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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
Sc NybbR eRF3∆165N (H348E)
 
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RRID:Addgene_175420 eRF3 Saccharomyces cerevisiae Kanamycin PMID:34413231 Backbone Marker:UC Berkeley MacroLab; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin ∆165N, H348E 2026-08-15 01:10:50 0
Sc NybbR eRF1 (WT)
 
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RRID:Addgene_175414 eRF1 Saccharomyces cerevisiae Kanamycin PMID:34413231 Backbone Marker:UC Berkeley MacroLab; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:10:50 0
Sc NybbR eRF1 (G180A)
 
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RRID:Addgene_175415 eRF1 Saccharomyces cerevisiae Kanamycin PMID:34413231 Backbone Marker:UC Berkeley MacroLab; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin G180A 2026-08-15 01:10:50 0
Sc NybbR eRF3∆165N (WT)
 
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RRID:Addgene_175419 eRF3 Saccharomyces cerevisiae Kanamycin PMID:34413231 Backbone Marker:UC Berkeley MacroLab; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin ∆165N 2026-08-15 01:10:50 0
Sc eRF1 (WT)
 
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RRID:Addgene_175413 eRF1 Saccharomyces cerevisiae Kanamycin PMID:34413231 Backbone Marker:UC Berkeley MacroLab; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:10:49 0
pBPGw
 
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1+ mentions
RRID:Addgene_17574 GAL4 Saccharomyces cerevisiae Chloramphenicol and Ampicillin PMID:18621688 pBPGw is a modular Gateway compatible promoter-less GAL4 vector amenable to high throughput in vitro cloning using Invitrogen LR clonase and specific in vivo genomic targeting using PhiC31 integrase. In addition the GAL4 CDS and yeast transcriptional terminator can easily be substituted for another driver by a directional 5' KpnI to 3' HindIII digest. Backbone Size:8902; Vector Backbone:pBDP; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:10:52 7
pBPGUw
 
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10+ mentions
RRID:Addgene_17575 GAL4 Saccharomyces cerevisiae Chloramphenicol and Ampicillin PMID:18621688 pBPGUw is a modular Gateway compatible GAL4 vector amenable to high throughput in vitro cloning using Invitrogen LR clonase and specific in vivo genomic targeting using PhiC31 integrase. pBPGUw contains a Drosophila synthetic core promoter (DSCP) that contains TATA, Inr, MTE, and DPE motifs. In addition the GAL4 CDS and yeast transcriptional terminator can easily be substituted for another driver by a directional 5' KpnI to 3' HindIII digest. Backbone Size:9052; Vector Backbone:pBDP; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:10:52 41
pMC8b-X2up
 
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RRID:Addgene_176162 5' homology for integration site X-2 Saccharomyces cerevisiae Chloramphenicol PMID:34860007 Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:10:56 0
pMC8b-XII5up
 
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RRID:Addgene_176176 5' homology for integration site XII-5 Saccharomyces cerevisiae Chloramphenicol PMID:34860007 Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:10:56 0
pMC7-XI3dw
 
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RRID:Addgene_176171 3' homology for integration site XI-3 Saccharomyces cerevisiae Chloramphenicol PMID:34860007 Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:10:56 0
pMC7-XI5dw
 
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RRID:Addgene_176173 3' homology for integration site XI-5 Saccharomyces cerevisiae Chloramphenicol PMID:34860007 Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:10:56 0
pMC7-X4dw
 
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RRID:Addgene_176165 3' homology for integration site X-4 Saccharomyces cerevisiae Chloramphenicol PMID:34860007 Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:10:56 0
pMC8b-XI2up
 
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RRID:Addgene_176168 5' homology for integration site XI-2 Saccharomyces cerevisiae Chloramphenicol PMID:34860007 Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:10:56 0
pMC8b-X4up
 
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RRID:Addgene_176164 5' homology for integration site X-4 Saccharomyces cerevisiae Chloramphenicol PMID:34860007 Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:10:56 0
pMC7-X2dw
 
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RRID:Addgene_176163 3' homology for integration site X-2 Saccharomyces cerevisiae Chloramphenicol PMID:34860007 Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:10:56 0
pMG343
 
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RRID:Addgene_176183 TPK2+ccdB dropout cassette Saccharomyces cerevisiae Ampicillin PMID:34860007 Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015) from the part plasmids pYTK002, pMC234r-ccdB/TPK2, pYTK067, pYTK074, pYTK081, pYTK083. Vector Backbone:MoClo assembly; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:56 0
ESCRT-II
 
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1+ mentions
RRID:Addgene_17633 pST39-Vps25(1-203) - His/Vps22(1-234) - Vps36 (1-567) Saccharomyces cerevisiae Ampicillin PMID:15329733 DNAs for Vps25, Vps22, and Vps36 were cloned in order into pST39. Vps22 was tagged with an N-terminal hexahistidine tag and a TEV protease cleavage site; the other subunits were untagged. Backbone Size:0; Vector Backbone:pST39; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:57 2
pGST2-Bro1 (1-387)
 
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RRID:Addgene_17635 Bro1 (1-387) Saccharomyces cerevisiae Ampicillin PMID:15935782 The vector backbone, parallel GST2, was made by replacing the polylinker of pGEX4T1 with the polylinker isolated from the baculovirus expression plasmid pFastBac (see PMID: 10024467). Backbone Size:5000; Vector Backbone:parallel GST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:58 0
pCSJ982
 
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RRID:Addgene_176622 Ub-MPGLW-Fbp1 Saccharomyces cerevisiae Ampicillin PMID:32513738 Backbone Marker:Alexander Varshavsky's lab; Backbone Size:8017; Vector Backbone:pJO629; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Changed first 4 N-terminal amino acid residues of Fbp1 to PGLW 2026-08-15 01:11:00 0
pYCTK034
 
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RRID:Addgene_176738 bar1Sc Saccharomyces cerevisiae Chloramphenicol The gene was codon-optimized for expression in S. cervisiae and cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint. Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:11:02 0

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