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Species: Homo sapiens
Genetic Insert: Neurogenin 2
Vector Backbone Description: Backbone Marker:custom; Backbone Size:6000; Vector Backbone:pUCM; Vector Types:CRISPR, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29924488
Comments: Please note that this plasmid may runs as a dimer (>21kb). Concatenation/Dimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_105840 Copy
Species: Homo sapiens
Genetic Insert: Neurogenin 2
Vector Backbone Description: Backbone Marker:custom; Backbone Size:6000; Vector Backbone:pUCM; Vector Types:CRISPR, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29924488
Proper citation: RRID:Addgene_105842 Copy
Species: Homo sapiens
Genetic Insert: EB3
Vector Backbone Description: Vector Backbone:pCS2+ hsp70; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25600237
Proper citation: RRID:Addgene_105948 Copy
Species: Homo sapiens
Genetic Insert: PCNA
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25600237
Proper citation: RRID:Addgene_105942 Copy
Species: Staphylococcus aureus
Genetic Insert: Cas9 nuclease
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29334369
Comments: Addgene NGS results show a C-terminal HA tag rather than 3x HA tag. Also, the mCD4 selection marker matches NCBI reference NP_038516.1 and contains residues 1-427. Plasmids should function as described in the associated publication.
Proper citation: RRID:Addgene_105998 Copy
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, Lentiviral, CRISPR, S.pyogenes sgRNA expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29334369
Comments: Dual sgRNA vector to express one sgRNA for S.pyogenes Cas9 and one sgRNA for S.aureus Cas9. Cloning is done in two steps: 1.) AarI digest to clone the S.pyogenes sgRNA and after preparation of the successfully cloned plasmid 2.) BfuAI digest followed by cloning of the S.aureus sgRNA.
Proper citation: RRID:Addgene_105997 Copy
Species: Homo sapiens
Genetic Insert: dynactin1-811
Vector Backbone Description: Vector Backbone:GWpTolDest(R4-R2)pA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26879757
Comments: The depositor notes that the L287M found in dynactin is not of functional concern.
Proper citation: RRID:Addgene_105970 Copy
Species: Danio rerio
Genetic Insert: Paxillin
Vector Backbone Description: Vector Backbone:GWpCS2 Dest(R1-R3); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28372636
Comments: The depositor notes that the V94A and P149S mutations found in Paxillin are not of functional concern.
Proper citation: RRID:Addgene_105974 Copy
Species: Homo sapiens
Genetic Insert: hSyn-Igkappa-myc-FAPdL5-POST-T2A-dTomato-WPRE-bGH
Vector Backbone Description: Backbone Marker:PENN vector core; Backbone Size:4643; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31548370
Proper citation: RRID:Addgene_105981 Copy
Species: Homo sapiens
Genetic Insert: FLEX-FAPdL5-POST-T2A-dTomato.FLEX
Vector Backbone Description: Backbone Size:4894; Vector Backbone:pAAV-FLEX; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31548370
Proper citation: RRID:Addgene_105982 Copy
Species: Homo sapiens
Genetic Insert: Stathmin1
Vector Backbone Description: Vector Backbone:GWpTol2Dest(R4-R3)pA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26879757
Proper citation: RRID:Addgene_105969 Copy
Species: Homo sapiens
Genetic Insert: GPCR activation based ACh sensor (GACh) 2.0
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7242; Vector Backbone:pDisplay vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29985477
Proper citation: RRID:Addgene_106073 Copy
Species: Homo sapiens
Genetic Insert: NP_001410.2
Vector Backbone Description: Vector Backbone:pFRT_TO_eGFP_dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29429924
Proper citation: RRID:Addgene_106105 Copy
Species: Homo sapiens
Genetic Insert: PRKRA
Vector Backbone Description: Vector Backbone:pLenti_C_Myc_DDK_IRES_Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29429924
Proper citation: RRID:Addgene_106114 Copy
Species: Homo sapiens
Genetic Insert: PRKRA
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29429924
Proper citation: RRID:Addgene_106110 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29377615
Comments: Selectable marker: nourseothricin
Proper citation: RRID:Addgene_106142 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29377615
Comments: Selectable marker: nourseothricin
Proper citation: RRID:Addgene_106143 Copy
Species: Homo sapiens
Genetic Insert: gRNA TIA1
Vector Backbone Description: Vector Backbone:px458; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29429924
Proper citation: RRID:Addgene_106097 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29377615
Proper citation: RRID:Addgene_106130 Copy
Species: Homo sapiens
Genetic Insert: TIA1
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29429924
Proper citation: RRID:Addgene_106095 Copy
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