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Species: Homo sapiens
Genetic Insert: Human SMN1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16301532
Proper citation: RRID:Addgene_37057 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid #37060; Vector Backbone:pHSS6; Vector Types:; Bacterial Resistance:Kanamycin
Comments: Used to create strains to complement mutations using the NICS system (Mehr and Seifert, 1998; Mehr, 2000). Insert a functional copy of the gene ectopically at an unlinked chromosomal locus between the lctP and aspC genes under control of lac regulatory sequences, and linked to an Erythromycin-resistance cassette.
Proper citation: RRID:Addgene_37058 Copy
Vector Backbone Description: Backbone Size:2320; Vector Backbone:pMB8; Vector Types:Cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:3003748
Proper citation: RRID:Addgene_37060 Copy
Species: Homo sapiens
Genetic Insert: Dsg2
Vector Backbone Description: Backbone Marker:K. Green lab; Vector Backbone:pmCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22184201
Proper citation: RRID:Addgene_36991 Copy
Species: Homo sapiens
Genetic Insert: Dsg2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987342
Comments: The construct encoding human Dsg2 cDNA in pBluescript was a gift from Drs. W. Franke and S. Schafer, German Cancer Research Center, Heidelberg, Germany.
Proper citation: RRID:Addgene_36989 Copy
Species: Mus musculus
Genetic Insert: Mib1(Mus musculus mindbomb homolog 1 (Drosophila) (Mib1))
Vector Backbone Description: Backbone Marker:Agilent (Stratagene); Backbone Size:4319; Vector Backbone:pCMV-Tag4a; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21903422
Proper citation: RRID:Addgene_37116 Copy
Genetic Insert: attL1::egfp (870 bp)::attL2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR 221; Vector Types:Multi-site Gateway donor plasmid; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_42930 Copy
Species: Homo sapiens
Genetic Insert: NKX3.1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET-30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19780584
Comments: This is a full length NKX3.1 cDNA construct. Protein expressed in E.coli BL21(DE3) competent cells
Proper citation: RRID:Addgene_42896 Copy
Species: Strongyloides stercoralis
Genetic Insert: attR2::Ss-era-1 terminator (598 bp)::attL3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4773; Vector Backbone:pDONR P2R-P3; Vector Types:Multi-site Gateway donor plasmid; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_42928 Copy
Species: A. victoria
Genetic Insert: Aquamarine
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23192565
Proper citation: RRID:Addgene_42888 Copy
Genetic Insert: CRISPR-BsaI
Vector Backbone Description: Backbone Size:2254; Vector Backbone:pZE21-MCS1; Vector Types:Bacterial Expression, CRISPR, E.coli; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23360965
Comments: This plasmid is also known as pDB129.
This plasmid contains a duplicated spacer insertion site (4 BsaI sites instead of 2). Please note that these extra sites are eliminated upon treatment with BsaI, and the depositing lab has used this version extensively without issue. Addgene has found that versions of the plasmid with and without the duplicated spacer are present, both are predicted to perform similarly.
The HME63 strain is available from the from the Court Lab:
http://redrecombineering.ncifcrf.gov/strains.html
For more information on Marraffini Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/marraffini/
Proper citation: RRID:Addgene_42875 Copy
Species: Perch (Morone americana)
Genetic Insert: Connexin 35 C-terminal domain
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5930; Vector Backbone:pET42c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15857663
Comments: Stop codon at CamKII phosphorylation site near tip of the C-terminus. Truncates last 7 amino acids.
Proper citation: RRID:Addgene_42913 Copy
Species: Mus musculus
Genetic Insert: PECAM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Compared to GenBank accession number L06039.1, the PECAM-1 sequence in this plasmid contains 2 nucleotide mismatches in the 5' untranslated region (UTR).
Proper citation: RRID:Addgene_42901 Copy
Species: Homo sapiens
Genetic Insert: PECAM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_42902 Copy
Species: Homo sapiens
Genetic Insert: Cry2PHR-mCherry-RhoA
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3991; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23377377
Proper citation: RRID:Addgene_42958 Copy
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22245580
Comments: NOTE: there is a second, non-annotated EcoRI site present in this plasmid. The function of the plasmid will not be impaired; however,
you cannot use the EcoRI site in the MCS for cloning.
Proper citation: RRID:Addgene_43796 Copy
Species: Homo sapiens
Genetic Insert: JIP4
Vector Backbone Description: Backbone Size:6410; Vector Backbone:CMV-mNeonGreen-pDEST; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33177079
Proper citation: RRID:Addgene_164621 Copy
Species: Homo sapiens
Genetic Insert: RAB35
Vector Backbone Description: Backbone Size:6095; Vector Backbone:pCMV-Tag3B-2xMYC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33177079
Proper citation: RRID:Addgene_164632 Copy
Species: Homo sapiens
Genetic Insert: JIP4
Vector Backbone Description: Backbone Size:6095; Vector Backbone:pCMV-Tag3B-2xMYC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33177079
Proper citation: RRID:Addgene_164633 Copy
Species: Homo sapiens
Genetic Insert: mitogen-activated protein kinase kinase 1
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33932340
Proper citation: RRID:Addgene_164638 Copy
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