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Species: Homo sapiens
Genetic Insert: APC
Vector Backbone Description: Backbone Size:0; Vector Backbone:pSAR-MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8755583
Proper citation: RRID:Addgene_16487 Copy
Species: Homo sapiens
Genetic Insert: Pig3
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4500; Vector Backbone:pBK-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9305847
Proper citation: RRID:Addgene_16496 Copy
Genetic Insert: Smad binding element
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9660945
Comments: Four copies of the Smad binding site (GTCTAGAC) are cloned into pBV-Luc.
Proper citation: RRID:Addgene_16495 Copy
Species: Synthetic
Genetic Insert: ccdB cassette
Vector Backbone Description: Backbone Marker:Yingfeng An; Vector Backbone:pANY4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Plasmid containing T7 promoter and pR18/pL18 in the complementary DNA strands for IPTG-induced or temperature-induced protein expression.
Proper citation: RRID:Addgene_164897 Copy
Species: Caenorhabditis elegans
Genetic Insert: rpl28-rtTA(Q)
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_164886 Copy
Species: Synthetic
Genetic Insert: Enhanced green fluorescent protein
Vector Backbone Description: Backbone Size:12176; Vector Backbone:pInducer-10b; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25093678
Proper citation: RRID:Addgene_164925 Copy
Vector Backbone Description: Backbone Size:3489; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD128.110, Ligation number L4417.
Proper citation: RRID:Addgene_1649 Copy
Species: Synthetic
Genetic Insert: POLArISact
Vector Backbone Description: Backbone Marker:Zhao et. al. DOI: 10.1126/science.1208592; Vector Backbone:modified pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33674463
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.06.20.116178v2 full for bioRxiv preprint.
Proper citation: RRID:Addgene_164971 Copy
Species: Homo sapiens
Genetic Insert: gRNA against TRAC
Vector Backbone Description: Vector Backbone:pXPR_001 (lentiCRISPRv1, Addgene #49535); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_164993 Copy
Species: Homo sapiens
Genetic Insert: TRAV
Vector Backbone Description: Backbone Size:9531; Vector Backbone:pLX301 (Addgene #25895); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_164999 Copy
Species: Homo sapiens
Genetic Insert: Rheb
Vector Backbone Description: Backbone Marker:Available at Addgene (#19319); Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33157014
Proper citation: RRID:Addgene_165031 Copy
Species: Homo sapiens
Genetic Insert: BACE1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22553349
Proper citation: RRID:Addgene_165032 Copy
Species: Homo sapiens
Genetic Insert: APC
Vector Backbone Description: Backbone Marker:Vogelstein lab (Addgene plasmid# 16440); Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065402
Proper citation: RRID:Addgene_16507 Copy
Genetic Insert: Smad binding element
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9660945
Comments: Two copies of the Smad binding site (taaGTCTAGACggcaGTCTAGACgtac) are cloned into pGL3.
Proper citation: RRID:Addgene_16500 Copy
Species: Homo sapiens
Genetic Insert: OCT4
Vector Backbone Description: Vector Backbone:pMaster; Vector Types:Mammalian Expression, Episomal; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088009
Comments: Please note that Addgene's NGS found that the bGH poly(A) signal between one of the oris and Lin28 is absent as compared to the annotated sequence provided by the depositor.
Please note that this plasmid contains repetitive elements which are prone to recombination. Screen 3-5 small colonies to ensure the full plasmid is selected. For example, the restriction enzyme NheI can be used to check the plasmid size as shown in Addgene's Diagnostic Digest.
Proper citation: RRID:Addgene_165081 Copy
Species: Ruminoccocus flavefaciens XPD3002
Genetic Insert: RfxCas13d
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33288960
Proper citation: RRID:Addgene_165077 Copy
Species: Other
Genetic Insert: Scaffold gRNA
Vector Backbone Description: Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33288960
Comments: Please note that viral vectors are prone to recombination. Addgene recommends testing 2-4 individual colonies to ensure the full plasmid is intact.
Proper citation: RRID:Addgene_165078 Copy
Species: Ruminoccocus flavefaciens XPD3002
Genetic Insert: RfxCas13d
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33288960
Comments: Please note that the fluorophore is sfGFP. Previous versions of SnapGene may label it as mCFP.
Proper citation: RRID:Addgene_165076 Copy
Genetic Insert: Smad binding element
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9702198
Comments: SBE-luc was made by annealing the oligonucleotides 5′-CGGAGTATGTCTAGACTGACAATG-3′ and 5′-CTAGCATTGTCAGTCTAGACATACTCCGAGCT-3′ and cloning them into the Sac1 and Nhe1 sites.
Proper citation: RRID:Addgene_16527 Copy
Species: Homo sapiens
Genetic Insert: FAST-1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9702198
Comments: To construct an hFAST-1 expression vector, normal human colon cDNA was used as the template to PCR-amplify the hFAST-1 ORF with primers NT2-exp5′ (5′-TATGCGGCCGCCACCATGGGGCCCTGCAGCG-3′) and NT2-exp3′ (5′-TATGCGGCCGCGAGCTGCTGTGTCGCAGAC-3′). The PCR product was cloned into the NotI site of pCI-neo.
Proper citation: RRID:Addgene_16521 Copy
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