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Species: Saccharomyces cerevisiae
Genetic Insert: mfalpha1Sc
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The gene was codon-optimized for expression in S. cervisiae and cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176720 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P-FUS1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The part was cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176706 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P-DIG2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The part was cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176705 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P-FUS3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The part was cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176707 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ABP1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177099 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gal4 activation domain plus GGSGGGGSGG linker
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36088516
Comments: the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard, derived from GB0900
Proper citation: RRID:Addgene_177034 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RBD2
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177109 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AUR1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177101 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ENT5
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177102 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: STV1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177111 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC2
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739034
Proper citation: RRID:Addgene_177110 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4 DBD
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4628; Vector Backbone:pIZT; Vector Types:Insect Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Tetracycline
Defining Citation: PMID:19481053
Proper citation: RRID:Addgene_21602 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS2
Vector Backbone Description: Backbone Size:5549; Vector Backbone:pHis2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19234443
Proper citation: RRID:Addgene_21494 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Vps20 Delta_C
Vector Backbone Description: Backbone Size:5549; Vector Backbone:pHis2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19234443
Proper citation: RRID:Addgene_21491 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Kss1-as2-trunc
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19079053
Proper citation: RRID:Addgene_21790 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CFP-Ste12
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pACL7-YFP-A206K; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19079053
Comments: Construction was complicated; please refer to parent article's supplemental materials for details of construction. KpnI site is not unique in this plasmid.
Proper citation: RRID:Addgene_21787 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAD5
Vector Backbone Description: Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18757916
Comments: Mutation inactivates the ATPase and DNA helicase activities of RAD5
Proper citation: RRID:Addgene_22290 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADE1
Vector Backbone Description: Backbone Marker:R. D. Gietz; Backbone Size:5741; Vector Backbone:YCplac181; Vector Types:yeast shuttle; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_22495 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADE1
Vector Backbone Description: Backbone Marker:R. D. Gietz; Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:yeast shuttle; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_22494 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: UBR1
Vector Backbone Description: Backbone Size:5741; Vector Backbone:Yeplac 181; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18566452
Comments: Plasmid for overexpressing Ubr1ha in yeast. The ha tag is at the C-terminus of the protein and the protein is active in degrading N-end rule substrates.
Proper citation: RRID:Addgene_20198 Copy
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