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Species: Synthetic
Genetic Insert: sgRNArodA
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149635 Copy
Species: Synthetic
Genetic Insert: Psyn-mCherryBb
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2_2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149636 Copy
Species: Synthetic
Genetic Insert: dCas9, lacI
Vector Backbone Description: Vector Backbone:pCR2.1, heavily modified; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149637 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9K(RBSmut); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149577 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2_2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149613 Copy
Species: Mus musculus
Genetic Insert: Mer
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:5300; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15673687
Comments: The full-length mouse Mer (994 amino acids) was cloned from a 16-day-old mouse embryo cDNA library. Addgene's quality control found the closest match to the insert sequence is mouse Mer (AA17-994) Sequence ID: NP_032613.1, with mutations I516V and Q860R. The plasmid should function as described in the associated article listed on the plasmid page above.
Proper citation: RRID:Addgene_14998 Copy
Species: Homo sapiens
Genetic Insert: human frataxin cDNA
Vector Backbone Description: Backbone Marker:BMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_14992 Copy
Species: Homo sapiens
Genetic Insert: PLAP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pDOL; Vector Types:Mammalian Expression, Retroviral, Avian expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:1731342
Comments: Retroviral vector expressing PALP from the long-terminal-repeat (LTR) promoter. The neomycin gene is expressed from SV40 promoter.
Plasmid CMV-PLAP was digested with KpnI and a 1930bp fragment encoding PLAP was isolated. The ends of this fragment were blunted with T4 DNA polymerase and ligated to a SalI linker. The linker fragment was digested with SalI and inserted into the SalI site of the Moloney murine leukemia virus retroviral vector pDOL.
Proper citation: RRID:Addgene_15164 Copy
Species: Escherichia coli
Genetic Insert: beta-galactosidase
Vector Backbone Description: Backbone Size:0; Vector Backbone:modified pDOL; Vector Types:Mammalian Expression, Retroviral, Avian expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:3099292
Comments: Retroviral vector expressing beta-galactosidase from the long-terminal-repeat (LTR) promoter. The neomycin gene is expressed from SV40 promoter.
Proper citation: RRID:Addgene_15148 Copy
Species: Homo sapiens
Genetic Insert: G protein coupled receptor kinase-interacting protein 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:PEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11896197
Proper citation: RRID:Addgene_15226 Copy
Species: E. coli
Genetic Insert: beta-glucoronidase (E. coli)
Vector Backbone Description: Backbone Marker:Engineering Nitrogen Symbiosis for Africa (ENSA); Backbone Size:2503; Vector Backbone:EC10161; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33072173
Proper citation: RRID:Addgene_154059 Copy
Vector Backbone Description: Backbone Marker:NA; Backbone Size:3103; Vector Backbone:NA; Vector Types:Bacterial Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33072173
Proper citation: RRID:Addgene_154060 Copy
Species: Synthetic
Genetic Insert: engineered bacterial rRNAs operon under control of pL promotor
Vector Backbone Description: Backbone Size:3414; Vector Backbone:Synthetic; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32313034
Proper citation: RRID:Addgene_154125 Copy
Species: Synthetic
Genetic Insert: p40S:QfDBD-VP16AD-GFP-NLS
Vector Backbone Description: Backbone Marker:MoClo Tool Kit of Sylvestre Marillonnet; Backbone Size:8102; Vector Backbone:pAGM4673; Vector Types:Bacterial Expression, Synthetic Biology, Fungal Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31739777
Comments: Synthetic Transcription Factor (QfDBD-VP16AD-GFP-NLS) and DsRed-SKL transcribed with various full and core promoters (CP) with and without Quinic acid Upstream Activation Sequences (QUAS). Fungal selection provided with amdS marker. Flanking regions were designed for integration at the locus of the Penicillin gene cluster.
Proper citation: RRID:Addgene_154228 Copy
Species: Synthetic
Genetic Insert: p40S:QfDBD-VP16AD-GFP-NLS
Vector Backbone Description: Backbone Marker:MoClo Tool Kit of Sylvestre Marillonnet; Backbone Size:8098; Vector Backbone:pAGM4673; Vector Types:Bacterial Expression, Synthetic Biology, Fungal Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31739777
Comments: Synthetic Transcription Factor (QfDBD-VP16AD-GFP-NLS) and DsRed-SKL transcribed with various full and core promoters (CP) with and without Quinic acid Upstream Activation Sequences (QUAS). Fungal selection provided with amdS marker. Flanking regions were designed for integration at the locus of the Penicillin gene cluster.
Proper citation: RRID:Addgene_154224 Copy
Species: Synthetic
Genetic Insert: p40S:QfDBD-VP16AD-GFP-NLS
Vector Backbone Description: Backbone Marker:MoClo Tool Kit of Sylvestre Marillonnet; Backbone Size:8097; Vector Backbone:pAGM4673; Vector Types:Bacterial Expression, Synthetic Biology, Fungal Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31739777
Comments: Synthetic Transcription Factor (QfDBD-VP16AD-GFP-NLS) and DsRed-SKL transcribed with various full and core promoters (CP) with and without Quinic acid Upstream Activation Sequences (QUAS). Fungal selection provided with amdS marker. Flanking regions were designed for integration at the locus of the Penicillin gene cluster.
Proper citation: RRID:Addgene_154223 Copy
Species: Synthetic
Genetic Insert: p40S:QfDBD-VP16AD-GFP-NLS
Vector Backbone Description: Backbone Marker:MoClo Tool Kit of Sylvestre Marillonnet; Backbone Size:8109; Vector Backbone:pAGM4673; Vector Types:Bacterial Expression, Synthetic Biology, Fungal Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31739777
Comments: Synthetic Transcription Factor (QfDBD-VP16AD-GFP-NLS) and DsRed-SKL transcribed with various full and core promoters (CP) with and without Quinic acid Upstream Activation Sequences (QUAS). Fungal selection provided with amdS marker. Flanking regions were designed for integration at the locus of the Penicillin gene cluster.
Proper citation: RRID:Addgene_154235 Copy
Species: Synthetic
Genetic Insert: p40S:QfDBD-VP16AD-GFP-NLS
Vector Backbone Description: Backbone Marker:MoClo Tool Kit of Sylvestre Marillonnet; Backbone Size:8110; Vector Backbone:pAGM4673; Vector Types:Bacterial Expression, Synthetic Biology, Fungal Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31739777
Comments: Synthetic Transcription Factor (QfDBD-VP16AD-GFP-NLS) and DsRed-SKL transcribed with various full and core promoters (CP) with and without Quinic acid Upstream Activation Sequences (QUAS). Fungal selection provided with amdS marker. Flanking regions were designed for integration at the locus of the Penicillin gene cluster.
Proper citation: RRID:Addgene_154236 Copy
Species: Synthetic
Genetic Insert: p40S:QfDBD-VP16AD-GFP-NLS
Vector Backbone Description: Backbone Marker:MoClo Tool Kit of Sylvestre Marillonnet; Backbone Size:8106; Vector Backbone:pAGM4673; Vector Types:Bacterial Expression, Synthetic Biology, Fungal Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31739777
Comments: Synthetic Transcription Factor (QfDBD-VP16AD-GFP-NLS) and DsRed-SKL transcribed with various full and core promoters (CP) with and without Quinic acid Upstream Activation Sequences (QUAS). Fungal selection provided with amdS marker. Flanking regions were designed for integration at the locus of the Penicillin gene cluster.
Proper citation: RRID:Addgene_154232 Copy
Species: Synthetic
Genetic Insert: p40S:QfDBD-VP16AD-GFP-NLS
Vector Backbone Description: Backbone Marker:MoClo Tool Kit of Sylvestre Marillonnet; Backbone Size:8108; Vector Backbone:pAGM4673; Vector Types:Bacterial Expression, Synthetic Biology, Fungal Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31739777
Comments: Synthetic Transcription Factor (QfDBD-VP16AD-GFP-NLS) and DsRed-SKL transcribed with various full and core promoters (CP) with and without Quinic acid Upstream Activation Sequences (QUAS). Fungal selection provided with amdS marker. Flanking regions were designed for integration at the locus of the Penicillin gene cluster.
Proper citation: RRID:Addgene_154234 Copy
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