description: Vector Backbone:pCRT7/NT-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26350500
Comments: Encodes reporter protein to assess efficiency of phosphoserine incorporation at TAG codons.
Proper citation: RRID:Addgene_68296 Copy
Species: Synthetic
Genetic Insert: CjCas9
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68338 Copy
Species: Synthetic
Genetic Insert: St3Cas9
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68337 Copy
Species: Synthetic
Genetic Insert: ClCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68336 Copy
Species: Synthetic
Genetic Insert: PlCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68333 Copy
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