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Species: Homo sapiens
Genetic Insert: hTRF2
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pLPC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11923537
Comments: {Wang et al., 2004, Cell, 119, 355-68}
Proper citation: RRID:Addgene_18006 Copy
Species: Homo sapiens
Genetic Insert: hTRF2
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pLPC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11923537
Comments: {Wang et al., 2004, Cell, 119, 355-68}
Proper citation: RRID:Addgene_18007 Copy
Species: Homo sapiens
Genetic Insert: hTRF2
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pLPC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11923537
Comments: {Wang et al., 2004, Cell, 119, 355-68}
Proper citation: RRID:Addgene_18008 Copy
Species: Homo sapiens
Genetic Insert: hTRF2
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pWZL Hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11923537
Proper citation: RRID:Addgene_18009 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA H1047R
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pT3-EF1aH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26716908
Proper citation: RRID:Addgene_180031 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA E545K
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26716908
Proper citation: RRID:Addgene_180032 Copy
Species: Homo sapiens
Genetic Insert: hTRF2
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pWZL Hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11923537
Proper citation: RRID:Addgene_18012 Copy
Species: Homo sapiens
Genetic Insert: hTRF2
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pWZL Hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11923537
Proper citation: RRID:Addgene_18013 Copy
Species: Homo sapiens
Genetic Insert: human GPIbalpha 1-290aa with mutation G233V and M239V
Vector Backbone Description: Backbone Size:5341; Vector Backbone:ET8; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30797858
Proper citation: RRID:Addgene_180251 Copy
Species: Homo sapiens
Genetic Insert: p50
Vector Backbone Description: Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27559088
Proper citation: RRID:Addgene_180238 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*24:02
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00050 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180445 Copy
Species: Homo sapiens
Genetic Insert: HLA-B*08:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00146 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180447 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*01:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00001 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180448 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180321 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v3
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180322 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180328 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180329 Copy
Species: Homo sapiens
Genetic Insert: HLA-B*35:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00237 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180450 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*03:01
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEMEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00037 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180451 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*02:01 (CD8 mut)
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Mutation prevents binding of CD8 (Purbhoo et al. (2001) J. Biol. Chem. doi.org/10.1074/jbc.M102498200). See https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00005 for allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180459 Copy
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