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Species: Saccharomyces cerevisiae
Genetic Insert: eRF3
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34413231
Proper citation: RRID:Addgene_175420 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eRF1
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34413231
Proper citation: RRID:Addgene_175414 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eRF1
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34413231
Proper citation: RRID:Addgene_175415 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eRF3
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34413231
Proper citation: RRID:Addgene_175419 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eRF1
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34413231
Proper citation: RRID:Addgene_175413 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Backbone Size:8902; Vector Backbone:pBDP; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18621688
Comments: pBPGw is a modular Gateway compatible promoter-less GAL4 vector amenable to high throughput in vitro cloning using Invitrogen LR clonase and specific in vivo genomic targeting using PhiC31 integrase. In addition the GAL4 CDS and yeast transcriptional terminator can easily be substituted for another driver by a directional 5' KpnI to 3' HindIII digest.
Proper citation: RRID:Addgene_17574 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Backbone Size:9052; Vector Backbone:pBDP; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18621688
Comments: pBPGUw is a modular Gateway compatible GAL4 vector amenable to high throughput in vitro cloning using Invitrogen LR clonase and specific in vivo genomic targeting using PhiC31 integrase. pBPGUw contains a Drosophila synthetic core promoter (DSCP) that contains TATA, Inr, MTE, and DPE motifs. In addition the GAL4 CDS and yeast transcriptional terminator can easily be substituted for another driver by a directional 5' KpnI to 3' HindIII digest.
Proper citation: RRID:Addgene_17575 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' homology for integration site X-2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176162 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' homology for integration site XII-5
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176176 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3' homology for integration site XI-3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176171 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3' homology for integration site XI-5
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176173 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3' homology for integration site X-4
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176165 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' homology for integration site XI-2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176168 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' homology for integration site X-4
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176164 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3' homology for integration site X-2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176163 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TPK2+ccdB dropout cassette
Vector Backbone Description: Vector Backbone:MoClo assembly; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015) from the part plasmids pYTK002, pMC234r-ccdB/TPK2, pYTK067, pYTK074, pYTK081, pYTK083.
Proper citation: RRID:Addgene_176183 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pST39-Vps25(1-203) - His/Vps22(1-234) - Vps36 (1-567)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pST39; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15329733
Comments: DNAs for Vps25, Vps22, and Vps36 were cloned in order into pST39. Vps22 was tagged with an N-terminal hexahistidine tag and a TEV protease cleavage site; the other subunits were untagged.
Proper citation: RRID:Addgene_17633 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Bro1 (1-387)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:parallel GST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15935782
Comments: The vector backbone, parallel GST2, was made by replacing the polylinker of pGEX4T1 with the polylinker isolated from the baculovirus expression plasmid pFastBac (see PMID: 10024467).
Proper citation: RRID:Addgene_17635 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ub-MPGLW-Fbp1
Vector Backbone Description: Backbone Marker:Alexander Varshavsky's lab; Backbone Size:8017; Vector Backbone:pJO629; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32513738
Proper citation: RRID:Addgene_176622 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: bar1Sc
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The gene was codon-optimized for expression in S. cervisiae and cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176738 Copy
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